Reversible chromosome condensation induced in Drosophila embryos by anoxia: visualization of interphase nuclear organization

doi:10.1083/jcb.100.5.1623

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Reversible chromosome condensation induced in Drosophila embryos by anoxia: visualization of interphase nuclear organization

VE Foe and BM Alberts

We have studied the morphology of nuclei in Drosophila embryos during the syncytial blastoderm stages. Nuclei in living embryos were viewed with differential interference-contrast optics; in addition, both isolated nuclei and fixed preparations of whole embryos were examined after staining with a DNA-specific fluorescent dye. We find that: (a) The nuclear volumes increase dramatically during interphase and then decrease during prophase of each nuclear cycle, with the magnitude of the nuclear volume increase being greatest for those cycles with the shortest interphase. (b) Oxygen deprivation of embryos produces a rapid developmental arrest that is reversible upon reaeration. During this arrest, interphase chromosomes condense against the nuclear envelope and the nuclear volumes increase dramatically. In these nuclei, individual chromosomes are clearly visible, and each condensed chromosome can be seen to adhere along its entire length to the inner surface of the swollen nuclear envelope, leaving the lumen of the nucleus devoid of DNA. (c) In each interphase nucleus the chromosomes are oriented in the "telophase configuration," with all centromeres and all telomeres at opposite poles of the nucleus; all nuclei at the embryo periphery (with the exception of the pole cell nuclei) are oriented with their centromeric poles pointing to the embryo exterior.
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Thakar, R., Gordon, G., Csink, A. K. (2006). Dynamics and anchoring of heterochromatic loci during development. J. Cell Sci. 119: 4165-4175 [Abstract] [Full Text]
Sollid, J., Kjernsli, A., De Angelis, P. M., Rohr, A. K., Nilsson, G. E. (2005). Cell proliferation and gill morphology in anoxic crucian carp. Am. J. Physiol. Regul. Integr. Comp. Physiol. 289: R1196-R1201 [Abstract] [Full Text]
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Gorr, T. A., Tomita, T., Wappner, P., Bunn, H. F. (2004). Regulation of Drosophila Hypoxia-inducible Factor (HIF) Activity in SL2 Cells: IDENTIFICATION OF A HYPOXIA-INDUCED VARIANT ISOFORM OF THE HIF{alpha} HOMOLOG GENE similar. J. Biol. Chem. 279: 36048-36058 [Abstract] [Full Text]
Nystul, T. G., Goldmark, J. P., Padilla, P. A., Roth, M. B. (2003). Suspended Animation in C. elegans Requires the Spindle Checkpoint. Science 302: 1038-1041 [Abstract] [Full Text]
Douglas, R. M., Haddad, G. G. (2003). Genetic Models in Applied Physiology: Invited Review: Effect of oxygen deprivation on cell cycle activity: a profile of delay and arrest. J. Appl. Physiol. 94: 2068-2083 [Abstract] [Full Text]
Mason, J. M., Konev, A. Y., Golubovsky, M. D., Biessmann, H. (2003). Cis- and trans-acting Influences on Telomeric Position Effect in Drosophila melanogaster Detected With a Subterminal Transgene. Genetics 163: 917-930 [Abstract] [Full Text]
Ostashevsky, J. (2002). A Polymer Model for Large-scale Chromatin Organization in Lower Eukaryotes. Mol. Biol. Cell 13: 2157-2169 [Abstract] [Full Text]
Padilla, P. A., Nystul, T. G., Zager, R. A., Johnson, A. C.M., Roth, M. B. (2002). Dephosphorylation of Cell Cycle-regulated Proteins Correlates with Anoxia-induced Suspended Animation in Caenorhabditis elegans. Mol. Biol. Cell 13: 1473-1483 [Abstract] [Full Text]
Fuchs, J., Lorenz, A., Loidl, J. (2002). Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes. J. Cell Sci. 115: 1213-1220 [Abstract] [Full Text]
Padilla, P. A., Roth, M. B. (2001). Oxygen deprivation causes suspended animation in the zebrafish embryo. Proc. Natl. Acad. Sci. USA 10.1073/pnas.131213198v1 [Abstract] [Full Text]
Douglas, R. M., Xu, T., Haddad, G. G. (2001). Cell cycle progression and cell division are sensitive to hypoxia in Drosophila melanogaster embryos. Am. J. Physiol. Regul. Integr. Comp. Physiol. 280: R1555-R1563 [Abstract] [Full Text]
Van Voorhies, W., Ward, S (2000). Broad oxygen tolerance in the nematode Caenorhabditis elegans. J. Exp. Biol. 203: 2467-2478 [Abstract]
Foe, V., Field, C., Odell, G. (2000). Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos. Development 127: 1767-1787 [Abstract]
Csink, A. K., Henikoff, S. (1998). Large-scale Chromosomal Movements During Interphase Progression in Drosophila. JCB 143: 13-22 [Abstract] [Full Text]
Fung, J. C., Marshall, W. F., Dernburg, A., Agard, D. A., Sedat, J. W. (1998). Homologous Chromosome Pairing in Drosophila melanogaster Proceeds through Multiple Independent Initiations. JCB 141: 5-20 [Abstract] [Full Text]
Lu, B., Ma, J, Eissenberg, J. (1998). Developmental regulation of heterochromatin-mediated gene silencing in Drosophila. Development 125: 2223-2234 [Abstract]
Haddad, G. G., Sun, Y.-a., Wyman, R. J., Xu, T. (1997). Genetic basis of tolerance to O2 deprivation in Drosophila�melanogaster. Proc. Natl. Acad. Sci. USA 94: 10809-10812 [Abstract] [Full Text]
Cenci, G, Rawson, R B, Belloni, G, Castrillon, D H, Tudor, M, Petrucci, R, Goldberg, M L, Wasserman, S A, Gatti, M (1997). UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior.. Genes Dev. 11: 863-875 [Abstract]
Torok, T, Harvie, P D, Buratovich, M, Bryant, P J (1997). The product of proliferation disrupter is concentrated at centromeres and required for mitotic chromosome condensation and cell proliferation in Drosophila.. Genes Dev. 11: 213-225 [Abstract]
Frankel, S, Sigel, E., Craig, C, Elgin, S., Mooseker, M., Artavanis-Tsakonas, S (1997). An actin-related protein in Drosophila colocalizes with heterochromatin protein 1 in pericentric heterochromatin. J. Cell Sci. 110: 1999-2012 [Abstract]
Bedoyan, J. K., Lejnine, S., Makarov, V. L., Langmore, J. P. (1996). Condensation of Rat Telomere-specific Nucleosomal Arrays Containing Unusually Short DNA Repeats and Histone H1. J. Biol. Chem. 271: 18485-18493 [Abstract] [Full Text]
Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G., Earnshaw, W. C. (1995). The Centromere: Hub of Chromosomal Activities. Science 270: 1591-1594 [Abstract]
Gunawardena, S, Heddle, E, Rykowski, M. (1995). 'Chromosomal puffing' in diploid nuclei of Drosophila melanogaster. J. Cell Sci. 108: 1863-1872 [Abstract]
Maldonado-Codina, G, Llamazares, S, Glover, D. (1993). Heat shock results in cell cycle delay and synchronisation of mitotic domains in cellularised Drosophila melanogaster embryos. J. Cell Sci. 105: 711-720 [Abstract]
Davis, I., Francis-Lang, H., Ish-Horowicz, D. (1993). Mechanisms of Intracellular Transcript Localization and Export in Early Drosophila Embryos. Cold Spring Harb Symp Quant Biol 58: 793-798 [Abstract]
DiGregorio, P. J., Ubersax, J. A., O'Farrell, P. H. (2001). Hypoxia and Nitric Oxide Induce a Rapid, Reversible Cell Cycle Arrest of the Drosophila Syncytial Divisions. J. Biol. Chem. 276: 1930-1937 [Abstract] [Full Text]
Padilla, P. A., Roth, M. B. (2001). Oxygen deprivation causes suspended animation in the zebrafish embryo. Proc. Natl. Acad. Sci. USA 98: 7331-7335 [Abstract] [Full Text]