THE FINE STRUCTURE OF THE GALL BLADDER EPITHELIUM OF THE MOUSE

doi:10.1083/jcb.1.5.445

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ARTICLE

THE FINE STRUCTURE OF THE GALL BLADDER EPITHELIUM OF THE MOUSE

Eichi Yamada M.D.¹

¹ (From the Department of Anatomy, University of Washington, Seattle)

Sections of mouse gall bladder epithelium fixed by perfusionwith buffered osmium tetroxide have been studied in the electronmicroscope as an example of simple columnar epithelium.

Thefree surface presents many microvilli, each presenting a densetip, the capitulum, and displaying a radiating corona of delicatefilaments, the antennulae microvillares. Very small pit-likedepressions, representing caveolae intracellulares, are encounteredalong the cell membrane of the microvilli.

The free cell surfacebetween microvilli shows larger cave-like depressions, likewiserepresenting caveolae intracellulares, containing a dense material.

Thelateral cell borders are extensively folded into pleats, whichdo not interdigitate extensively with corresponding folds ofthe adjacent cell membrane.

The terminal bars are shown to consistof thickened densities of the cell membrane itself in the regionof insertion of the lateral cell wall with the free cell surface.This thickening is associated with an accumulation of densecytoplasmic material in the immediate vicinity. The terminalbar is thus largely a cytoplasmic and cell membrane structure,rather than being primarily intercellular in nature.

The basalcell membrane is relatively straight except for a conical eminencenear the center of the cell, projecting slightly into the underlyingtunica propria. The basal cell membrane itself is overlain bya delicate limiting membrane, which does not follow the lateralcontours of the cell.

Unmyelinated intercellular nerve terminalswith synaptic vesicles have been encountered between the lateralwalls of epithelial cells.

A division of the gall bladder epithelialcell into five zones according to Ferner has been found to beconvenient for this study.

The following cytoplasmic componentshave been noted, and their distribution and appearance described:dense absorption granules, mitochondria, Golgi or agranularmembranes, endoplasmic reticulum or ergastoplasm, ring figures,and irregular dense bodies, perhaps lipoid in nature. The nucleusof these cells is also described.

Submitted on June 15, 1955

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