Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes

doi:10.1083/jcb.101.3.942

This Article

	Full Text (PDF, 937K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pan, B. T.
	Articles by Johnstone, R. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pan, B. T.
	Articles by Johnstone, R. M.


Social Bookmarking

	What's this?

ARTICLES

Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes

BT Pan, K Teng, C Wu, M Adam and RM Johnstone

Using ferritin-labeled protein A and colloidal gold-labeled anti-rabbit IgG, the fate of the sheep transferrin receptor has been followed microscopically during reticulocyte maturation in vitro. After a few minutes of incubation at 37 degrees C, the receptor is found on the cell surface or in simple vesicles of 100-200 nm, in which the receptor appears to line the limiting membrane of the vesicles. With time (60 min or longer), large multivesicular elements (MVEs) appear whose diameter may reach 1-1.5 micron. Inside these large MVEs are round bodies of approximately 50-nm diam that bear the receptor at their external surfaces. The limiting membrane of the large MVEs is relatively free from receptor. When the large MVEs fuse with the plasma membrane, their contents, the 50-nm bodies, are released into the medium. The 50-nm bodies appear to arise by budding from the limiting membrane of the intracellular vesicles. Removal of surface receptor with pronase does not prevent exocytosis of internalized receptor. It is proposed that the exocytosis of the approximately 50-nm bodies represents the mechanism by which the transferrin receptor is shed during reticulocyte maturation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Brown, K., Sacks, S. H., Wong, W. (2008). Extensive and bidirectional transfer of major histocompatibility complex class II molecules between donor and recipient cells in vivo following solid organ transplantation. FASEB J. 22: 3776-3784 [Abstract] [Full Text]
Obregon, C., Rothen-Rutishauser, B., Gitahi, S. K., Gehr, P., Nicod, L. P. (2006). Exovesicles from Human Activated Dendritic Cells Fuse with Resting Dendritic Cells, Allowing Them to Present Alloantigens. Am. J. Pathol. 169: 2127-2136 [Abstract] [Full Text]
van Niel, G., Porto-Carreiro, I., Simoes, S., Raposo, G. (2006). Exosomes: a common pathway for a specialized function.. J Biochem 140: 13-21 [Abstract] [Full Text]
Beatty, W. L. (2006). Trafficking from CD63-positive late endocytic multivesicular bodies is essential for intracellular development of Chlamydia trachomatis. J. Cell Sci. 119: 350-359 [Abstract] [Full Text]
Fevrier, B., Vilette, D., Archer, F., Loew, D., Faigle, W., Vidal, M., Laude, H., Raposo, G. (2004). Cells release prions in association with exosomes. Proc. Natl. Acad. Sci. USA 101: 9683-9688 [Abstract] [Full Text]
Andre, F., Chaput, N., Schartz, N. E. C., Flament, C., Aubert, N., Bernard, J., Lemonnier, F., Raposo, G., Escudier, B., Hsu, D.-H., Tursz, T., Amigorena, S., Angevin, E., Zitvogel, L. (2004). Exosomes as Potent Cell-Free Peptide-Based Vaccine. I. Dendritic Cell-Derived Exosomes Transfer Functional MHC Class I/Peptide Complexes to Dendritic Cells. J. Immunol. 172: 2126-2136 [Abstract] [Full Text]
Admyre, C., Grunewald, J., Thyberg, J., Gripenback, S., Tornling, G., Eklund, A., Scheynius, A., Gabrielsson, S. (2003). Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. Eur Respir J 22: 578-583 [Abstract] [Full Text]
Matsumoto, M., Funami, K., Tanabe, M., Oshiumi, H., Shingai, M., Seto, Y., Yamamoto, A., Seya, T. (2003). Subcellular Localization of Toll-Like Receptor 3 in Human Dendritic Cells. J. Immunol. 171: 3154-3162 [Abstract] [Full Text]
Wubbolts, R., Leckie, R. S., Veenhuizen, P. T. M., Schwarzmann, G., Mobius, W., Hoernschemeyer, J., Slot, J.-W., Geuze, H. J., Stoorvogel, W. (2003). Proteomic and Biochemical Analyses of Human B Cell-derived Exosomes. POTENTIAL IMPLICATIONS FOR THEIR FUNCTION AND MULTIVESICULAR BODY FORMATION. J. Biol. Chem. 278: 10963-10972 [Abstract] [Full Text]
Blanchard, N., Lankar, D., Faure, F., Regnault, A., Dumont, C., Raposo, G., Hivroz, C. (2002). TCR Activation of Human T Cells Induces the Production of Exosomes Bearing the TCR/CD3/{zeta} Complex. J. Immunol. 168: 3235-3241 [Abstract] [Full Text]
Kleijmeer, M., Ramm, G., Schuurhuis, D., Griffith, J., Rescigno, M., Ricciardi-Castagnoli, P., Rudensky, A. Y., Ossendorp, F., Melief, C. J.M., Stoorvogel, W., Geuze, H. J. (2001). Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. JCB 155: 53-64 [Abstract] [Full Text]
Thery, C., Boussac, M., Veron, P., Ricciardi-Castagnoli, P., Raposo, G., Garin, J., Amigorena, S. (2001). Proteomic Analysis of Dendritic Cell-Derived Exosomes: A Secreted Subcellular Compartment Distinct from Apoptotic Vesicles. J. Immunol. 166: 7309-7318 [Abstract] [Full Text]
Denzer, K, Kleijmeer, M., Heijnen, H., Stoorvogel, W, Geuze, H. (2000). Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J. Cell Sci. 113: 3365-3374 [Abstract]
Thery, C., Regnault, A., Garin, J., Wolfers, J., Zitvogel, L., Ricciardi-Castagnoli, P., Raposo, G., Amigorena, S. (1999). Molecular Characterization of Dendritic Cell-derived Exosomes: Selective Accumulation of the Heat Shock Protein hsc73. JCB 147: 599-610 [Abstract] [Full Text]
Garoff, H., Hewson, R., Opstelten, D.-J. E. (1998). Virus Maturation by Budding. Microbiol. Mol. Biol. Rev. 62: 1171-1190 [Abstract] [Full Text]
Albanese, J., Meterissian, S., Kontogiannea, M., Dubreuil, C., Hand, A., Sorba, S., Dainiak, N. (1998). Biologically Active Fas Antigen and Its Cognate Ligand Are Expressed on Plasma Membrane-Derived Extracellular Vesicles. Blood 91: 3862-3874 [Abstract] [Full Text]
Raposo, G., Tenza, D., Mecheri, S., Peronet, R., Bonnerot, C., Desaymard, C. (1997). Accumulation of Major Histocompatibility Complex Class II Molecules in Mast Cell Secretory Granules and Their Release upon Degranulation. Mol. Biol. Cell 8: 2631-2645 [Abstract] [Full Text]
Vidal, M, Mangeat, P, Hoekstra, D (1997). Aggregation reroutes molecules from a recycling to a vesicle-mediated secretion pathway during reticulocyte maturation. J. Cell Sci. 110: 1867-1877 [Abstract]
Ponka, P (1997). Tissue-specific regulation of iron metabolism and heme synthesis: distinct control mechanisms in erythroid cells. Blood 89: 1-25 [Abstract] [Full Text]
Geminard, C., Nault, F., Johnstone, R. M., Vidal, M. (2001). Characteristics of the Interaction between Hsc70 and the Transferrin Receptor in Exosomes Released during Reticulocyte Maturation. J. Biol. Chem. 276: 9910-9916 [Abstract] [Full Text]