Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level

doi:10.1083/jcb.106.1.141

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Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level

S Khawaja, GG Gundersen and JC Bulinski
Department of Biology, University of California, Los Angeles 90024.

Interphase cultured monkey kidney (TC-7) cells contain distinct subsets of cellular microtubules (MTs) enriched in posttranslationally detyrosinated (Glu) or tyrosinated (Tyr) alpha tubulin (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779-789). To determine the relative stability of these subsets of MTs, we subjected TC-7 cells to treatments that slowly depolymerized MTs. We found Glu MTs to be more resistant than Tyr MTs to depolymerization by nocodazole in living cells, and to depolymerization by dilution in detergent- permeabilized cell models. However, in cold-treated cells, Glu and Tyr MTs did not differ significantly in their stability. Digestion of permeabilized cell models with pancreatic carboxypeptidase A, to generate Glu MTs from endogenous Tyr MTs, did not significantly alter the resistance of the endogenous Tyr MTs toward dilution-induced depolymerization. Furthermore, in human fibroblasts that contained no distinct Glu MTs, we observed a population of nocodazole-resistant MTs. These data suggest that Glu MTs possess enhanced stability against end- mediated depolymerization, yet detyrosination alone appears to be insufficient to confer this enhanced stability.
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Leung, C. L., Sun, D., Zheng, M., Knowles, D. R., Liem, R. K.H. (1999). Microtubule Actin Cross-linking Factor (MACF): A Hybrid of Dystonin and Dystrophin that Can Interact with the Actin and Microtubule Cytoskeletons. JCB 147: 1275-1286 [Abstract] [Full Text]
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Goold, R., Owen, R, Gordon-Weeks, P. (1999). Glycogen synthase kinase 3beta phosphorylation of microtubule-associated protein 1B regulates the stability of microtubules in growth cones. J. Cell Sci. 112: 3373-3384 [Abstract]
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Merrick, S. E., Trojanowski, J. Q., Lee, V. M.-Y. (1997). Selective Destruction of Stable Microtubules and Axons by Inhibitors of Protein Serine/Threonine Phosphatases in Cultured Human Neurons (NT2N Cells). J. Neurosci. 17: 5726-5737 [Abstract] [Full Text]
Minin, A. (1997). Dispersal of Golgi apparatus in nocodazole-treated fibroblasts is a kinesin-driven process. J. Cell Sci. 110: 2495-2505 [Abstract]
Nguyen, H., Chari, S, Gruber, D, Lue, C., Chapin, S., Bulinski, J. (1997). Overexpression of full- or partial-length MAP4 stabilizes microtubules and alters cell growth. J. Cell Sci. 110: 281-294 [Abstract]
Williamson, T., Gordon-Weeks, P.�R., Schachner, M., Taylor, J. (1996). Microtubule reorganization is obligatory for growth cone�turning. Proc. Natl. Acad. Sci. USA 93: 15221-15226 [Abstract] [Full Text]
Laurent, M, Fleury, A (1996). Hysteretic behavior and differential apparent stability properties of microtubule species emerge from the regulation of post-translational modifications of microtubules. J. Cell Sci. 109: 419-428 [Abstract]
Liao, G, Nagasaki, T, Gundersen, G. (1995). Low concentrations of nocodazole interfere with fibroblast locomotion without significantly affecting microtubule level: implications for the role of dynamic microtubules in cell locomotion. J. Cell Sci. 108: 3473-3483 [Abstract]
Robinson, J., Vandre, D. (1995). Stimulus-dependent alterations in macrophage microtubules: increased tubulin polymerization and detyrosination. J. Cell Sci. 108: 645-655 [Abstract]
Paturle-Lafanechere, L, Manier, M, Trigault, N, Pirollet, F, Mazarguil, H, Job, D (1994). Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies. J. Cell Sci. 107: 1529-1543 [Abstract]
Gundersen, G., Kim, I, Chapin, C. (1994). Induction of stable microtubules in 3T3 fibroblasts by TGF-beta and serum. J. Cell Sci. 107: 645-659 [Abstract]
Baas, P., Pienkowski, T., Cimbalnik, K., Toyama, K, Bakalis, S, Ahmad, F., Kosik, K. (1994). Tau confers drug stability but not cold stability to microtubules in living cells. J. Cell Sci. 107: 135-143 [Abstract]
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