A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation

doi:10.1083/jcb.101.5.1903

This Article

Full Text (PDF, 1402K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Wright, R. L.

Articles by Jarvik, J. W.

Search for Related Content

PubMed

PubMed Citation

Articles by Wright, R. L.

Articles by Jarvik, J. W.

Pubmed/NCBI databases

Hazardous Substances DB
Compound via MeSH
Substance via MeSH
CALCIUM COMPOUNDS
CALCIUM, ELEMENTAL

Social Bookmarking

What's this?

ARTICLES

A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation

RL Wright, J Salisbury and JW Jarvik

We have isolated a nucleus-basal body complex from Chlamydomonas reinhardtii. The complex is strongly immunoreactive to an antibody generated against a major protein constituent of isolated Tetraselmis striata flagellar roots (Salisbury, J. L., A. Baron, B. Surek, and M. Melkonian, J. Cell Biol., 99:962-970). Electrophoretic and immunoelectrophoretic analysis indicates that, like the Tetraselmis protein, the Chlamydomonas antigen consists of two acidic isoforms of approximately 20 kD. Indirect immunofluorescent staining of nucleus- basal body complexes reveals two major fibers in the connector region, one between each basal body and the nucleus. The nucleus is also strongly immunoreactive, with staining radiating around much of the nucleus from a region of greatest concentration at the connector pole. Calcium treatment causes shortening of the connector fibers and also movement of nuclear DNA towards the connector pole. Electron microscopic observation of negatively stained nucleus-basal body complexes reveals a cluster of approximately 6-nm filaments, suspected to represent the connector, between the basal bodies and nuclei. A mutant with a variable number of flagella, vfl-2-220, is defective with respect to the nucleus-basal body association. This observation encourages us to speculate that the nucleus-basal body union is important for accurate basal body localization within the cell and/or for accurate segregation of parental and daughter basal bodies at cell division. A physical association between nuclei and basal bodies or centrioles has been observed in a variety of algal, protozoan, and metazoan cells, although the nature of the association, in terms of both structure and function, has been obscure. We believe it likely that fibrous connectors homologous to those described here for Chlamydomonas are general features of centriole-bearing eucaryotic cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Mahajan, B., Selvapandiyan, A., Gerald, N. J., Majam, V., Zheng, H., Wickramarachchi, T., Tiwari, J., Fujioka, H., Moch, J. K., Kumar, N., Aravind, L., Nakhasi, H. L., Kumar, S. (2008). Centrins, Cell Cycle Regulation Proteins in Human Malaria Parasite Plasmodium falciparum. J. Biol. Chem. 283: 31871-31883 [Abstract] [Full Text]
Gogendeau, D., Klotz, C., Arnaiz, O., Malinowska, A., Dadlez, M., de Loubresse, N. G., Ruiz, F., Koll, F., Beisson, J. (2008). Functional diversification of centrins and cell morphological complexity. J. Cell Sci. 121: 65-74 [Abstract] [Full Text]
Yamamoto, R., Yanagisawa, H.-a., Yagi, T., Kamiya, R. (2008). Novel 44-Kilodalton Subunit of Axonemal Dynein Conserved from Chlamydomonas to Mammals. Eukaryot Cell 7: 154-161 [Abstract] [Full Text]
Marshall, W. F. (2007). Stability and Robustness of an Organelle Number Control System: Modeling and Measuring Homeostatic Regulation of Centriole Abundance. Biophys. J 93: 1818-1833 [Abstract] [Full Text]
Selvapandiyan, A., Kumar, P., Morris, J. C., Salisbury, J. L., Wang, C. C., Nakhasi, H. L. (2007). Centrin1 Is Required for Organelle Segregation and Cytokinesis in Trypanosoma brucei. Mol. Biol. Cell 18: 3290-3301 [Abstract] [Full Text]
Sagolla, M. S., Dawson, S. C., Mancuso, J. J., Cande, W. Z. (2006). Three-dimensional analysis of mitosis and cytokinesis in the binucleate parasite Giardia intestinalis. J. Cell Sci. 119: 4889-4900 [Abstract] [Full Text]
Dymek, E. E., Goduti, D., Kramer, T., Smith, E. F. (2006). A kinesin-like calmodulin-binding protein in Chlamydomonas: evidence for a role in cell division and flagellar functions. J. Cell Sci. 119: 3107-3116 [Abstract] [Full Text]
Stemm-Wolf, A. J., Morgan, G., Giddings, T. H. Jr., White, E. A., Marchione, R., McDonald, H. B., Winey, M. (2005). Basal Body Duplication and Maintenance Require One Member of the Tetrahymena thermophila Centrin Gene Family. Mol. Biol. Cell 16: 3606-3619 [Abstract] [Full Text]
Geimer, S., Melkonian, M. (2005). Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy. Eukaryot Cell 4: 1253-1263 [Abstract] [Full Text]
Dymek, E. E., Lefebvre, P. A., Smith, E. F. (2004). PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules. Eukaryot Cell 3: 870-879 [Abstract] [Full Text]
Matsuura, K., Lefebvre, P. A., Kamiya, R., Hirono, M. (2004). Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly. JCB 165: 663-671 [Abstract] [Full Text]
Geimer, S., Melkonian, M. (2004). The ultrastructure of the Chlamydomonas reinhardtii basal apparatus: identification of an early marker of radial asymmetry inherent in the basal body. J. Cell Sci. 117: 2663-2674 [Abstract] [Full Text]
O'Toole, E. T., Giddings, T. H., McIntosh, J. R., Dutcher, S. K. (2003). Three-dimensional Organization of Basal Bodies from Wild-Type and {delta}-Tubulin Deletion Strains of Chlamydomonas reinhardtii. Mol. Biol. Cell 14: 2999-3012 [Abstract] [Full Text]
Hirono, M., Uryu, S., Ohara, A., Kato-Minoura, T., Kamiya, R. (2003). Expression of Conventional and Unconventional Actins in Chlamydomonas reinhardtii upon Deflagellation and Sexual Adhesion. Eukaryot Cell 2: 486-493 [Abstract] [Full Text]
Pfannenschmid, F., Wimmer, V. C., Rios, R.-M., Geimer, S., Krockel, U., Leiherer, A., Haller, K., Nemcova, Y., Mages, W. (2003). Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division. J. Cell Sci. 116: 1449-1462 [Abstract] [Full Text]
Ikeda, K., Brown, J. A., Yagi, T., Norrander, J. M., Hirono, M., Eccleston, E., Kamiya, R., Linck, R. W. (2003). Rib72, a Conserved Protein Associated with the Ribbon Compartment of Flagellar A-microtubules and Potentially Involved in the Linkage between Outer Doublet Microtubules. J. Biol. Chem. 278: 7725-7734 [Abstract] [Full Text]
Silflow, C. D., LaVoie, M., Tam, L.-W., Tousey, S., Sanders, M., Wu, W.-c., Borodovsky, M., Lefebvre, P. A. (2001). The Vfl1 Protein in Chlamydomonas Localizes in a Rotationally Asymmetric Pattern at the Distal Ends of the Basal Bodies. JCB 153: 63-74 [Abstract] [Full Text]
Ivanovska, I., Rose, M. D. (2001). Fine Structure Analysis of the Yeast Centrin, Cdc31p, Identifies Residues Specific for Cell Morphology and Spindle Pole Body Duplication. Genetics 157: 503-518 [Abstract] [Full Text]
Preble, A. M., Giddings, T. H. , Jr., Dutcher, S. K. (2001). Extragenic Bypass Suppressors of Mutations in the Essential Gene BLD2 Promote Assembly of Basal Bodies With Abnormal Microtubules in Chlamydomonas reinhardtii. Genetics 157: 163-181 [Abstract] [Full Text]
Middendorp, S., Kuntziger, T., Abraham, Y., Holmes, S., Bordes, N., Paintrand, M., Paoletti, A., Bornens, M. (2000). A Role for Centrin 3 in Centrosome Reproduction. JCB 148: 405-416 [Abstract] [Full Text]
Laoukili, J, Perret, E, Middendorp, S, Houcine, O, Guennou, C, Marano, F, Bornens, M, Tournier, F (2000). Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro. J. Cell Sci. 113: 1355-1364 [Abstract]
Norrander, J. M., deCathelineau, A. M., Brown, J. A., Porter, M. E., Linck, R. W. (2000). The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas. Mol. Biol. Cell 11: 201-215 [Abstract] [Full Text]
Middendorp, S., Paoletti, A., Schiebel, E., Bornens, M. (1997). Identification of a new mammalian centrin gene, more closely related to Saccharomyces cerevisiae CDC31�gene. Proc. Natl. Acad. Sci. USA 94: 9141-9146 [Abstract] [Full Text]
Geier, B. M., Wiech, H., Schiebel, E. (1996). Binding of Centrins and Yeast Calmodulin to Synthetic Peptides Corresponding to Binding Sites in the Spindle Pole Body Components Kar1p and Spc110p. J. Biol. Chem. 271: 28366-28374 [Abstract] [Full Text]
Wiech, H., Geier, B. M., Paschke, T., Spang, A., Grein, K., Steinkotter, J., Melkonian, M., Schiebel, E. (1996). Characterization of Green Alga, Yeast, and Human Centrins. SPECIFIC SUBDOMAIN FEATURES DETERMINE FUNCTIONAL DIVERSITY. J. Biol. Chem. 271: 22453-22461 [Abstract] [Full Text]
Paoletti, A, Moudjou, M, Paintrand, M, Salisbury, J., Bornens, M (1996). Most of centrin in animal cells is not centrosome-associated and centrosomal centrin is confined to the distal lumen of centrioles. J. Cell Sci. 109: 3089-3102 [Abstract]
Baron, A., Suman, V., Nemeth, E, Salisbury, J. (1994). The pericentriolar lattice of PtK2 cells exhibits temperature and calcium-modulated behavior. J. Cell Sci. 107: 2993-3003 [Abstract]
Errabolu, R, Sanders, M., Salisbury, J. (1994). Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles. J. Cell Sci. 107: 9-16 [Abstract]