Synergistic effects of cyclic AMP and nerve growth factor on neurite outgrowth and microtubule stability of PC12 cells

doi:10.1083/jcb.100.3.916

This Article

	Full Text (PDF, 5709K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Heidemann, S. R.
	Articles by Bothwell, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Heidemann, S. R.
	Articles by Bothwell, M.


Social Bookmarking

	What's this?

ARTICLES

Synergistic effects of cyclic AMP and nerve growth factor on neurite outgrowth and microtubule stability of PC12 cells

SR Heidemann, HC Joshi, A Schechter, JR Fletcher and M Bothwell

The outgrowth of neurites from rat PC12 cells stimulated by combined treatment of nerve growth factor (NGF) with cAMP is significantly more rapid and extensive than the outgrowth induced by either factor alone. We have compared the responses of PC12 cells under three different growth conditions, NGF alone, cAMP alone, and combined treatment, with respect to surface morphology, rapidity of neurite outgrowth, and stability of neurite microtubules, to understand the synergistic action of NGF and cAMP on PC12. Surface events at early times in these growth conditions varied, suggesting divergent pathways of action of NGF and cAMP. This suggestion is strongly supported by the finding that cells exposed to saturating levels of dibutyryl cAMP without substantial neurite outgrowth initiated neurites within 5 min of NGF. This response has been adopted as a convenient assay for NGF. Neurites that regenerated in the three growth conditions showed marked differences in stability to treatments that depolymerize microtubules. The results indicate that microtubules in cells treated with both NGF and cAMP are significantly more stable than in either growth factor alone. We suggest that a shift of the assembly equilibrium favoring tubulin assembly is a necessary prerequisite for the initiation of neurites by PC12.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Stessin, A. M., Zippin, J. H., Kamenetsky, M., Hess, K. C., Buck, J., Levin, L. R. (2006). Soluble Adenylyl Cyclase Mediates Nerve Growth Factor-induced Activation of Rap1. J. Biol. Chem. 281: 17253-17258 [Abstract] [Full Text]
Wasilewska-Sampaio, A. P., Silveira, M. S., Holub, O., Goecking, R., Gomes, F. C. A., Neto, V. M., Linden, R., Ferreira, S. T., De Felice, F. G. (2005). Neuritogenesis and neuronal differentiation promoted by 2,4-dinitrophenol, a novel anti-amyloidogenic compound. FASEB J. 19: 1627-1636 [Abstract] [Full Text]
Parran, D. K., Barker, A., Ehrich, M. (2005). Effects of Thimerosal on NGF Signal Transduction and Cell Death in Neuroblastoma Cells. Toxicol Sci 86: 132-140 [Abstract] [Full Text]
Piiper, A., Dikic, I., Lutz, M. P., Leser, J., Kronenberger, B., Elez, R., Cramer, H., Muller-Esterl, W., Zeuzem, S. (2002). Cyclic AMP Induces Transactivation of the Receptors for Epidermal Growth Factor and Nerve Growth Factor, Thereby Modulating Activation of MAP Kinase, Akt, and Neurite Outgrowth in PC12 Cells. J. Biol. Chem. 277: 43623-43630 [Abstract] [Full Text]
Sewer, M. B., Waterman, M. R. (2002). Adrenocorticotropin/Cyclic Adenosine 3',5'-Monophosphate-Mediated Transcription of the Human CYP17 Gene in the Adrenal Cortex Is Dependent on Phosphatase Activity. Endocrinology 143: 1769-1777 [Abstract] [Full Text]
Romero, C., Paredes, A., Dissen, G. A., Ojeda, S. R. (2002). Nerve Growth Factor Induces the Expression of Functional FSH Receptors in Newly Formed Follicles of the Rat Ovary. Endocrinology 143: 1485-1494 [Abstract] [Full Text]
Leemhuis, J., Boutillier, S., Schmidt, G., Meyer, D. K. (2002). The Protein Kinase A Inhibitor H89 Acts on Cell Morphology by Inhibiting Rho Kinase. J. Pharmacol. Exp. Ther. 300: 1000-1007 [Abstract] [Full Text]
Parran, D. K., Mundy, W. R., Barone, S. Jr. (2001). Effects of Methylmercury and Mercuric Chloride on Differentiation and Cell Viability in PC12 Cells. Toxicol Sci 59: 278-290 [Abstract] [Full Text]
MacNicol, M. C., MacNicol, A. M. (1999). Nerve Growth Factor-stimulated B-Raf Catalytic Activity Is Refractory to Inhibition by cAMP-dependent Protein Kinase. J. Biol. Chem. 274: 13193-13197 [Abstract] [Full Text]
Zou, Y., Komuro, I., Yamazaki, T., Kudoh, S., Uozumi, H., Kadowaki, T., Yazaki, Y. (1999). Both Gs and Gi Proteins Are Critically Involved in Isoproterenol-induced Cardiomyocyte Hypertrophy. J. Biol. Chem. 274: 9760-9770 [Abstract] [Full Text]
Yao, H., York, R. D., Misra-Press, A., Carr, D. W., Stork, P. J.�S. (1998). The Cyclic Adenosine Monophosphate-dependent Protein Kinase (PKA) Is Required for the Sustained Activation of Mitogen-activated Kinases and Gene Expression by Nerve Growth Factor. J. Biol. Chem. 273: 8240-8247 [Abstract] [Full Text]
Kita, Y, Kimura, K., Kobayashi, M, Ihara, S, Kaibuchi, K, Kuroda, S, Ui, M, Iba, H, Konishi, H, Kikkawa, U, Nagata, S, Fukui, Y (1998). Microinjection of activated phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells through the Rac-JNK signal transduction pathway. J. Cell Sci. 111: 907-915 [Abstract]
Kobayashi, M., Nagata, S., Kita, Y., Nakatsu, N., Ihara, S., Kaibuchi, K., Kuroda, S., Ui, M., Iba, H., Konishi, H., Kikkawa, U., Saitoh, I., Fukui, Y. (1997). Expression of a Constitutively Active Phosphatidylinositol 3-Kinase Induces Process Formation in Rat PC12 Cells. USE OF Cre/loxP RECOMBINATION SYSTEM. J. Biol. Chem. 272: 16089-16092 [Abstract] [Full Text]
Lamoureux, P, Altun-Gultekin, Z., Lin, C, Wagner, J., Heidemann, S. (1997). Rac is required for growth cone function but not neurite assembly. J. Cell Sci. 110: 635-641 [Abstract]
Creedon, D. J., Johnson, E. M. Jr., Lawrence, J. C. Jr. (1996). Mitogen-activated Protein Kinase-independent Pathways Mediate the Effects of Nerve Growth Factor and cAMP on Neuronal Survival. J. Biol. Chem. 271: 20713-20718 [Abstract] [Full Text]
Yao, H., Labudda, K., Rim, C., Capodieci, P., Loda, M., Stork, P. J. S. (1995). Cyclic Adenosine Monophosphate Can Convert Epidermal Growth Factor into a Differentiating Factor in Neuronal Cells. J. Biol. Chem. 270: 20748-20753 [Abstract] [Full Text]
Hara, M, Yaar, M, Tang, A, Eller, M., Reenstra, W, Gilchrest, B. (1994). Role of integrins in melanocyte attachment and dendricity. J. Cell Sci. 107: 2739-2748 [Abstract]
Esmaeli-Azad, B, McCarty, J., Feinstein, S. (1994). Sense and antisense transfection analysis of tau function: tau influences net microtubule assembly, neurite outgrowth and neuritic stability. J. Cell Sci. 107: 869-879 [Abstract]
Edwards, J., Campbell, G, Carr, M, Edwards, C. (1993). Shapes of cells spreading on fibronectin: measurement of the stellation of BHK21 cells induced by raising cyclic AMP, and of its reversal by serum and lysophosphatidic acid. J. Cell Sci. 104: 399-407 [Abstract]