The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines

doi:10.1083/jcb.105.2.791

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The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines

K Albers and E Fuchs

We have deleted cDNA sequences encoding portions of the carboxy- terminal end of a human type I epidermal keratin K14, and examined the molecular consequences of forcing the expression of these mutants in simple epithelial and squamous cell carcinoma lines. To follow the expression of our mutant products in transfected cells, we have tagged the 3' end of the K14 coding sequence with a sequence encoding an antigenic domain of the neuropeptide substance P. Using DNA transfection and immunohistochemistry (with an antibody against substance P), we have identified a collection of mutants that have a wide range of morphological effects on the endogenous keratin filament networks of transfected cells. Mutants that are missing most of the nonhelical carboxy-terminal domain of K14 incorporate into the endogenous keratin filaments without any visible perturbations on the network. In contrast, mutants that are missing as few as 10 of the 310 amino acids of the central alpha-helical domain of the polypeptide cause gross alterations in the keratin network. In some cases, the entire cytoskeletal network of keratins was disrupted, leaving no evidence of 8-nm filaments. These results reveal the existence of a dynamic exchange between newly synthesized subunits and preexisting keratin filaments.
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Shi, J., Sugrue, S. P. (2000). Dissection of Protein Linkage between Keratins and Pinin, a Protein with Dual Location at Desmosome-Intermediate Filament Complex and in the Nucleus. J. Biol. Chem. 275: 14910-14915 [Abstract] [Full Text]
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Inada, H., Togashi, H., Nakamura, Y., Kaibuchi, K., Nagata, K.-i., Inagaki, M. (1999). Balance between Activities of Rho Kinase and Type 1 Protein Phosphatase Modulates Turnover of Phosphorylation and Dynamics of Desmin/Vimentin Filaments. J. Biol. Chem. 274: 34932-34939 [Abstract] [Full Text]
Fradette, J., Germain, L., Seshaiah, P., Coulombe, P. A. (1998). The Type I Keratin 19�Possesses Distinct and Context-dependent Assembly Properties. J. Biol. Chem. 273: 35176-35184 [Abstract] [Full Text]
Porter, R. M., Hutcheson, A. M., Rugg, E. L., Quinlan, R. A., Lane, E. B. (1998). cDNA Cloning, Expression, and Assembly Characteristics of Mouse Keratin 16. J. Biol. Chem. 273: 32265-32272 [Abstract] [Full Text]
Rezniczek, G. A., de Pereda, J. M., Reipert, S., Wiche, G. (1998). Linking Integrin alpha 6beta 4-based Cell Adhesion to the Intermediate Filament Cytoskeleton: Direct Interaction between the beta 4 Subunit and Plectin at Multiple Molecular Sites. JCB 141: 209-225 [Abstract] [Full Text]
Magin, T. M., Schroder, R., Leitgeb, S., Wanninger, F., Zatloukal, K., Grund, C., Melton, D. W. (1998). Lessons from Keratin 18�Knockout Mice: Formation of Novel Keratin Filaments, Secondary Loss of Keratin 7�and Accumulation of Liver-specific Keratin 8-Positive Aggregates. JCB 140: 1441-1451 [Abstract] [Full Text]
Fuchs, E., Cleveland, D. W. (1998). A Structural Scaffolding of Intermediate Filaments in Health and Disease. Science 279: 514-519 [Abstract] [Full Text]
Winter, H., Hofmann, I., Langbein, L., Rogers, M. A., Schweizer, J. (1997). A Splice Site Mutation in the Gene of the Human Type I Hair Keratin hHa1 Results in the Expression of a Tailless Keratin Isoform. J. Biol. Chem. 272: 32345-32352 [Abstract] [Full Text]
Reichelt, J, Bauer, C, Porter, R, Lane, E, Magin, V (1997). Out of balance: consequences of a partial keratin 10 knockout. J. Cell Sci. 110: 2175-2186 [Abstract]
Wang, X., Zinkel, S., Polonsky, K., Fuchs, E. (1997). Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene�therapy. Proc. Natl. Acad. Sci. USA 94: 219-226 [Abstract] [Full Text]
Paramio, J., Casanova, M., Alonso, A, Jorcano, J. (1997). Keratin intermediate filament dynamics in cell heterokaryons reveals diverse behaviour of different keratins. J. Cell Sci. 110: 1099-1111 [Abstract]
Mukai, H., Toshimori, M., Shibata, H., Kitagawa, M., Shimakawa, M., Miyahara, M., Sunakawa, H., Ono, Y. (1996). PKN Associates and Phosphorylates the Head-Rod Domain of Neurofilament Protein. J. Biol. Chem. 271: 9816-9822 [Abstract] [Full Text]
Goulielmos, G, Remington, S, Schwesinger, F, Georgatos, S., Gounari, F (1996). Contributions of the structural domains of filensin in polymer formation and filament distribution. J. Cell Sci. 109: 447-456 [Abstract]
Garlick, J.A., Fenjves, E.S. (1996). Keratinocyte Gene Transfer and Gene Therapy. fake Crit Rev Oral Biol Med 7: 204-221 [Abstract] [Full Text]
Morley, S., Dundas, S., James, J., Gupta, T, Brown, R., Sexton, C., Navsaria, H., Leigh, I., Lane, E. (1995). Temperature sensitivity of the keratin cytoskeleton and delayed spreading of keratinocyte lines derived from EBS patients. J. Cell Sci. 108: 3463-3471 [Abstract]
Cui, C, Stambrook, P., Parysek, L. (1995). Peripherin assembles into homopolymers in SW13 cells. J. Cell Sci. 108: 3279-3284 [Abstract]
Cary, R., Klymkowsky, M. (1995). Disruption of intermediate filament organization leads to structural defects at the intersomite junction in Xenopus myotomal muscle. Development 121: 1041-1052 [Abstract]
Rugg, E L, McLean, W H, Lane, E B, Pitera, R, McMillan, J R, Dopping-Hepenstal, P J, Navsaria, H A, Leigh, I M, Eady, R A (1994). A functional "knockout" of human keratin 14.. Genes Dev. 8: 2563-2573 [Abstract]
Chan, Y, Anton-Lamprecht, I, Yu, Q C, Jackel, A, Zabel, B, Ernst, J P, Fuchs, E (1994). A human keratin 14 "knockout": the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein.. Genes Dev. 8: 2574-2587 [Abstract]
Chen, W., Liem, R. (1994). The endless story of the glial fibrillary acidic protein. J. Cell Sci. 107: 2299-2311 [Abstract]
Sarria, A., Lieber, J., Nordeen, S., Evans, R. (1994). The presence or absence of a vimentin-type intermediate filament network affects the shape of the nucleus in human SW-13 cells. J. Cell Sci. 107: 1593-1607 [Abstract]
Chan, Y., Yu, Q., LeBlanc-Straceski, J, Christiano, A, Pulkkinen, L, Kucherlapati, R., Uitto, J, Fuchs, E (1994). Mutations in the non-helical linker segment L1-2 of keratin 5 in patients with Weber-Cockayne epidermolysis bullosa simplex. J. Cell Sci. 107: 765-774 [Abstract]
Coleman, T. R., Lazarides, E. (1992). Continuous growth of vimentin filaments in mouse fibroblasts. J. Cell Sci. 103: 689-698 [Abstract]
Epstein, E. Jr (1992). Molecular genetics of epidermolysis bullosa. Science 256: 799-804 [Abstract]
Leask, A, Rosenberg, M, Vassar, R, Fuchs, E (1990). Regulation of a human epidermal keratin gene: sequences and nuclear factors involved in keratinocyte-specific transcription.. Genes Dev. 4: 1985-1998 [Abstract]
Dale, B. A., Salonen, J., Jones, A. H. (1990). New Approaches And Concepts in The Study of Differentiation of Oral Epithelia. fake Crit Rev Oral Biol Med 1: 167-190 [Abstract] [Full Text]
Boulay, A., Regnier, C. H., Anglard, P., Stoll, I., Tomasetto, C., Rio, M.-C. (2001). Transcription Regulation and Protein Subcellular Localization of the Truncated Basic Hair Keratin hHb1-Delta N in Human Breast Cancer Cells. J. Biol. Chem. 276: 22954-22964 [Abstract] [Full Text]
Bousquet, O., Ma, L., Yamada, S., Gu, C., Idei, T., Takahashi, K., Wirtz, D., Coulombe, P. A. (2001). The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro. JCB 155: 747-754 [Abstract] [Full Text]