Synapsin I: an actin-bundling protein under phosphorylation control

doi:10.1083/jcb.105.3.1355

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Synapsin I: an actin-bundling protein under phosphorylation control

TC Petrucci and JS Morrow
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510.

Synapsin I is a neuronal phosphoprotein comprised of two closely related polypeptides with apparent molecular weights of 78,000 and 76,000. It is found in association with the small vesicles clustered at the presynaptic junction. Its precise role is unknown, although it probably participates in vesicle clustering and/or release. Synapsin I is known to associate with vesicle membranes, microtubules, and neurofilaments. We have examined the interaction of purified phosphorylated and unphosphorylated bovine and human synapsin I with tubulin and actin filaments, using cosedimentation, viscometric, electrophoretic, and morphologic assays. As purified from brain homogenates, synapsin I decreases the steady-state viscosity of solutions containing F-actin, enhances the sedimentation of actin, and bundles actin filaments. Phosphorylation by cAMP-dependent kinase has minimal effect on this interaction, while phosphorylation by brain extracts or by purified calcium- and calmodulin-dependent kinase II reduces its actin-bundling and -binding activity. Synapsin's microtubule-binding activity, conversely, is stimulated after phosphorylation by the brain extract. Two complementary peptide fragments of synapsin generated by 2-nitro-5-thiocyanobenzoic cleavage and which map to opposite ends of the molecule participate in the bundling process, either by binding directly to actin or by binding to other synapsin I molecules. 2-Nitro-5-thiocyanobenzoic peptides arising from the central portion of the molecule demonstrate neither activity. In vivo, synapsin I may link small synaptic vesicles to the actin-based cortical cytoskeleton, and coordinate their availability for release in a Ca++-dependent fashion.
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Hosaka, M., Sudhof, T. C. (1998). Synapsin III, a Novel Synapsin with an Unusual Regulation by Ca2+. J. Biol. Chem. 273: 13371-13374 [Abstract] [Full Text]
Ferreira, A, Kosik, K., Greengard, P, Han, H. (1994). Aberrant neurites and synaptic vesicle protein deficiency in synapsin II-depleted neurons. Science 264: 977-979 [Abstract]
Greengard, P, Valtorta, F, Czernik, A., Benfenati, F (1993). Synaptic vesicle phosphoproteins and regulation of synaptic function. Science 259: 780-785 [Abstract]
Malchiodi-Albedi, F, Ceccarini, M, Winkelmann, J., Morrow, J., Petrucci, T. (1993). The 270 kDa splice variant of erythrocyte beta-spectrin (beta I sigma 2) segregates in vivo and in vitro to specific domains of cerebellar neurons. J. Cell Sci. 106: 67-78 [Abstract]
Sudhof, T., Czernik, A., Kao, H., Takei, K, Johnston, P., Horiuchi, A, Kanazir, S., Wagner, M., Perin, M., De Camilli, P, al., e. (1989). Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins. Science 245: 1474-1480 [Abstract]