A motile Chlamydomonas flagellar mutant that lacks outer dynein arms

doi:10.1083/jcb.100.4.1228

This Article

	Full Text (PDF, 2061K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Mitchell, D. R.
	Articles by Rosenbaum, J. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Mitchell, D. R.
	Articles by Rosenbaum, J. L.


Social Bookmarking

	What's this?

ARTICLES

A motile Chlamydomonas flagellar mutant that lacks outer dynein arms

DR Mitchell and JL Rosenbaum

A new Chlamydomonas flagellar mutant, pf-28, which swims more slowly than wild-type cells, was selected. Thin-section electron microscopy revealed the complete absence of outer-row dynein arms in this mutant, whereas inner-row arms and other axonemal structures appeared normal. SDS PAGE analysis also indicated that polypeptides previously identified as outer-arm dynein components are completely absent in pf- 28. The two ATPases retained by this mutant sediment at 17.7S and 12.7S on sucrose gradients that contain 0.6 M KCl. Overall swimming patterns of pf-28 differ little from wild-type except that forward swimming speed is reduced to 35% of the wild-type value, and cells show little or no backward movement during photophobic avoidance. Mutant cells will respond to phototactic stimuli, and their flagella will beat in either the forward or reverse mode. This is the first report of a mutant that lacks dynein arms that can swim.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Ahmed, N. T., Gao, C., Lucker, B. F., Cole, D. G., Mitchell, D. R. (2008). ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. JCB 183: 313-322 [Abstract] [Full Text]
Liu, Z., Takazaki, H., Nakazawa, Y., Sakato, M., Yagi, T., Yasunaga, T., King, S. M., Kamiya, R. (2008). Partially Functional Outer-Arm Dynein in a Novel Chlamydomonas Mutant Expressing a Truncated {gamma} Heavy Chain. Eukaryot Cell 7: 1136-1145 [Abstract] [Full Text]
Sakato, M., Sakakibara, H., King, S. M. (2007). Chlamydomonas Outer Arm Dynein Alters Conformation in Response to Ca2+. Mol. Biol. Cell 18: 3620-3634 [Abstract] [Full Text]
Freshour, J., Yokoyama, R., Mitchell, D. R. (2007). Chlamydomonas Flagellar Outer Row Dynein Assembly Protein Oda7 Interacts with Both Outer Row and I1 Inner Row Dyneins. J. Biol. Chem. 282: 5404-5412 [Abstract] [Full Text]
Pazour, G J, Agrin, N, Walker, B L, Witman, G B (2006). Identification of predicted human outer dynein arm genes: candidates for primary ciliary dyskinesia genes. J. Med. Genet. 43: 62-73 [Abstract] [Full Text]
Ahmed, N. T., Mitchell, D. R. (2005). ODA16p, a Chlamydomonas Flagellar Protein Needed for Dynein Assembly. Mol. Biol. Cell 16: 5004-5012 [Abstract] [Full Text]
Okita, N., Isogai, N., Hirono, M., Kamiya, R., Yoshimura, K. (2005). Phototactic activity in Chlamydomonas 'non-phototactic' mutants deficient in Ca2+-dependent control of flagellar dominance or in inner-arm dynein. J. Cell Sci. 118: 529-537 [Abstract] [Full Text]
DiBella, L. M., Sakato, M., Patel-King, R. S., Pazour, G. J., King, S. M. (2004). The LC7 Light Chains of Chlamydomonas Flagellar Dyneins Interact with Components Required for Both Motor Assembly and Regulation. Mol. Biol. Cell 15: 4633-4646 [Abstract] [Full Text]
Wirschell, M., Pazour, G., Yoda, A., Hirono, M., Kamiya, R., Witman, G. B. (2004). Oda5p, a Novel Axonemal Protein Required for Assembly of the Outer Dynein Arm and an Associated Adenylate Kinase. Mol. Biol. Cell 15: 2729-2741 [Abstract] [Full Text]
Wargo, M. J., McPeek, M. A., Smith, E. F. (2004). Analysis of microtubule sliding patterns in Chlamydomonas flagellar axonemes reveals dynein activity on specific doublet microtubules. J. Cell Sci. 117: 2533-2544 [Abstract] [Full Text]
DiBella, L. M., Smith, E. F., Patel-King, R. S., Wakabayashi, K.-i., King, S. M. (2004). A Novel Tctex2-related Light Chain Is Required for Stability of Inner Dynein Arm I1 and Motor Function in the Chlamydomonas Flagellum. J. Biol. Chem. 279: 21666-21676 [Abstract] [Full Text]
Sakato, M., King, S. M. (2003). Calcium Regulates ATP-sensitive Microtubule Binding by Chlamydomonas Outer Arm Dynein. J. Biol. Chem. 278: 43571-43579 [Abstract] [Full Text]
Casey, D. M., Yagi, T., Kamiya, R., Witman, G. B. (2003). DC3, the Smallest Subunit of the Chlamydomonas Flagellar Outer Dynein Arm-docking Complex, Is a Redox-sensitive Calcium-binding Protein. J. Biol. Chem. 278: 42652-42659 [Abstract] [Full Text]
Casey, D. M., Inaba, K., Pazour, G. J., Takada, S., Wakabayashi, K.-i., Wilkerson, C. G., Kamiya, R., Witman, G. B. (2003). DC3, the 21-kDa Subunit of the Outer Dynein Arm-Docking Complex (ODA-DC), Is a Novel EF-Hand Protein Important for Assembly of Both the Outer Arm and the ODA-DC. Mol. Biol. Cell 14: 3650-3663 [Abstract] [Full Text]
Perrone, C. A., Tritschler, D., Taulman, P., Bower, R., Yoder, B. K., Porter, M. E. (2003). A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells. Mol. Biol. Cell 14: 2041-2056 [Abstract] [Full Text]
Wargo, M. J., Smith, E. F. (2003). Asymmetry of the central apparatus defines the location of active microtubule sliding in Chlamydomonas flagella. Proc. Natl. Acad. Sci. USA 100: 137-142 [Abstract] [Full Text]
Neesen, J., Kirschner, R., Ochs, M., Schmiedl, A., Habermann, B., Mueller, C., Holstein, A. F., Nuesslein, T., Adham, I., Engel, W. (2001). Disruption of an inner arm dynein heavy chain gene results in asthenozoospermia and reduced ciliary beat frequency. Hum Mol Genet 10: 1117-1128 [Abstract] [Full Text]
Iomini, C., Babaev-Khaimov, V., Sassaroli, M., Piperno, G. (2001). Protein Particles in Chlamydomonas Flagella Undergo a Transport Cycle Consisting of Four Phases. JCB 153: 13-24 [Abstract] [Full Text]
Perrone, C. A., Myster, S. H., Bower, R., O'Toole, E. T., Porter, M. E. (2000). Insights into the Structural Organization of the I1 Inner Arm Dynein from a Domain Analysis of the 1beta Dynein Heavy Chain. Mol. Biol. Cell 11: 2297-2313 [Abstract] [Full Text]
Pazour, G. J., Koutoulis, A., Benashski, S. E., Dickert, B. L., Sheng, H., Patel-King, R. S., King, S. M., Witman, G. B. (1999). LC2, the Chlamydomonas Homologue of the t Complex-encoded Protein Tctex2, Is Essential for Outer Dynein Arm Assembly. Mol. Biol. Cell 10: 3507-3520 [Abstract] [Full Text]
Myster, S. H., Knott, J. A., Wysocki, K. M., O'Toole, E., Porter, M. E. (1999). Domains in the 1{alpha} Dynein Heavy Chain Required for Inner Arm Assembly and Flagellar Motility in Chlamydomonas. JCB 146: 801-818 [Abstract] [Full Text]
Yang, P., Sale, W. S. (1998). The Mr 140,000�Intermediate Chain of Chlamydomonas Flagellar Inner Arm Dynein Is a WD-Repeat Protein Implicated in Dynein Arm Anchoring. Mol. Biol. Cell 9: 3335-3349 [Abstract] [Full Text]
Perrone, C. A., Yang, P., O'Toole, E., Sale, W. S., Porter, M. E. (1998). The Chlamydomonas IDA7 Locus Encodes a 140-kDa Dynein Intermediate Chain Required to Assemble the I1 Inner Arm Complex. Mol. Biol. Cell 9: 3351-3365 [Abstract] [Full Text]
Fowkes, M. E., Mitchell, D. R. (1998). The Role of Preassembled Cytoplasmic Complexes in Assembly of Flagellar Dynein Subunits. Mol. Biol. Cell 9: 2337-2347 [Abstract] [Full Text]
Koutoulis, A., Pazour, G. J., Wilkerson, C. G., Inaba, K., Sheng, H., Takada, S., Witman, G. B. (1997). The Chlamydomonas reinhardtii ODA3 Gene Encodes a Protein of the Outer Dynein Arm Docking Complex. JCB 137: 1069-1080 [Abstract] [Full Text]
King, S. J., Dutcher, S. K. (1997). Phosphoregulation of an Inner Dynein Arm Complex in Chlamydomonas reinhardtii Is Altered in Phototactic Mutant Strains. JCB 136: 177-191 [Abstract] [Full Text]
King, S., Patel-King, R. (1995). Identification of a Ca(2+)-binding light chain within Chlamydomonas outer arm dynein. J. Cell Sci. 108: 3757-3764 [Abstract]
Moscatelli, A, Del Casino, C, Lozzi, L, Cai, G, Scali, M, Tiezzi, A, Cresti, M (1995). High molecular weight polypeptides related to dynein heavy chains in Nicotiana tabacum pollen tubes. J. Cell Sci. 108: 1117-1125 [Abstract]
Wilkerson, C., King, S., Witman, G. (1994). Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer-arm dynein. J. Cell Sci. 107: 497-506 [Abstract]
Yokota, E, Mabuchi, I (1994). C/A dynein isolated from sea urchin sperm flagellar axonemes. Enzymatic properties and interaction with microtubules. J. Cell Sci. 107: 353-361 [Abstract]
Mitchell, D., Kang, Y (1993). Reversion analysis of dynein intermediate chain function. J. Cell Sci. 105: 1069-1078 [Abstract]
Kagami, O., Kamiya, R. (1992). Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein. J. Cell Sci. 103: 653-664 [Abstract]