Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells

doi:10.1083/jcb.105.3.1215

This Article

	Full Text (PDF, 5145K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Tooze, J.
	Articles by Fuller, S. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Tooze, J.
	Articles by Fuller, S. D.


Social Bookmarking

	What's this?

ARTICLES

Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells

J Tooze, SA Tooze and SD Fuller
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Murine hepatitis virus (strain A59), (MHV-A59) is a coronavirus that buds into pre-Golgi compartments and then exploits the exocytic pathway of the host cell to reach the exterior. The fibroblastic cells in which replication of this virus is usually studied have only a constitutive exocytic pathway that the virus uses. MHV-A59 also infects, albeit inefficiently, AtT20 cells, murine pituitary tumor cells with a regulated as well as a constitutive exocytic pathway. Here we examine AtT20 cells at early times after the infection, when the Golgi apparatus retains its morphological and biochemical integrity. We observe that progeny coronavirus and secretory protein destined for the secretory granules of the regulated exocytic pathway traverse the same Golgi stacks and accumulate in the trans-Golgi network. Their pathways diverge at this site, the condensed secretory proteins including the ACTH going to the secretory granules and the coronavirus to post-Golgi transport vesicles devoid of ACTH. On very rare occasions there is missorting such that aggregates of condensed secretory proteins and viruses occur together in post-Golgi vesicles. We conclude that the constitutive and regulated exocytic pathways, identified respectively by the progeny virions and the secretory protein ACTH, diverge at the exit from the trans-Golgi network.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Schaecher, S. R., Diamond, M. S., Pekosz, A. (2008). The Transmembrane Domain of the Severe Acute Respiratory Syndrome Coronavirus ORF7b Protein Is Necessary and Sufficient for Its Retention in the Golgi Complex. J. Virol. 82: 9477-9491 [Abstract] [Full Text]
Perello, M., Stuart, R., Nillni, E. A. (2008). Prothyrotropin-releasing Hormone Targets Its Processing Products to Different Vesicles of the Secretory Pathway. J. Biol. Chem. 283: 19936-19947 [Abstract] [Full Text]
Bundgaard, J. R., Rehfeld, J. F. (2008). Distinct Linkage between Post-translational Processing and Differential Secretion of Progastrin Derivatives in Endocrine Cells. J. Biol. Chem. 283: 4014-4021 [Abstract] [Full Text]
Huang, C., Narayanan, K., Ito, N., Peters, C. J., Makino, S. (2006). Severe Acute Respiratory Syndrome Coronavirus 3a Protein Is Released in Membranous Structures from 3a Protein-Expressing Cells and Infected Cells. J. Virol. 80: 210-217 [Abstract] [Full Text]
Hogan, R. J., Gao, G., Rowe, T., Bell, P., Flieder, D., Paragas, J., Kobinger, G. P., Wivel, N. A., Crystal, R. G., Boyer, J., Feldmann, H., Voss, T. G., Wilson, J. M. (2004). Resolution of Primary Severe Acute Respiratory Syndrome-Associated Coronavirus Infection Requires Stat1. J. Virol. 78: 11416-11421 [Abstract] [Full Text]
Tan, Y.-J., Teng, E., Shen, S., Tan, T. H. P., Goh, P.-Y., Fielding, B. C., Ooi, E.-E., Tan, H.-C., Lim, S. G., Hong, W. (2004). A Novel Severe Acute Respiratory Syndrome Coronavirus Protein, U274, Is Transported to the Cell Surface and Undergoes Endocytosis. J. Virol. 78: 6723-6734 [Abstract] [Full Text]
de Haan, C. A. M., Vennema, H., Rottier, P. J. M. (2000). Assembly of the Coronavirus Envelope: Homotypic Interactions between the M Proteins. J. Virol. 74: 4967-4978 [Abstract] [Full Text]
Corse, E., Machamer, C. E. (2000). Infectious Bronchitis Virus E Protein Is Targeted to the Golgi Complex and Directs Release of Virus-Like Particles. J. Virol. 74: 4319-4326 [Abstract] [Full Text]
Bost, A. G., Carnahan, R. H., Lu, X. T., Denison, M. R. (2000). Four Proteins Processed from the Replicase Gene Polyprotein of Mouse Hepatitis Virus Colocalize in the Cell Periphery and Adjacent to Sites of Virion Assembly. J. Virol. 74: 3379-3387 [Abstract] [Full Text]
Salanueva, I. J., Carrascosa, J. L., Risco, C. (1999). Structural Maturation of the Transmissible Gastroenteritis Coronavirus. J. Virol. 73: 7952-7964 [Abstract] [Full Text]
Risco, C., Muntion, M., Enjuanes, L., Carrascosa, J. L. (1998). Two Types of Virus-Related Particles Are Found during Transmissible Gastroenteritis Virus Morphogenesis. J. Virol. 72: 4022-4031 [Abstract] [Full Text]
Klumperman, J., Kuliawat, R., Griffith, J. M., Geuze, H. J., Arvan, P. (1998). Mannose 6-Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6-positive Vesicles. JCB 141: 359-371 [Abstract] [Full Text]
Rossen, J. W.�A., de Beer, R., Godeke, G.-J., Raamsman, M. J.�B., Horzinek, M. C., Vennema, H., Rottier, P. J.�M. (1998). The Viral Spike Protein Is Not Involved in the Polarized Sorting of Coronaviruses in Epithelial Cells. J. Virol. 72: 497-503 [Abstract] [Full Text]
Fernandez, C. J., Haugwitz, M., Eaton, B., Moore, H.-P. H. (1997). Distinct Molecular Events during Secretory Granule Biogenesis Revealed by Sensitivities to Brefeldin A. Mol. Biol. Cell 8: 2171-2185 [Abstract] [Full Text]
Milgram, S., Kho, S., Martin, G., Mains, R., Eipper, B. (1997). Localization of integral membrane peptidylglycine alpha-amidating monooxygenase in neuroendocrine cells. J. Cell Sci. 110: 695-706 [Abstract]
Lin, S., Akinbi, H. T., Breslin, J. S., Weaver, T. E. (1996). Structural Requirements for Targeting of Surfactant Protein B (SP-B) to Secretory Granules in Vitro and in Vivo. J. Biol. Chem. 271: 19689-19695 [Abstract] [Full Text]
Colomer, V, Rindler, M., Lowe, A. (1994). Apical plasma membrane proteins are not obligatorily stored in secretory granules in exocrine cells. J. Cell Sci. 107: 2271-2277 [Abstract]
Braun, R E, Peschon, J J, Behringer, R R, Brinster, R L, Palmiter, R D (1989). Protamine 3'-untranslated sequences regulate temporal translational control and subcellular localization of growth hormone in spermatids of transgenic mice.. Genes Dev. 3: 793-802 [Abstract]