Mobility of filamentous actin in living cytoplasm

doi:10.1083/jcb.105.6.2811

This Article

	Full Text (PDF, 1751K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wang, Y. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wang, Y. L.


Social Bookmarking

	What's this?

ARTICLES

Mobility of filamentous actin in living cytoplasm

YL Wang
Doris W. Neustadt Laboratory of Cellular Structure, Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

Filamentous actin in living cultured cells was labeled by microinjecting trace amounts of rhodamine-phalloidin (rh-pha) as a specific, high-affinity probe. The microinjection caused no detectable effect on cell morphology or cell division. The distribution of rh-pha- labeled filaments was then examined in dividing cells using image- intensified fluorescence microscopy, and the exchangeability of labeled filaments along stress fibers was studied during interphase using fluorescence recovery after photobleaching. rh-pha showed a rapid concentration at the contractile ring during cell division. In addition, recovery of fluorescence after photobleaching occurred along stress fibers with a halftime as short as 8 min. These observations suggest that at least some actin filaments undergo continuous movement and reorganization in living cells. This dynamic process may play an important role in various cellular functions.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Le Clainche, C., Carlier, M.-F. (2008). Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration. Physiol. Rev. 88: 489-513 [Abstract] [Full Text]
McDonald, D., Carrero, G., Andrin, C., de Vries, G., Hendzel, M. J. (2006). Nucleoplasmic {beta}-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations.. JCB 172: 541-552 [Abstract] [Full Text]
Stricker, J., Maddox, P., Salmon, E. D., Erickson, H. P. (2002). Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc. Natl. Acad. Sci. USA 10.1073/pnas.052595099v1 [Abstract] [Full Text]
Henson, J. H., Svitkina, T. M., Burns, A. R., Hughes, H. E., MacPartland, K. J., Nazarian, R., Borisy, G. G. (1999). Two Components of Actin-based Retrograde Flow in Sea Urchin Coelomocytes. Mol. Biol. Cell 10: 4075-4090 [Abstract] [Full Text]
Almenar-Queralt, A, Gregorio, C., Fowler, V. (1999). Tropomodulin assembles early in myofibrillogenesis in chick skeletal muscle: evidence that thin filaments rearrange to form striated myofibrils. J. Cell Sci. 112: 1111-1123 [Abstract]
Fukui, Y, Kitanishi-Yumura, T, Yumura, S (1999). Myosin II-independent F-actin flow contributes to cell locomotion in dictyostelium. J. Cell Sci. 112: 877-886 [Abstract]
Becker, K., Hart, N. (1999). Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis. J. Cell Sci. 112: 97-110 [Abstract]
Schafer, D. A., Welch, M. D., Machesky, L. M., Bridgman, P. C., Meyer, S. M., Cooper, J. A. (1998). Visualization and Molecular Analysis of Actin Assembly in Living Cells. JCB 143: 1919-1930 [Abstract] [Full Text]
Cipolla, M. J., Osol, G., Kontos, H. A. (1998). Vascular Smooth Muscle Actin Cytoskeleton in Cerebral Artery Forced Dilatation � Editorial Comment. Stroke 29: 1223-1228 [Abstract] [Full Text]
Yumura, S, Fukui, Y (1998). Spatiotemporal dynamics of actin concentration during cytokinesis and locomotion in Dictyostelium. J. Cell Sci. 111: 2097-2108 [Abstract]
Verkhovsky, A., Svitkina, T., Borisy, G. (1997). Polarity sorting of actin filaments in cytochalasin-treated fibroblasts. J. Cell Sci. 110: 1693-1704 [Abstract]
Hird, S (1996). Cortical actin movements during the first cell cycle of the Caenorhabditis elegans embryo. J. Cell Sci. 109: 525-533 [Abstract]
Stricker, J., Maddox, P., Salmon, E. D., Erickson, H. P. (2002). Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc. Natl. Acad. Sci. USA 99: 3171-3175 [Abstract] [Full Text]