The subnuclear localization of tRNA ligase in yeast

doi:10.1083/jcb.105.4.1515

This Article

	Full Text (PDF, 3683K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Clark, M. W.
	Articles by Abelson, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Clark, M. W.
	Articles by Abelson, J.


Social Bookmarking

	What's this?

ARTICLES

The subnuclear localization of tRNA ligase in yeast

MW Clark and J Abelson
Division of Biology, California Institute of Technology, Pasadena 91125.

Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location to be primarily at the inner membrane of the nuclear envelope, most likely at the nuclear pore. There is a more diffuse, secondary location of ligase in a region of the nucleoplasm within 300 nm of the nuclear envelope. When the amount of ligase in the cell is increased, nuclear staining increases but staining of the nuclear envelope remains constant. This experiment indicates that there are a limited number of ligase sites at the nuclear envelope. Since the other tRNA splicing component, the endonuclease, has the characteristics of an integral membrane protein, we hypothesize that it constitutes the site for the interaction of ligase with the nuclear envelope.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Yoshihisa, T., Ohshima, C., Yunoki-Esaki, K., Endo, T. (2007). Cytoplasmic splicing of tRNA in Saccharomyces cerevisiae. GENES CELLS 12: 285-297 [Abstract] [Full Text]
Goffin, L., Vodala, S., Fraser, C., Ryan, J., Timms, M., Meusburger, S., Catimel, B., Nice, E. C., Silver, P. A., Xiao, C.-Y., Jans, D. A., Gething, M.-J. H. (2006). The Unfolded Protein Response Transducer Ire1p Contains a Nuclear Localization Sequence Recognized by Multiple beta Importins. Mol. Biol. Cell 17: 5309-5323 [Abstract] [Full Text]
Back, S. H., Lee, K., Vink, E., Kaufman, R. J. (2006). Cytoplasmic IRE1{alpha}-mediated XBP1 mRNA Splicing in the Absence of Nuclear Processing and Endoplasmic Reticulum Stress. J. Biol. Chem. 281: 18691-18706 [Abstract] [Full Text]
Shaheen, H. H., Hopper, A. K. (2005). From the Cover: Retrograde movement of tRNAs from the cytoplasm to the nucleus in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 102: 11290-11295 [Abstract] [Full Text]
Yoshihisa, T., Yunoki-Esaki, K., Ohshima, C., Tanaka, N., Endo, T. (2003). Possibility of Cytoplasmic pre-tRNA Splicing: the Yeast tRNA Splicing Endonuclease Mainly Localizes on the Mitochondria. Mol. Biol. Cell 14: 3266-3279 [Abstract] [Full Text]
Hopper, A. K., Phizicky, E. M. (2003). tRNA transfers to the limelight. Genes Dev. 17: 162-180 [Full Text]
Kendall, A., Hull, M. W., Bertrand, E., Good, P. D., Singer, R. H., Engelke, D. R. (2000). A CBF5 mutation that disrupts nucleolar localization of early tRNA biosynthesis in yeast also suppresses tRNA gene-mediated transcriptional silencing. Proc. Natl. Acad. Sci. USA 10.1073/pnas.240454997v1 [Abstract] [Full Text]
Welihinda, A. A., Tirasophon, W., Kaufman, R. J. (2000). The Transcriptional Co-activator ADA5 Is Required for HAC1 mRNA Processing in Vivo. J. Biol. Chem. 275: 3377-3381 [Abstract] [Full Text]
Urano, F, Bertolotti, A, Ron, D (2000). IRE1 and efferent signaling from the endoplasmic reticulum. J. Cell Sci. 113: 3697-3702 [Abstract]
Haze, K., Yoshida, H., Yanagi, H., Yura, T., Mori, K. (1999). Mammalian Transcription Factor ATF6 Is Synthesized as a Transmembrane Protein and Activated by Proteolysis in Response to Endoplasmic Reticulum Stress. Mol. Biol. Cell 10: 3787-3799 [Abstract] [Full Text]
Kaufman, R. J. (1999). Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev. 13: 1211-1233 [Full Text]
Hurt, E., Hannus, S., Schmelzl, B., Lau, D., Tollervey, D., Simos, G. (1999). A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants. JCB 144: 389-401 [Abstract] [Full Text]
Wolin, S. L., Matera, A. G. (1999). The trials and travels of tRNA. Genes Dev. 13: 1-10 [Full Text]
Tolerico, L. H., Benko, A. L., Aris, J. P., Stanford, D. R., Martin, N. C., Hopper, A. K. (1999). Saccharomyces cerevisiae Mod5p-II Contains Sequences Antagonistic for Nuclear and Cytosolic Locations. Genetics 151: 57-75 [Abstract] [Full Text]
Anderson, J., Phan, L., Cuesta, R., Carlson, B. A., Pak, M., Asano, K., Björk, G. R., Tamame, M., Hinnebusch, A. G. (1998). The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Genes Dev. 12: 3650-3662 [Abstract] [Full Text]
Sarkar, S., Hopper, A. K. (1998). tRNA Nuclear Export in Saccharomyces cerevisiae: In Situ Hybridization Analysis. Mol. Biol. Cell 9: 3041-3055 [Abstract] [Full Text]
Bertrand, E., Houser-Scott, F., Kendall, A., Singer, R. H., Engelke, D. R. (1998). Nucleolar localization of early tRNA processing. Genes Dev. 12: 2463-2468 [Abstract] [Full Text]
Kawahara, T., Yanagi, H., Yura, T., Mori, K. (1997). Endoplasmic Reticulum Stress-induced mRNA Splicing Permits Synthesis of Transcription Factor Hac1p/Ern4p That Activates the Unfolded Protein Response. Mol. Biol. Cell 8: 1845-1862 [Abstract] [Full Text]
Culver, G. M., McCraith, S. M., Consaul, S. A., Stanford, D. R., Phizicky, E. M. (1997). A 2'-Phosphotransferase Implicated in tRNA Splicing Is Essential in Saccharomyces cerevisiae. J. Biol. Chem. 272: 13203-13210 [Abstract] [Full Text]
Cheng, Y, Dahlberg, J., Lund, E (1995). Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport. Science 267: 1807-1810 [Abstract]
Lee, A, Tam, R, Belhumeur, P, DiPaolo, T, Clark, M. (1993). Prp20, the Saccharomyces cerevisiae homolog of the regulator of chromosome condensation, RCC1, interacts with double-stranded DNA through a multi-component complex containing GTP-binding proteins. J. Cell Sci. 106: 287-298 [Abstract]
Berman, S., Bursztajn, S, Bowen, B, Gilbert, W (1990). Localization of an acetylcholine receptor intron to the nuclear membrane. Science 247: 212-214 [Abstract]
Nakai, Y., Nakai, M., Hayashi, H., Kagamiyama, H. (2001). Nuclear Localization of Yeast Nfs1p Is Required for Cell Survival. J. Biol. Chem. 276: 8314-8320 [Abstract] [Full Text]
Kendall, A., Hull, M. W., Bertrand, E., Good, P. D., Singer, R. H., Engelke, D. R. (2000). A CBF5 mutation that disrupts nucleolar localization of early tRNA biosynthesis in yeast also suppresses tRNA gene-mediated transcriptional silencing. Proc. Natl. Acad. Sci. USA 97: 13108-13113 [Abstract] [Full Text]