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J. Biophys. and Biochem. Cytol., Vol 10, 467-474, Copyright © 1961 by Rockefeller University Press

ARTICLE

THE DIRECT ISOLATION OF THE MITOTIC APPARATUS

Daniel Mazia Ph.D.1, J. M. Mitchison Ph.D.1, Heitor Medina M.D.1, and Patricia Harris 1

1 From the Department of Zoology, University of California, Berkeley, California

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

Submitted on March 23, 1961


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