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J. Biophys. and Biochem. Cytol., Vol 1, 173-182, Copyright © 1955 by Rockefeller University Press

ARTICLE

A CHEMICAL AND HISTOCHEMICAL STUDY OF THE LIPIDES OF THE PYLORIC CECUM OF THE STARFISH, ASTERIAS FORBESI

Manfred L. Karnovsky Ph.D.1, Susan Skilling Jeffrey 1, Michaela Smits Thompson 1, and Helen Wendler Deane Ph.D.1

1 (From the Departments of Biological Chemistry and of Anatomy, and the Biophysical Laboratory, Harvard Medical School, Boston)

The lipides of the diverticula of Asterias forbesi have been studied by histochemical and biochemical means.

Correlations between results obtained by histochemical examination of sections, and chemical analysis of isolated lipide have been made, particularly with respect to phosphatides, steroids, and aldehyde lipides.

The results of the histochemical study were in good agreement with the chemical data as to the nature of the phosphatide fraction, the presence of acetone-soluble aldehyde lipides, and the composition of the free droplet fat.

Homogenized diverticula were differentially centrifuged in order to establish the distribution of types of lipides in the various cellular components.

In addition, data have been presented which demonstrate a direct correlation between the titer of alpha-glycerol ethers and that of acetone-soluble lipide acetals in the unsaponifiable fraction.

 Submitted on October 25, 1954


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