The Journal of Cell Biology, Vol 105, 335-344, Copyright © 1987 by The Rockefeller University Press

ARTICLES

Assembly of the intestinal brush border: appearance and redistribution of microvillar core proteins in developing chick enterocytes

T Shibayama, JM Carboni and MS Mooseker

The assembly of the intestinal microvillus cytoskeleton during embryogenesis in the chick was examined by immunochemical and light microscopic immunolocalization techniques. For these studies, affinity- purified antibodies reactive with three major cytoskeletal proteins of the adult intestinal microvillus, fimbrin, villin, and the 110-kD subunit of the 110K-calmodulin protein complex were prepared. Immunocytochemical staining of frozen sections of embryonic duodena revealed that all three proteins were present at detectable levels at the earliest stages examined, day 7-8 of incubation (Hamilton/Hamburger stages 25-30). Although initially all three proteins were diffusely distributed throughout the cytoplasm, there was a marked asynchrony in the accumulation of these core proteins within the apical domain of the enterocyte. Villin displayed concentrated apical staining by embryonic day 8 (stage 28), while the apical concentration of fimbrin was first observed at embryonic day 10 (stage 37). Diffuse staining of the enterocyte cytoplasm with the anti-110K was observed throughout development until a few days before hatch. By embryonic day 19-21 110K staining was concentrated at the cell periphery (apical and basolateral). The restricted apical localization characteristic of 110K in the adult brush border was not observed until the day of hatching. Immunoblot analysis of whole, solubilized embryonic duodena confirmed the presence of 110K, villin, and fimbrin throughout development and indicated substantial increases in all three proteins, particularly late in development. Immunoblot staining with anti-110K also revealed the presence of a high molecular mass (200 kD) immunoreactive species in embryonic intestine. This 200-kD form was absent from isolated embryonic enterocytes and may be a component of intestinal smooth muscle.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Delacour, D., Koch, A., Ackermann, W., Parco, I. E.-L., Elsasser, H.-P., Poirier, F., Jacob, R. (2008). Loss of galectin-3 impairs membrane polarisation of mouse enterocytes in vivo. J. Cell Sci. 121: 458-465 [Abstract] [Full Text]  
• George, S. P., Wang, Y., Mathew, S., Srinivasan, K., Khurana, S. (2007). Dimerization and Actin-bundling Properties of Villin and Its Role in the Assembly of Epithelial Cell Brush Borders. J. Biol. Chem. 282: 26528-26541 [Abstract] [Full Text]  
• DeRosier, D. J., Tilney, L. G. (2000). F-Actin Bundles Are Derivatives of Microvilli: What Does This Tell Us about How Bundles Might Form?. JCB 148: 1-6 [Abstract] [Full Text]  
• Li, W., Wang, J., Coluccio, L. M., Matsudaira, P., Grand, R. J. (2000). Brush Border Myosin I (BBMI): A Basally Localized Transcript in Human Jejunal Enterocytes. J. Histochem. Cytochem. 48: 89-94 [Abstract] [Full Text]  
• Matova, N, Mahajan-Miklos, S, Mooseker, M., Cooley, L (1999). Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells. Development 126: 5645-5657 [Abstract]  
• Tilney, L. G., Connelly, P. S., Vranich, K. A., Shaw, M. K., Guild, G. M. (1998). Why Are Two Different Cross-linkers Necessary for Actin Bundle Formation In Vivo and What Does Each Cross-link Contribute?. JCB 143: 121-133 [Abstract] [Full Text]  
• Cant, K, Knowles, B., Mahajan-Miklos, S, Heintzelman, M, Cooley, L (1998). Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail. J. Cell Sci. 111: 213-221 [Abstract]  
• Brown, D., Lee, R., Bonventre, J. V. (1997). Redistribution of villin to proximal tubule basolateral membranes after ischemia and reperfusion. Am. J. Physiol. Renal Physiol. 273: F1003-F1012 [Abstract] [Full Text]  
• Salas, P. J.I., Rodriguez, M. L., Viciana, A. L., Vega-Salas, D. E., Hauri, H.-P. (1997). The Apical Submembrane Cytoskeleton Participates in the Organization of the Apical Pole in Epithelial Cells. JCB 137: 359-375 [Abstract] [Full Text]  
• Fitzgerald, R., Omary, M., Triadafilopoulos, G (1997). Acid modulation of HT29 cell growth and differentiation. An in vitro model for Barrett's esophagus. J. Cell Sci. 110: 663-671 [Abstract]  
• Footer, M, Bretscher, A (1994). Brush border myosin-I microinjected into cultured cells is targeted to actin-containing surface structures. J. Cell Sci. 107: 1623-1631 [Abstract]  
• Berryman, M, Franck, Z, Bretscher, A (1993). Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells. J. Cell Sci. 105: 1025-1043 [Abstract]  
• Friederich, E, Kreis, T., Louvard, D (1993). Villin-induced growth of microvilli is reversibly inhibited by cytochalasin D. J. Cell Sci. 105: 765-775 [Abstract]  
• Peterson, M., Bement, W., Mooseker, M. (1993). An in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells. J. Cell Sci. 105: 461-472 [Abstract]  
• Heusser, S., Colin, S., Figiel, A., Huet, C., Keller, J. M., Pornet, P., Robine, S., Vandamme, J., Vandekerckhove, J., Dauca, M. (1992). Amphibian intestinal villin: isolation and expression during embryonic and larval development. J. Cell Sci. 103: 699-708 [Abstract]  
• Maunoury, R, Robine, S, Pringault, E, Leonard, N, Gaillard, J., Louvard, D (1992). Developmental regulation of villin gene expression in the epithelial cell lineages of mouse digestive and urogenital tracts. Development 115: 717-728 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents