The Journal of Cell Biology, Vol 106, 213-223, Copyright © 1988 by The Rockefeller University Press

ARTICLES

The L2/HNK-1 carbohydrate of neural cell adhesion molecules is involved in cell interactions

V Kunemund, FB Jungalwala, G Fischer, DK Chou, G Keilhauer and M Schachner
Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.

We investigated whether the L2/HNK-1 carbohydrate epitope, expressed by two unusual glycolipids and several neural adhesion molecules, including L1, neural cell adhesion molecule, J1, and the myelin- associated glycoprotein, is involved in adhesion. Monoclonal L2 antibodies, the L2/HNK-1-reactive, sulfate-3-glucuronyl residue carrying glycolipids (L2 glycolipid) and a tetrasaccharide derived from the L2 glycolipid (L2 tetrasaccharide) were added to microexplant cultures of early postnatal mouse cerebellum, and cell migration and process extension were monitored. On the substrate poly-D-lysine, Fab fragments of L2 antibodies, L2 glycolipid, and L2 tetrasaccharide inhibited outgrowth of astrocytic processes and migration of cell bodies, but only L2 glycolipid and L2 tetrasaccharide reduced neurite outgrowth. On laminin, L2 antibodies, L2 glycolipid, and L2 tetrasaccharide inhibited outgrowth of astrocytic processes. Additionally, L2 glycolipid and L2 tetrasaccharide inhibited cell migration and neurite outgrowth. Several negatively charged glycolipids, lipids, and saccharides were tested for control and found to have no effect on outgrowth patterns, except for sulfatide and heparin, which modified outgrowth patterns in a similar fashion as L2 glycolipid and L2 tetrasaccharide. On astrocytes none of the tested compounds interfered with explant outgrowth. In short-term adhesion assays L2 glycolipid, sulfatide, and heparin inhibited adhesion of neural cells to laminin. L2 glycolipid and sulfatide interfered with neuron to astrocyte and astrocyte to astrocyte adhesion, but not with neuron-neuron adhesion. The most straightforward interpretation of these observations is that the L2/HNK-1 carbohydrate and the sulfated carbohydrates, sulfatide and heparin, act as ligands in cell adhesion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Fongmoon, D., Shetty, A. K., Basappa, , Yamada, S., Sugiura, M., Kongtawelert, P., Sugahara, K. (2007). Chondroitinase-mediated Degradation of Rare 3-O-Sulfated Glucuronic Acid in Functional Oversulfated Chondroitin Sulfate K and E. J. Biol. Chem. 282: 36895-36904 [Abstract] [Full Text]  
• Kakuda, S., Sato, Y., Tonoyama, Y., Oka, S., Kawasaki, T. (2005). Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate. Glycobiology 15: 203-210 [Abstract] [Full Text]  
• Fan, J., Tam, P., Woude, G. V., Ren, Y. (2004). Normalization and analysis of cDNA microarrays using within-array replications applied to neuroblastoma cell response to a cytokine. Proc. Natl. Acad. Sci. USA 101: 1135-1140 [Abstract] [Full Text]  
• Uusitalo, M., Schlotzer-Schrehardt, U., Kivela, T. (2003). Ultrastructural Localization of the HNK-1 Carbohydrate Epitope to Glial and Neuronal Cells of the Human Retina. IOVS 44: 961-964 [Abstract] [Full Text]  
• Yamamoto, S., Oka, S., Inoue, M., Shimuta, M., Manabe, T., Takahashi, H., Miyamoto, M., Asano, M., Sakagami, J., Sudo, K., Iwakura, Y., Ono, K., Kawasaki, T. (2002). Mice Deficient in Nervous System-specific Carbohydrate Epitope HNK-1 Exhibit Impaired Synaptic Plasticity and Spatial Learning. J. Biol. Chem. 277: 27227-27231 [Abstract] [Full Text]  
• Ong, E., Suzuki, M., Belot, F., Yeh, J.-C., Franceschini, I., Angata, K., Hindsgaul, O., Fukuda, M. (2002). Biosynthesis of HNK-1 Glycans on O-Linked Oligosaccharides Attached to the Neural Cell Adhesion Molecule (NCAM). THE REQUIREMENT FOR CORE 2 beta 1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE AND THE MUSCLE-SPECIFIC DOMAIN IN NCAM. J. Biol. Chem. 277: 18182-18190 [Abstract] [Full Text]  
• Liedtke, S., Geyer, H., Wuhrer, M., Geyer, R., Frank, G., Gerardy-Schahn, R., Zahringer, U., Schachner, M. (2001). Characterization of N-glycans from mouse brain neural cell adhesion molecule. Glycobiology 11: 373-384 [Abstract] [Full Text]  
• Ong, E., Yeh, J.-C., Ding, Y., Hindsgaul, O., Pedersen, L. C., Negishi, M., Fukuda, M. (1999). Structure and Function of HNK-1 Sulfotransferase. IDENTIFICATION OF DONOR AND ACCEPTOR BINDING SITES BY SITE-DIRECTED MUTAGENESIS. J. Biol. Chem. 274: 25608-25612 [Abstract] [Full Text]  
• Niehaus, A., Stegmuller, J., Diers-Fenger, M., Trotter, J. (1999). Cell-Surface Glycoprotein of Oligodendrocyte Progenitors Involved in Migration. J. Neurosci. 19: 4948-4961 [Abstract] [Full Text]  
• Shimoda, Y., Tajima, Y., Nagase, T., Harii, K., Osumi, N., Sanai, Y. (1999). Cloning and Expression of a Novel Galactoside beta 1,3-Glucuronyltransferase Involved in the Biosynthesis of HNK-1 Epitope. J. Biol. Chem. 274: 17115-17122 [Abstract] [Full Text]  
• Garwood, J., Schnadelbach, O., Clement, A., Schutte, K., Bach, A., Faissner, A. (1999). DSD-1-Proteoglycan Is the Mouse Homolog of Phosphacan and Displays Opposing Effects on Neurite Outgrowth Dependent on Neuronal Lineage. J. Neurosci. 19: 3888-3899 [Abstract] [Full Text]  
• Miura, R., Aspberg, A., Ethell, I. M., Hagihara, K., Schnaar, R. L., Ruoslahti, E., Yamaguchi, Y. (1999). The Proteoglycan Lectin Domain Binds Sulfated Cell Surface Glycolipids and Promotes Cell Adhesion. J. Biol. Chem. 274: 11431-11438 [Abstract] [Full Text]  
• Anderson, R., Key, B (1999). Novel guidance cues during neuronal pathfinding in the early scaffold of axon tracts in the rostral brain. Development 126: 1859-1868 [Abstract]  
• Moorman, A. F.M., de Jong, F., Denyn, M. M.F.J., Lamers, W. H. (1998). Development of the Cardiac Conduction System. Circ. Res. 82: 629-644 [Full Text]  
• Chou, D. K. H., Tobet, S. A., Jungalwala, F. B. (1998). Restoration of Synthesis of Sulfoglucuronylglycolipids in Cerebellar Granule Neurons Promotes Dedifferentiation and Neurite Outgrowth. J. Biol. Chem. 273: 8508-8515 [Abstract] [Full Text]  
• Sato, T., Furukawa, K., Bakker, H., Van den Eijnden, D. H., Van Die, I. (1998). Molecular cloning of a human cDNA encoding beta -1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta -1,4-galactosyltransferase. Proc. Natl. Acad. Sci. USA 95: 472-477 [Abstract] [Full Text]  
• Kinoshita, A., Yamada, S., Haslam, S. M., Morris, H. R., Dell, A., Sugahara, K. (1997). Novel Tetrasaccharides Isolated from Squid Cartilage Chondroitin Sulfate E Contain Unusual Sulfated Disaccharide Units GlcA(3-O-sulfate)beta 1-3GalNAc(6-O-sulfate) or GlcA(3-O-sulfate)beta 1-3GalNAc(4,6-O-disulfate). J. Biol. Chem. 272: 19656-19665 [Abstract] [Full Text]  
• Terayama, K., Oka, S., Seiki, T., Miki, Y., Nakamura, A., Kozutsumi, Y., Takio, K., Kawasaki, T. (1997). Cloning and functional expression of a novel glucuronyltransferase involved in the biosynthesis of the carbohydrate epitope HNK-1. Proc. Natl. Acad. Sci. USA 94: 6093-6098 [Abstract] [Full Text]  
• Kudo, M., Kitajima, K., Inoue, S., Shiokawa, K., Morris, H. R., Dell, A., Inoue, Y. (1996). Characterization of the Major Core Structures of the alpha 2right-arrow 8-linked Polysialic Acid-containing Glycan Chains Present in Neural Cell Adhesion Molecule in Embryonic Chick Brains. J. Biol. Chem. 271: 32667-32677 [Abstract] [Full Text]  
• Chou, D. K.H., Jungalwala, F. B. (1996). N-Acetylglucosaminyl Transferase Regulates the Expression of the Sulfoglucuronyl Glycolipids in Specific Cell Types in Cerebellum during Development. J. Biol. Chem. 271: 28868-28874 [Abstract] [Full Text]  
• Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., Dell, A. (1996). Novel Sulfated Oligosaccharides Containing 3-O-Sulfated Glucuronic Acid from King Crab Cartilage Chondroitin Sulfate K. UNEXPECTED DEGRADATION BY CHONDROITINASE ABC. J. Biol. Chem. 271: 26745-26754 [Abstract] [Full Text]  
• Marvin, K. W., Fujimoto, W., Jetten, A. M. (1995). Identification and Characterization of a Novel Squamous Cell-associated Gene Related to PMP22. J. Biol. Chem. 270: 28910-28916 [Abstract] [Full Text]  
• Shibata, S., Takagaki, K., Nakamura, T., Izumi, J., Kojima, K., Kato, I., Endo, M. (1995). HNK-1-reactive Novel Oligosaccharide, Sulfate-O-3GlcA[IMAGE]1- 4Xyl[IMAGE]1-(4-methylumbelliferone), Synthesized by Cultured Human Skin Fibroblasts. J. Biol. Chem. 270: 13794-13798 [Abstract] [Full Text]  
• Panjwani, N., Zhao, Z., Ahmad, S., Yang, Z., Jungalwala, F., Baum, J. (1995). Neolactoglycosphingolipids, Potential Mediators of Corneal Epithelial Cell Migration. J. Biol. Chem. 270: 14015-14023 [Abstract] [Full Text]  
• Mahanthappa, N., Cooper, D., Barondes, S., Schwarting, G. (1994). Rat olfactory neurons can utilize the endogenous lectin, L-14, in a novel adhesion mechanism. Development 120: 1373-1384 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents