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Fluorescence In Vivo Endomicroscopy
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J. Biophys. and Biochem. Cytol., Vol 1, 237-244, Copyright © 1955 by Rockefeller University Press

ARTICLE

EFFECTS OF CORTISONE ON RAT LIVER MITOCHONDRIA

Charles Upton Lowe M.D.1, Deloris MacKinney 1, and Daljit Sarkaria Ph.D.1

1 (From the Statler Research Laboratories of the Children's Hospital and the Department of Pediatrics, University of Buffalo School of Medicine, Buffalo)

Experiments were designed to determine the effects of parenteral administration of cortisone on mitochondrial numbers of rat liver cells. Observations were made in three ways: (1) after osmic acid fixation and appropriate stain, (2) by phase microscopy on intact cells, (3) by actual enumeration of mitochondria and nuclei to provide data on mitochondria per cell. The results of the experiments indicate that the mitochondria per cell are significantly reduced under the conditions used. Each experimental approach provided similar data, and each tended to support the results obtained from the others.

Submitted on December 23, 1954


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