A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end

doi:10.1083/jcb.105.5.2203

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A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end

DL Gard and MW Kirschner
Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco 94143.

We have isolated a protein factor from Xenopus eggs that promotes microtubule assembly in vitro. Assembly promotion was associated with a 215-kD protein after a 1,000-3,000-fold enrichment of activity. The 215- kD protein, termed Xenopus microtubule assembly protein (XMAP), binds to microtubules with a stoichiometry of 0.06 mol/mol tubulin dimer. XMAP is immunologically distinct from the Xenopus homologues to mammalian brain microtubule-associated proteins; however, protein species immunologically related to XMAP with different molecular masses are found in Xenopus neuronal tissues and testis. XMAP is unusual in that it specifically promotes microtubule assembly at the plus-end. At a molar ratio of 0.01 mol XMAP/mol tubulin the assembly rate of the microtubule plus-end is accelerated 8-fold while the assembly rate of the minus-end is increased only 1.8-fold. Under these conditions XMAP promotes a 10-fold increase in the on-rate constant (from 1.4 s- 1.microM-1 for microtubules assembled from pure tubulin to 15 s- 1.microM-1), and a 10-fold decrease in off-rate constant (from 340 to 34 s-1). Given its stoichiometry in vivo, XMAP must be the major microtubule assembly factor in the Xenopus egg. XMAP is phosphorylated during M-phase of both meiotic and mitotic cycles, suggesting that its activity may be regulated during the cell cycle.
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Kawamura, E., Wasteneys, G. O. (2008). MOR1, the Arabidopsis thaliana homologue of Xenopus MAP215, promotes rapid growth and shrinkage, and suppresses the pausing of microtubules in vivo. J. Cell Sci. 121: 4114-4123 [Abstract] [Full Text]
Barr, A. R., Gergely, F. (2008). MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics. Mol. Cell. Biol. 28: 7199-7211 [Abstract] [Full Text]
Albee, A. J., Wiese, C. (2008). Xenopus TACC3/Maskin Is Not Required for Microtubule Stability but Is Required for Anchoring Microtubules at the Centrosome. Mol. Biol. Cell 19: 3347-3356 [Abstract] [Full Text]
Bellanger, J.-M., Carter, J. C., Phillips, J. B., Canard, C., Bowerman, B., Gonczy, P. (2007). ZYG-9, TAC-1 and ZYG-8 together ensure correct microtubule function throughout the cell cycle of C. elegans embryos. J. Cell Sci. 120: 2963-2973 [Abstract] [Full Text]
Enke, C., Zekert, N., Veith, D., Schaaf, C., Konzack, S., Fischer, R. (2007). Aspergillus nidulans Dis1/XMAP215 Protein AlpA Localizes to Spindle Pole Bodies and Microtubule Plus Ends and Contributes to Growth Directionality. Eukaryot Cell 6: 555-562 [Abstract] [Full Text]
Bonfils, C., Bec, N., Lacroix, B., Harricane, M.-C., Larroque, C. (2007). Kinetic Analysis of Tubulin Assembly in the Presence of the Microtubule-associated Protein TOGp. J. Biol. Chem. 282: 5570-5581 [Abstract] [Full Text]
Wiese, C., Zheng, Y. (2006). Microtubule nucleation: {gamma}-tubulin and beyond. J. Cell Sci. 119: 4143-4153 [Abstract] [Full Text]
Efimov, V. P., Zhang, J., Xiang, X. (2006). CLIP-170 Homologue and NUDE Play Overlapping Roles in NUDF Localization in Aspergillus nidulans. Mol. Biol. Cell 17: 2021-2034 [Abstract] [Full Text]
Al-Bassam, J., van Breugel, M., Harrison, S. C., Hyman, A. (2006). Stu2p binds tubulin and undergoes an open-to-closed conformational change.. JCB 172: 1009-1022 [Abstract] [Full Text]
Kawamura, E., Himmelspach, R., Rashbrooke, M. C., Whittington, A. T., Gale, K. R., Collings, D. A., Wasteneys, G. O. (2006). MICROTUBULE ORGANIZATION 1 Regulates Structure and Function of Microtubule Arrays during Mitosis and Cytokinesis in the Arabidopsis Root. Plant Physiol. 140: 102-114 [Abstract] [Full Text]
VanBuren, V., Cassimeris, L., Odde, D. J. (2005). Mechanochemical Model of Microtubule Structure and Self-Assembly Kinetics. Biophys. J 89: 2911-2926 [Abstract] [Full Text]
Peset, I., Seiler, J., Sardon, T., Bejarano, L. A., Rybina, S., Vernos, I. (2005). Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosis. JCB 170: 1057-1066 [Abstract] [Full Text]
O'Brien, L. L., Albee, A. J., Liu, L., Tao, W., Dobrzyn, P., Lizarraga, S. B., Wiese, C. (2005). The Xenopus TACC Homologue, Maskin, Functions in Mitotic Spindle Assembly. Mol. Biol. Cell 16: 2836-2847 [Abstract] [Full Text]
Hamada, T., Igarashi, H., Itoh, T. J., Shimmen, T., Sonobe, S. (2004). Characterization of a 200 kDa Microtubule-associated Protein of Tobacco BY-2 Cells, a Member of the XMAP215/MOR1 Family. Plant Cell Physiol 45: 1233-1242 [Abstract] [Full Text]
Cassimeris, L., Morabito, J. (2004). TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly. Mol. Biol. Cell 15: 1580-1590 [Abstract] [Full Text]
Sato, M., Vardy, L., Angel Garcia, M., Koonrugsa, N., Toda, T. (2004). Interdependency of Fission Yeast Alp14/TOG and Coiled Coil Protein Alp7 in Microtubule Localization and Bipolar Spindle Formation. Mol. Biol. Cell 15: 1609-1622 [Abstract] [Full Text]
Cao, T. T., Chang, W., Masters, S. E., Mooseker, M. S. (2004). Myosin-Va Binds to and Mechanochemically Couples Microtubules to Actin Filaments. Mol. Biol. Cell 15: 151-161 [Abstract] [Full Text]
Pearson, C. G., Maddox, P. S., Zarzar, T. R., Salmon, E.D., Bloom, K. (2003). Yeast Kinetochores Do Not Stabilize Stu2p-dependent Spindle Microtubule Dynamics. Mol. Biol. Cell 14: 4181-4195 [Abstract] [Full Text]
Shirasu-Hiza, M., Coughlin, P., Mitchison, T. (2003). Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification. JCB 161: 349-358 [Abstract] [Full Text]
van Breugel, M., Drechsel, D., Hyman, A. (2003). Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer. JCB 161: 359-369 [Abstract] [Full Text]
Jin, Q.-W., Pidoux, A. L., Decker, C., Allshire, R. C., Fleig, U. (2002). The Mal2p Protein Is an Essential Component of the Fission Yeast Centromere. Mol. Cell. Biol. 22: 7168-7183 [Abstract] [Full Text]
Rehberg, M., Graf, R. (2002). Dictyostelium EB1 Is a Genuine Centrosomal Component Required for Proper Spindle Formation. Mol. Biol. Cell 13: 2301-2310 [Abstract] [Full Text]
Yasuhara, H., Muraoka, M., Shogaki, H., Mori, H., Sonobe, S. (2002). TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue. Plant Cell Physiol 43: 595-603 [Abstract] [Full Text]
Cassimeris, L., Gard, D., Tran, P. T., Erickson, H. P. (2002). XMAP215 is a long thin molecule that does not increase microtubule stiffness. J. Cell Sci. 114: 3025-3033 [Abstract] [Full Text]
Kinoshita, K., Arnal, I., Desai, A., Drechsel, D. N., Hyman, A. A. (2001). Reconstitution of Physiological Microtubule Dynamics Using Purified Components. Science 294: 1340-1343 [Abstract] [Full Text]
Severin, F., Habermann, B., Huffaker, T., Hyman, T. (2001). Stu2 Promotes Mitotic Spindle Elongation in Anaphase. JCB 153: 435-442 [Abstract] [Full Text]
Ohkura, H., Garcia, M. A., Toda, T. (2001). Dis1/TOG universal microtubule adaptors - one MAP for all?. J. Cell Sci. 114: 3805-3812 [Abstract] [Full Text]
Dionne, M. A., Sanchez, A., Compton, D. A. (2000). ch-TOGp Is Required for Microtubule Aster Formation in a Mammalian Mitotic Extract. J. Biol. Chem. 275: 12346-12352 [Abstract] [Full Text]
Inoue, Y. H., do Carmo Avides, M., Shiraki, M., Deak, P., Yamaguchi, M., Nishimoto, Y., Matsukage, A., Glover, D. M. (2000). Orbit, a Novel Microtubule-associated Protein Essential for Mitosis in Drosophila melanogaster. JCB 149: 153-166 [Abstract] [Full Text]
Graf, R, Daunderer, C, Schliwa, M (2000). Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication. J. Cell Sci. 113: 1747-1758 [Abstract]
Cullen, C. F., Deak, P., Glover, D. M., Ohkura, H. (1999). mini spindles: A Gene Encoding a Conserved Microtubule-associated Protein Required for the Integrity of the Mitotic Spindle in Drosophila. JCB 146: 1005-1018 [Abstract] [Full Text]
Scheel, J., Pierre, P., Rickard, J. E., Diamantopoulos, G. S., Valetti, C., van der Goot, F. G., Haner, M., Aebi, U., Kreis, T. E. (1999). Purification and Analysis of Authentic CLIP-170 and Recombinant Fragments. J. Biol. Chem. 274: 25883-25891 [Abstract] [Full Text]
Tirnauer, J. S., O'Toole, E., Berrueta, L., Bierer, B. E., Pellman, D. (1999). Yeast Bim1p Promotes the G1-specific Dynamics of Microtubules. JCB 145: 993-1007 [Abstract] [Full Text]
Wilde, A., Zheng, Y. (1999). Stimulation of Microtubule Aster Formation and Spindle Assembly by the Small GTPase Ran. Science 284: 1359-1362 [Abstract] [Full Text]
Gonczy, P., Schnabel, H., Kaletta, T., Amores, A. D., Hyman, T., Schnabel, R. (1999). Dissection of Cell Division Processes in the One Cell Stage Caenorhabditis elegans Embryo by Mutational Analysis. JCB 144: 927-946 [Abstract] [Full Text]
Cha, B, Cassimeris, L, Gard, D. (1999). XMAP230 is required for normal spindle assembly in vivo and in vitro. J. Cell Sci. 112: 4337-4346 [Abstract]
Diamantopoulos, G. S., Perez, F., Goodson, H. V., Batelier, G., Melki, R., Kreis, T. E., Rickard, J. E. (1999). Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly. JCB 144: 99-112 [Abstract] [Full Text]
Matthews, L. R., Carter, P., Thierry-Mieg, D., Kemphues, K. (1998). ZYG-9, A Caenorhabditis elegans Protein Required for Microtubule Organization and Function, Is a Component of Meiotic and Mitotic Spindle Poles. JCB 141: 1159-1168 [Abstract] [Full Text]
Cha, B., Error, B, Gard, D. (1998). XMAP230 is required for the assembly and organization of acetylated microtubules and spindles in Xenopus oocytes and eggs. J. Cell Sci. 111: 2315-2327 [Abstract]
Charrasse, S, Schroeder, M, Gauthier-Rouviere, C, Ango, F, Cassimeris, L, Gard, D., Larroque, C (1998). The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215. J. Cell Sci. 111: 1371-1383 [Abstract]
Wang, P. J., Huffaker, T. C. (1997). Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body. JCB 139: 1271-1280 [Abstract] [Full Text]
Andersen, S. S.L., Karsenti, E. (1997). XMAP310: A Xenopus Rescue-promoting Factor Localized to the Mitotic Spindle. JCB 139: 975-983 [Abstract] [Full Text]
Tran, P.T., Walker, R.A., Salmon, E.D. (1997). A Metastable Intermediate State of Microtubule Dynamic Instability That Differs Significantly between Plus and Minus Ends. JCB 138: 105-117 [Abstract] [Full Text]
Kidd, D, Raff, J. (1997). LK6, a short lived protein kinase in Drosophila that can associate with microtubules and centrosomes. J. Cell Sci. 110: 209-219 [Abstract]
Brisch, E, Daggett, M., Suprenant, K. (1996). Cell cycle-dependent phosphorylation of the 77 kDa echinoderm microtubule-associated protein (EMAP) in vivo and association with the p34cdc2 kinase. J. Cell Sci. 109: 2885-2893 [Abstract]
Ohta, K, Shiina, N, Okumura, E, Hisanaga, S, Kishimoto, T, Endo, S, Gotoh, Y, Nishida, E, Sakai, H (1993). Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: involvement of phosphorylation and accumulation of pericentriolar material. J. Cell Sci. 104: 125-137 [Abstract]
Spittle, C., Charrasse, S., Larroque, C., Cassimeris, L. (2000). The Interaction of TOGp with Microtubules and Tubulin. J. Biol. Chem. 275: 20748-20753 [Abstract] [Full Text]
Lin, H., de Carvalho, P., Kho, D., Tai, C.-Y., Pierre, P., Fink, G. R., Pellman, D. (2001). Polyploids require Bik1 for kinetochore-microtubule attachment. JCB 155: 1173-1184 [Abstract] [Full Text]