JCB logo
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 996K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bloch, D. P.
Right arrow Articles by Godman, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bloch, D. P.
Right arrow Articles by Godman, G. C.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
J. Biophys. and Biochem. Cytol., Vol 1, 17-28, Copyright © 1955 by Rockefeller University Press

ARTICLE

A MICROPHOTOMETRIC STUDY OF THE SYNTHESES OF DESOXYRIBONUCLEIC ACID AND NUCLEAR HISTONE

David P. Bloch Ph.D.1 and Gabriel C. Godman M.D.1

1 (From the Department of Surgical Pathology, College of Physicians and Surgeons, Columbia University, New York)

1. The fast green stain of Alfert and Geschwind for nuclear basic protein is shown to obey the Beer-Lambert laws when used on purified histone. Interference from acid substances other than nucleic acids as a possible source of error is indicated.

2. Use of this technique after a modified Feulgen stain enables determination of relative amounts of desoxyribonucleic acid and histone in the same individual cells.

3. DNA and histone are shown to have the same distribution in formalin-fixed nuclei.

4. The syntheses of DNA and histone proceed simultaneously resulting in the doubling of both these substances prior to cell division.

5. The standard error for histone values is greater than that for DNA; however, the source of this variability is not known.

 Submitted on October 11, 1954


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents