Distribution and modulation of the cellular receptor for transforming growth factor-beta

doi:10.1083/jcb.105.2.965

PeproTech: Your source for Cell Biology Research Reagents

This Article

Full Text (PDF, 1203K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Wakefield, L. M.

Articles by Sporn, M. B.

Search for Related Content

PubMed

PubMed Citation

Articles by Wakefield, L. M.

Articles by Sporn, M. B.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Distribution and modulation of the cellular receptor for transforming growth factor-beta

LM Wakefield, DM Smith, T Masui, CC Harris and MB Sporn

Scatchard analyses of the binding of transforming growth factor-beta (TGF-beta) to a wide variety of different cell types in culture revealed the universal presence of high affinity (Kd = 1-60 pM) receptors for TGF-beta on every cell type assayed, indicating a wide potential target range for TGF-beta action. There was a strong (r = +0.85) inverse relationship between the receptor affinity and the number of receptors expressed per cell, such that at low TGF-beta concentrations, essentially all cells bound a similar number of TGF- beta molecules per cell. The binding of TGF-beta to various cell types was not altered by many agents that affect the cellular response to TGF- beta, suggesting that modulation of TGF-beta binding to its receptor may not be a primary control mechanism in TGF-beta action. Similarly, in vitro transformation resulted in only relatively small changes in the cellular binding of TGF-beta, and for those cell types that exhibited ligand-induced down-regulation of the receptor, down- regulation was not extensive. Thus the strong conservation of binding observed between cell types is also seen within a given cell type under a variety of conditions, and receptor expression appears to be essentially constitutive. Finally, the biologically inactive form of TGF-beta, which constitutes greater than 98% of autocrine TGF-beta secreted by all of the twelve different cell types assayed, was shown to be unable to bind to the receptor without prior activation in vitro. It is proposed that this may prevent premature interaction of autocrine ligand and receptor in the Golgi apparatus.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Menoret, A., Myers, L. M., Lee, S.-J., Mittler, R. S., Rossi, R. J., Vella, A. T. (2006). TGFbeta Protein Processing and Activity through TCR Triggering of Primary CD8+ T Regulatory Cells. J. Immunol. 177: 6091-6097 [Abstract] [Full Text]
Nakamura, T., Terajewicz, A., Stein-Streilein, J. (2005). Mechanisms of Peripheral Tolerance following Intracameral Inoculation Are Independent of IL-13 or STAT6. J. Immunol. 175: 2643-2646 [Abstract] [Full Text]
Mitchell, H., Choudhury, A., Pagano, R. E., Leof, E. B. (2004). Ligand-dependent and -independent Transforming Growth Factor-{beta} Receptor Recycling Regulated by Clathrin-mediated Endocytosis and Rab11. Mol. Biol. Cell 15: 4166-4178 [Abstract] [Full Text]
Hayakawa, A., Hayes, S. J., Lawe, D. C., Sudharshan, E., Tuft, R., Fogarty, K., Lambright, D., Corvera, S. (2004). Structural Basis for Endosomal Targeting by FYVE Domains. J. Biol. Chem. 279: 5958-5966 [Abstract] [Full Text]
Seto, E. S., Bellen, H. J., Lloyd, T. E. (2002). When cell biology meets development: endocytic regulation of signaling pathways. Genes Dev. 16: 1314-1336 [Full Text]
Fernandez, T., Amoroso, S., Sharpe, S., Jones, G. M., Bliskovski, V., Kovalchuk, A., Wakefield, L. M., Kim, S.-J., Potter, M., Letterio, J. J. (2002). Disruption of Transforming Growth Factor {beta} Signaling by a Novel Ligand-dependent Mechanism. JEM 195: 1247-1255 [Abstract] [Full Text]
Zieske, J. D., Hutcheon, A. E. K., Guo, X., Chung, E.-H., Joyce, N. C. (2001). TGF-{beta} Receptor Types I and II Are Differentially Expressed during Corneal Epithelial Wound Repair. IOVS 42: 1465-1471 [Abstract] [Full Text]
Doré, J. J.E. Jr., Yao, D., Edens, M., Garamszegi, N., Sholl, E. L., Leof, E. B. (2001). Mechanisms of Transforming Growth Factor-{beta} Receptor Endocytosis and Intracellular Sorting Differ between Fibroblasts and Epithelial Cells. Mol. Biol. Cell 12: 675-684 [Abstract] [Full Text]
Abe, S., Takizawa, H., Sugawara, I., Kudoh, S. (2000). Diesel Exhaust (DE)-Induced Cytokine Expression in Human Bronchial Epithelial Cells . A Study with a New Cell Exposure System to Freshly Generated DE In Vitro. Am. J. Respir. Cell Mol. Bio. 22: 296-303 [Abstract] [Full Text]
Koyama, S., Sato, E., Numanami, H., Kubo, K., Nagai, S., Izumi, T. (2000). Bradykinin Stimulates Lung Fibroblasts to Release Neutrophil and Monocyte Chemotactic Activity. Am. J. Respir. Cell Mol. Bio. 22: 75-84 [Abstract] [Full Text]
Sato, E., Koyama, S., Takamizawa, A., Masubuchi, T., Kubo, K., Robbins, R. A., Nagai, S., Izumi, T. (1999). Smoke extract stimulates lung fibroblasts to release neutrophil and monocyte chemotactic activities. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L1149-L1157 [Abstract] [Full Text]
McKaig, B. C., Makh, S. S., Hawkey, C. J., Podolsky, D. K., Mahida, Y. R. (1999). Normal human colonic subepithelial myofibroblasts enhance epithelial migration (restitution) via TGF-beta 3. Am. J. Physiol. Gastrointest. Liver Physiol. 276: G1087-G1093 [Abstract] [Full Text]
Liu, C., Yao, J., de Belle, I., Huang, R.-P., Adamson, E., Mercola, D. (1999). The Transcription Factor EGR-1 Suppresses Transformation of Human Fibrosarcoma HT1080 Cells by Coordinated Induction of Transforming Growth Factor-beta 1, Fibronectin, and Plasminogen Activator Inhibitor-1. J. Biol. Chem. 274: 4400-4411 [Abstract] [Full Text]
Han, S. H., Yea, S. S., Jeon, Y. J., Yang, K.-H., Kaminski, N. E. (1998). Transforming Growth Factor-Beta 1�(TGF-beta 1) Promotes IL-2 mRNA Expression Through the Up-regulation of NF-kappa B, AP-1 and NF-AT in EL4 Cells. J. Pharmacol. Exp. Ther. 287: 1105-1112 [Abstract] [Full Text]
Koyama, S., Sato, E., Nomura, H., Kubo, K., Miura, M., Yamashita, T., Nagai, S., Izumi, T. (1998). Bradykinin Stimulates Type II Alveolar Cells to Release Neutrophil and Monocyte Chemotactic Activity and Inflammatory Cytokines. Am. J. Pathol. 153: 1885-1893 [Abstract] [Full Text]
Masubuchi, T., Koyama, S., Sato, E., Takamizawa, A., Kubo, K., Sekiguchi, M., Nagai, S., Izumi, T. (1998). Smoke Extract Stimulates Lung Epithelial Cells to Release Neutrophil and Monocyte Chemotactic Activity. Am. J. Pathol. 153: 1903-1912 [Abstract] [Full Text]
Dore Jr., J. J.�E., Edens, M., Garamszegi, N., Leof, E. B. (1998). Heteromeric and Homomeric Transforming Growth Factor-beta Receptors Show Distinct Signaling and Endocytic Responses in Epithelial Cells. J. Biol. Chem. 273: 31770-31777 [Abstract] [Full Text]
Wu, G., Fan, R. S., Li, W., Srinivas, V., Brattain, M. G. (1998). Regulation of Transforming Growth Factor-beta Type II Receptor Expression in Human Breast Cancer MCF-7 Cells by Vitamin D3 and Its Analogues. J. Biol. Chem. 273: 7749-7756 [Abstract] [Full Text]
Smith, G. M., Hale, J. H. (1997). Macrophage/Microglia Regulation of Astrocytic Tenascin: Synergistic Action of Transforming Growth Factor-beta and Basic Fibroblast Growth Factor. J. Neurosci. 17: 9624-9633 [Abstract] [Full Text]
Anders, R. A., Arline, S. L., Doré, J. J.E., Leof, E. B. (1997). Distinct Endocytic Responses of Heteromeric and Homomeric Transforming Growth Factor beta �Receptors. Mol. Biol. Cell 8: 2133-2143 [Abstract] [Full Text]
VIGNOLA, A.�M., CHANEZ, P., CHIAPPARA, G., MERENDINO, A., PACE, E., RIZZO, A., la ROCCA, A.�M., BELLIA, V., BONSIGNORE, G., BOUSQUET, J. (1997). Transforming Growth Factor-beta Expression in Mucosal Biopsies in Asthma and Chronic Bronchitis. Am. J. Respir. Crit. Care Med. 156: 591-599 [Abstract] [Full Text]
Centrella, M., Ji, C., Casinghino, S., McCarthy, T. L. (1996). Rapid Flux in Transforming Growth Factor-beta Receptors on Bone Cells. J. Biol. Chem. 271: 18616-18622 [Abstract] [Full Text]
Boland, S, Boisvieux-Ulrich, E, Houcine, O, Baeza-Squiban, A, Pouchelet, M, Schoevaert, D, Marano, F (1996). TGF beta 1 promotes actin cytoskeleton reorganization and migratory phenotype in epithelial tracheal cells in primary culture. J. Cell Sci. 109: 2207-2219 [Abstract]
Yin, W., Smiley, E., Germiller, J., Mecham, R. P., Florer, J. B., Wenstrup, R. J., Bonadio, J. (1995). Isolation of a Novel Latent Transforming Growth Factor-[IMAGE] Binding Protein Gene (LTBP-3). J. Biol. Chem. 270: 10147-10160 [Abstract] [Full Text]
Iwasaki, S., Tsuruoka, N., Hattori, A., Sato, M., Tsujimoto, M., Kohno, M. (1995). Distribution and Characterization of Specific Cellular Binding Proteins for Bone Morphogenetic Protein-2. J. Biol. Chem. 270: 5476-5482 [Abstract] [Full Text]
Graves, D. T., Cochran, D. L. (1990). Mesenchymal Cell Growth Factors. fake Crit Rev Oral Biol Med 1: 17-36 [Full Text]
Kimchi, A, Wang, X., Weinberg, R., Cheifetz, S, Massague, J (1988). Absence of TGF-beta receptors and growth inhibitory responses in retinoblastoma cells. Science 240: 196-199 [Abstract]
Rosa, F, Roberts, A., Danielpour, D, Dart, L., Sporn, M., Dawid, I. (1988). Mesoderm induction in amphibians: the role of TGF-beta 2-like factors. Science 239: 783-785 [Abstract]
Finlay, G. A., Thannickal, V. J., Fanburg, B. L., Paulson, K. E. (2000). Transforming Growth Factor-beta 1-induced Activation of the ERK Pathway/Activator Protein-1 in Human Lung Fibroblasts Requires the Autocrine Induction of Basic Fibroblast Growth Factor. J. Biol. Chem. 275: 27650-27656 [Abstract] [Full Text]
Fanayan, S., Firth, S. M., Butt, A. J., Baxter, R. C. (2000). Growth Inhibition by Insulin-like Growth Factor-binding Protein-3 in T47D Breast Cancer Cells Requires Transforming Growth Factor-beta (TGF-beta ) and the Type II TGF-beta Receptor. J. Biol. Chem. 275: 39146-39151 [Abstract] [Full Text]
Quan, T., He, T., Voorhees, J. J., Fisher, G. J. (2001). Ultraviolet Irradiation Blocks Cellular Responses to Transforming Growth Factor-beta by Down-regulating Its Type-II Receptor and Inducing Smad7. J. Biol. Chem. 276: 26349-26356 [Abstract] [Full Text]
Parks, W. T., Frank, D. B., Huff, C., Renfrew Haft, C., Martin, J., Meng, X., de Caestecker, M. P., McNally, J. G., Reddi, A., Taylor, S. I., Roberts, A. B., Wang, T., Lechleider, R. J. (2001). Sorting Nexin 6, a Novel SNX, Interacts with the Transforming Growth Factor-beta Family of Receptor Serine-Threonine Kinases. J. Biol. Chem. 276: 19332-19339 [Abstract] [Full Text]