Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane

doi:10.1083/jcb.101.3.949

This Article

	Full Text (PDF, 2346K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Griffiths, G.
	Articles by Matlin, K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Griffiths, G.
	Articles by Matlin, K.


Social Bookmarking

	What's this?

ARTICLES

Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane

G Griffiths, S Pfeiffer, K Simons and K Matlin

The intracellular location at which the G protein of vesicular stomatitis virus accumulated when transport was blocked at 20 degrees C has been studied by biochemical, cytochemical, and immunocytochemical methods. Our results indicated that the viral G protein was blocked in that cisterna of the Golgi stack which stained for acid phosphatase. At 20 degrees C this trans cisterna became structurally altered by the accumulation of G protein. This alteration was characterized by extensive areas of membrane buds which were covered by a cytoplasmic coat. These coated structures were of two kinds--those that labeled with anti-clathrin antibodies and those that did not. The clathrin- coated pits consistently did not label with anti-G antibodies. Upon warming infected cells to 32 degrees C, G protein appeared on the surface within minutes. Concomitantly, the trans cisterna lost its characteristic structural organization. Double-labeling experiments were performed in which G protein localization was combined with staining for horseradish peroxidase, which had been taken up from the extracellular medium by endocytosis. The results suggest that the trans cisterna was distinct from the endosome compartment and that the latter was not an obligatory station in the route taken by G protein to the cell surface.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Reiterer, V., Maier, S., Sitte, H. H., Kriz, A., Ruegg, M. A., Hauri, H.-P., Freissmuth, M., Farhan, H. (2008). Sec24- and ARFGAP1-Dependent Trafficking of GABA Transporter-1 Is a Prerequisite for Correct Axonal Targeting. J. Neurosci. 28: 12453-12464 [Abstract] [Full Text]
Chemin, J., Mezghrani, A., Bidaud, I., Dupasquier, S., Marger, F., Barrere, C., Nargeot, J., Lory, P. (2007). Temperature-dependent Modulation of CaV3 T-type Calcium Channels by Protein Kinases C and A in Mammalian Cells. J. Biol. Chem. 282: 32710-32718 [Abstract] [Full Text]
Sobota, J. A., Ferraro, F., Back, N., Eipper, B. A., Mains, R. E. (2006). Not All Secretory Granules Are Created Equal: Partitioning of Soluble Content Proteins. Mol. Biol. Cell 17: 5038-5052 [Abstract] [Full Text]
Kessels, M. M., Dong, J., Leibig, W., Westermann, P., Qualmann, B. (2006). Complexes of syndapin II with dynamin II promote vesicle formation at the trans-Golgi network. J. Cell Sci. 119: 1504-1516 [Abstract] [Full Text]
Hua, W., Sheff, D., Toomre, D., Mellman, I. (2006). Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.. JCB 172: 1035-1044 [Abstract] [Full Text]
Hasdemir, B., Fitzgerald, D. J., Prior, I. A., Tepikin, A. V., Burgoyne, R. D. (2005). Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway. JCB 171: 459-469 [Abstract] [Full Text]
Turcotte, S., Letellier, J., Lippe, R. (2005). Herpes Simplex Virus Type 1 Capsids Transit by the trans-Golgi Network, Where Viral Glycoproteins Accumulate Independently of Capsid Egress. J. Virol. 79: 8847-8860 [Abstract] [Full Text]
van Dam, E. M., Govers, R., James, D. E. (2005). Akt Activation Is Required at a Late Stage of Insulin-Induced GLUT4 Translocation to the Plasma Membrane. Mol. Endocrinol. 19: 1067-1077 [Abstract] [Full Text]
Atiya-Nasagi, Y., Cohen, H., Medalia, O., Fukudan, M., Sagi-Eisenberg, R. (2005). O-glycosylation is essential for intracellular targeting of synaptotagmins I and II in non-neuronal specialized secretory cells. J. Cell Sci. 118: 1363-1372 [Abstract] [Full Text]
Amarilio, R., Ramachandran, S., Sabanay, H., Lev, S. (2005). Differential Regulation of Endoplasmic Reticulum Structure through VAP-Nir Protein Interaction. J. Biol. Chem. 280: 5934-5944 [Abstract] [Full Text]
Haynes, L. P., Thomas, G. M. H., Burgoyne, R. D. (2005). Interaction of Neuronal Calcium Sensor-1 and ADP-ribosylation Factor 1 Allows Bidirectional Control of Phosphatidylinositol 4-Kinase {beta} and trans-Golgi Network-Plasma Membrane Traffic. J. Biol. Chem. 280: 6047-6054 [Abstract] [Full Text]
Mazzone, M., Baldassarre, M., Beznoussenko, G., Giacchetti, G., Cao, J., Zucker, S., Luini, A., Buccione, R. (2004). Intracellular processing and activation of membrane type 1 matrix metalloprotease depends on its partitioning into lipid domains. J. Cell Sci. 117: 6275-6287 [Abstract] [Full Text]
Ang, A. L., Taguchi, T., Francis, S., Folsch, H., Murrells, L. J., Pypaert, M., Warren, G., Mellman, I. (2004). Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells. JCB 167: 531-543 [Abstract] [Full Text]
Wagner, C. A., Finberg, K. E., Breton, S., Marshansky, V., Brown, D., Geibel, J. P. (2004). Renal Vacuolar H+-ATPase. Physiol. Rev. 84: 1263-1314 [Abstract] [Full Text]
Pager, C. T., Wurth, M. A., Dutch, R. E. (2004). Subcellular Localization and Calcium and pH Requirements for Proteolytic Processing of the Hendra Virus Fusion Protein. J. Virol. 78: 9154-9163 [Abstract] [Full Text]
Stockli, J., Rohrer, J. (2004). The Palmitoyltransferase of the Cation-dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes. Mol. Biol. Cell 15: 2617-2626 [Abstract] [Full Text]
Hanus, C., Vannier, C., Triller, A. (2004). Intracellular Association of Glycine Receptor with Gephyrin Increases Its Plasma Membrane Accumulation Rate. J. Neurosci. 24: 1119-1128 [Abstract] [Full Text]
Bard, F., Mazelin, L., Pechoux-Longin, C., Malhotra, V., Jurdic, P. (2003). Src Regulates Golgi Structure and KDEL Receptor-dependent Retrograde Transport to the Endoplasmic Reticulum. J. Biol. Chem. 278: 46601-46606 [Abstract] [Full Text]
Polishchuk, E. V., Di Pentima, A., Luini, A., Polishchuk, R. S. (2003). Mechanism of Constitutive Export from the Golgi: Bulk Flow via the Formation, Protrusion, and En Bloc Cleavage of large trans-Golgi Network Tubular Domains. Mol. Biol. Cell 14: 4470-4485 [Abstract] [Full Text]
Folsch, H., Pypaert, M., Maday, S., Pelletier, L., Mellman, I. (2003). The AP-1A and AP-1B clathrin adaptor complexes define biochemically and functionally distinct membrane domains. JCB 163: 351-362 [Abstract] [Full Text]
Nyasae, L. K., Hubbard, A. L., Tuma, P. L. (2003). Transcytotic Efflux from Early Endosomes Is Dependent on Cholesterol and Glycosphingolipids in Polarized Hepatic Cells. Mol. Biol. Cell 14: 2689-2705 [Abstract] [Full Text]
Puertollano, R., van der Wel, N. N., Greene, L. E., Eisenberg, E., Peters, P. J., Bonifacino, J. S. (2003). Morphology and Dynamics of Clathrin/GGA1-coated Carriers Budding from the Trans-Golgi Network. Mol. Biol. Cell 14: 1545-1557 [Abstract] [Full Text]
Wu, X., Zhao, X., Puertollano, R., Bonifacino, J. S., Eisenberg, E., Greene, L. E. (2003). Adaptor and Clathrin Exchange at the Plasma Membrane and trans-Golgi Network. Mol. Biol. Cell 14: 516-528 [Abstract] [Full Text]
Baldwin, T. A., Ostergaard, H. L. (2002). The Protein-tyrosine Phosphatase CD45 Reaches the Cell Surface via Golgi-dependent and -independent Pathways. J. Biol. Chem. 277: 50333-50340 [Abstract] [Full Text]
Zhu, Y. L., Conway-Campbell, B., Waters, M. J., Dannies, P. S. (2002). Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II. Endocrinology 143: 4243-4248 [Abstract] [Full Text]
Ladinsky, M. S., Wu, C. C., McIntosh, S., McIntosh, J. R., Howell, K. E. (2002). Structure of the Golgi and Distribution of Reporter Molecules at 20{degrees}C Reveals the Complexity of the Exit Compartments. Mol. Biol. Cell 13: 2810-2825 [Abstract] [Full Text]
Luo, C., Nobusawa, E., Nakajima, K. (2002). Analysis of the desialidation process of the haemagglutinin protein of influenza B virus: the host-dependent desialidation step. J. Gen. Virol. 83: 1729-1734 [Abstract] [Full Text]
Nishimura, N., Plutner, H., Hahn, K., Balch, W. E. (2002). The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface. Proc. Natl. Acad. Sci. USA 99: 6755-6760 [Abstract] [Full Text]
Rosenberg, M., Meier, J., Triller, A., Vannier, C. (2001). Dynamics of Glycine Receptor Insertion in the Neuronal Plasma Membrane. J. Neurosci. 21: 5036-5044 [Abstract] [Full Text]
Folsch, H., Pypaert, M., Schu, P., Mellman, I. (2001). Distribution and Function of AP-1 Clathrin Adaptor Complexes in Polarized Epithelial Cells. JCB 152: 595-606 [Abstract] [Full Text]
Weise, F, Stierhof, Y., Kuhn, C, Wiese, M, Overath, P (2000). Distribution of GPI-anchored proteins in the protozoan parasite Leishmania, based on an improved ultrastructural description using high-pressure frozen cells. J. Cell Sci. 113: 4587-4603 [Abstract]
Polishchuk, R. S., Polishchuk, E. V., Marra, P., Alberti, S., Buccione, R., Luini, A., Mironov, A. A. (2000). Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane. JCB 148: 45-58 [Abstract] [Full Text]
Baldassarre, M, Dragonetti, A, Marra, P, Luini, A, Isidoro, C, Buccione, R (2000). Regulation of protein sorting at the TGN by plasma membrane receptor activation. J. Cell Sci. 113: 741-748 [Abstract]
Valetti, C., Wetzel, D. M., Schrader, M., Hasbani, M. J., Gill, S. R., Kreis, T. E., Schroer, T. A. (1999). Role of Dynactin in Endocytic Traffic: Effects of Dynamitin Overexpression and Colocalization with CLIP-170. Mol. Biol. Cell 10: 4107-4120 [Abstract] [Full Text]
Auger, R., Robin, P., Camier, B., Vial, G., Rossignol, B., Tenu, J.-P., Raymond, M.-N. (1999). Relationship between Phosphatidic Acid Level and Regulation of Protein Transit in Colonic Epithelial Cell Line HT29-cl19A. J. Biol. Chem. 274: 28652-28659 [Abstract] [Full Text]
Zheng, X., Lu, D., Sadler, J. E. (1999). Apical Sorting of Bovine Enteropeptidase Does Not Involve Detergent-resistant Association with Sphingolipid-Cholesterol Rafts. J. Biol. Chem. 274: 1596-1605 [Abstract] [Full Text]
Sai, Y, Nies, A., Arias, I. (1999). Bile acid secretion and direct targeting of mdr1-green fluorescent protein from Golgi to the canalicular membrane in polarized WIF-B cells. J. Cell Sci. 112: 4535-4545 [Abstract]
Toomre, D, Keller, P, White, J, Olivo, J., Simons, K (1999). Dual-color visualization of trans-Golgi network to plasma membrane traffic along microtubules in living cells. J. Cell Sci. 112: 21-33 [Abstract]
Prescott, J. L., Tindall, D. J. (1998). Clathrin Gene Expression Is Androgen Regulated in the Prostate. Endocrinology 139: 2111-2119 [Abstract] [Full Text]
Rahkila, P., Luukela, V., Vaananen, K., Metsikko, K. (1998). Differential Targeting of Vesicular Stomatitis Virus G Protein and Influenza Virus Hemagglutinin Appears During Myogenesis of L6 Muscle Cells. JCB 140: 1101-1111 [Abstract] [Full Text]
Pepperkok, R, Lowe, M, Burke, B, Kreis, T. (1998). Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S. J. Cell Sci. 111: 1877-1888 [Abstract]
Drecktrah, D, de Figueiredo, P, Mason, R., Brown, W. (1998). Retrograde trafficking of both Golgi complex and TGN markers to the ER induced by nordihydroguaiaretic acid and cyclofenil diphenol. J. Cell Sci. 111: 951-965 [Abstract]
Robin, P., Rossignol, B., Raymond, M. N. (1998). PKA inhibitor, H-89, affects the intracellular transit of regulated secretory proteins in rat lacrimal glands. Am. J. Physiol. Cell Physiol. 274: C262-C271 [Abstract] [Full Text]
Wolins, N., Bosshart, H., Kuster, H., Bonifacino, J. S. (1997). Aggregation As a Determinant of Protein Fate in Post-Golgi Compartments: Role of the Luminal Domain of Furin in Lysosomal Targeting. JCB 139: 1735-1745 [Abstract] [Full Text]
Muniz, M., Martin, M. E., Hidalgo, J., Velasco, A. (1997). Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi�network. Proc. Natl. Acad. Sci. USA 94: 14461-14466 [Abstract] [Full Text]
Marx, R., Mains, R. E. (1997). Adenovirally Encoded Prohormone Convertase-1 Functions in Atrial Myocyte Large Dense Core Vesicles. Endocrinology 138: 5108-5118 [Abstract] [Full Text]
Musch, A., Cohen, D., Rodriguez-Boulan, E. (1997). Myosin II Is Involved in the Production of Constitutive Transport Vesicles from the TGN. JCB 138: 291-306 [Abstract] [Full Text]
Scheel, J., Pepperkok, R., Lowe, M., Griffiths, G., Kreis, T. E. (1997). Dissociation of Coatomer from Membranes Is Required for Brefeldin A-induced Transfer of Golgi Enzymes to the Endoplasmic Reticulum. JCB 137: 319-333 [Abstract] [Full Text]
Muller, L., Barret, A., Picart, R., Tougard, C. (1997). Proteolytic Processing of Sulfated Secretogranin II in the trans-Golgi Network of GH3B6 Prolactin Cells. J. Biol. Chem. 272: 3669-3673 [Abstract] [Full Text]
Ma, J., Qian, R., Rausa, F. M. III, Colley, K. J. (1997). Two Naturally Occurring alpha 2,6-Sialyltransferase Forms with a Single Amino Acid Change in the Catalytic Domain Differ in Their Catalytic Activity and Proteolytic Processing. J. Biol. Chem. 272: 672-679 [Abstract] [Full Text]
Milgram, S., Kho, S., Martin, G., Mains, R., Eipper, B. (1997). Localization of integral membrane peptidylglycine alpha-amidating monooxygenase in neuroendocrine cells. J. Cell Sci. 110: 695-706 [Abstract]
Brunner, C., Lobentanz, E.-M., Petho-Schramm, A., Ernst, A., Kang, C., Dieplinger, H., Muller, H.-J., Utermann, G. (1996). The Number of Identical Kringle IV Repeats in Apolipoprotein(a) Affects Its Processing and Secretion by HepG2 Cells. J. Biol. Chem. 271: 32403-32410 [Abstract] [Full Text]
Muniz, M., Alonso, M., Hidalgo, J., Velasco, A. (1996). A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route. J. Biol. Chem. 271: 30935-30941 [Abstract] [Full Text]
Cruz, I. P. d. l., Nillni, E. A. (1996). Intracellular Sites of Prothyrotropin-releasing Hormone Processing. J. Biol. Chem. 271: 22736-22745 [Abstract] [Full Text]
Rahkila, P, Alakangas, A, Vaananen, K, Metsikko, K (1996). Transport pathway, maturation, and targetting of the vesicular stomatitis virus glycoprotein in skeletal muscle fibers. J. Cell Sci. 109: 1585-1596 [Abstract]
Beldent, V�r., Michaud, A., Bonnefoy, C., Chauvet, M.-T.�r�s., Corvol, P. (1995). Cell Surface Localization of Proteolysis of Human Endothelial Angiotensin I-converting Enzyme. J. Biol. Chem. 270: 28962-28969 [Abstract] [Full Text]
Locker, J. K., Opstelten, D.-J. E., Ericsson, M., Horzinek, M. C., Rottier, P. J. M. (1995). Oligomerization of a trans-Golgi/ trans-Golgi Network Retained Protein Occurs in the Golgi Complex and May Be Part of Its Retention. J. Biol. Chem. 270: 8815-8821 [Abstract] [Full Text]
Griffiths, G, Pepperkok, R, Locker, J., Kreis, T. (1995). Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN. J. Cell Sci. 108: 2839-2856 [Abstract]
Sariola, M, Saraste, J, Kuismanen, E (1995). Communication of post-Golgi elements with early endocytic pathway: regulation of endoproteolytic cleavage of Semliki Forest virus p62 precursor. J. Cell Sci. 108: 2465-2475 [Abstract]
Brignoni, M, Pignataro, O., Rodriguez, M., Alvarez, A, Vega-Salas, D., Rodriguez-Boulan, E, Salas, P. (1995). Cyclic AMP modulates the rate of 'constitutive' exocytosis of apical membrane proteins in Madin-Darby canine kidney cells. J. Cell Sci. 108: 1931-1943 [Abstract]
Buxbaum, E, Woodman, P. (1995). Selective action of uncoating ATPase towards clathrin-coated vesicles from brain. J. Cell Sci. 108: 1295-1306 [Abstract]
Wang, J., Ladinsky, M.S., Howell, K.E. (1995). Molecules and Vesicle Coats Involved in the Budding of Exocytotic Vesicles from the trans-Golgi Network. Cold Spring Harb Symp Quant Biol 60: 139-146 [Abstract]
Simon, J.-P., Ivanov, I.E., Ren, M., Zeng, J., Shopsin, B., Hersh, D., Tempst, P., Erdjument-Bromage, H., Lui, M., De Lemos-Chiarandini, C., Rosenfeld, M., Gravotta, D., Morimoto, T., Adesnik, M., Sabatini, D.D. (1995). Regulation of Post-Golgi Vesicle Production in an In Vitro System. Cold Spring Harb Symp Quant Biol 60: 179-195 [Abstract]
Oyarce, A., Eipper, B. (1995). Identification of subcellular compartments containing peptidylglycine alpha-amidating monooxygenase in rat anterior pituitary. J. Cell Sci. 108: 287-297 [Abstract]
Lutcke, A, Parton, R., Murphy, C, Olkkonen, V., Dupree, P, Valencia, A, Simons, K, Zerial, M (1994). Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression. J. Cell Sci. 107: 3437-3448 [Abstract]
Milgram, S., Mains, R. (1994). Differential effects of temperature blockade on the proteolytic processing of three secretory granule-associated proteins. J. Cell Sci. 107: 737-745 [Abstract]
Olkkonen, V., Dupree, P, Killisch, I, Lutcke, A, Zerial, M, Simons, K (1993). Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. J. Cell Sci. 106: 1249-1261 [Abstract]
Rudick, V., Rudick, M., Brun-Zinkernagel, A. (1993). Amplification of the Golgi complex in MDCK cells secreting human growth hormone. J. Cell Sci. 104: 509-520 [Abstract]
Lucocq, J. (1992). Mimicking mitotic Golgi disassembly using okadaic acid. J. Cell Sci. 103: 875-880
Antony, C., Cibert, C., Geraud, G., Santa Maria, A., Maro, B., Mayau, V., Goud, B. (1992). The small GTP-binding protein rab6p is distributed from medial Golgi to the trans-Golgi network as determined by a confocal microscopic approach. J. Cell Sci. 103: 785-796 [Abstract]
Griffiths, G, Simons, K (1986). The trans Golgi network: sorting at the exit site of the Golgi complex. Science 234: 438-443 [Abstract]
Payne, G., Schekman, R (1985). A test of clathrin function in protein secretion and cell growth. Science 230: 1009-1014 [Abstract]
Baron, C. L., Malhotra, V. (2002). Role of Diacylglycerol in PKD Recruitment to the TGN and Protein Transport to the Plasma Membrane. Science 295: 325-328 [Abstract] [Full Text]
Lee, M. S., Zhu, Y. L., Chang, J. E., Dannies, P. S. (2001). Acquisition of Lubrol Insolubility, a Common Step for Growth Hormone and Prolactin in the Secretory Pathway of Neuroendocrine Cells. J. Biol. Chem. 276: 715-721 [Abstract] [Full Text]