Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene [published erratum appears in J Cell Biol 1988 Jun;106(6):2225]

doi:10.1083/jcb.106.3.585

This Article

	Full Text (PDF, 2640K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gruenbaum, Y.
	Articles by Fisher, P. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gruenbaum, Y.
	Articles by Fisher, P. A.


Social Bookmarking

	What's this?

ARTICLES

Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene [published erratum appears in J Cell Biol 1988 Jun;106(6):2225]

Y Gruenbaum, Y Landesman, B Drees, JW Bare, H Saumweber, MR Paddy, JW Sedat, DE Smith, BM Benton and PA Fisher
Department of Biochemistry and Biophysics, University of California at San Francisco 94143.

A cDNA clone encoding a portion of Drosophila nuclear lamins Dm1 and Dm2 has been identified by screening a lambda-gt11 cDNA expression library using Drosophila lamin-specific monoclonal antibodies. Two different developmentally regulated mRNA species were identified by Northern blot analysis using the initial cDNA as a probe, and full- length cDNA clones, apparently corresponding to each message, have been isolated. In vitro transcription of both full-length cDNA clones in a pT7 transcription vector followed by in vitro translation in wheat germ lysate suggests that both clones encode lamin Dm0, the polypeptide precursor of lamins Dm1 and Dm2. Nucleotide sequence analyses confirm the impression that both cDNA clones code for the identical polypeptide, which is highly homologous with human lamins A and C as well as with mammalian intermediate filament proteins. The two clones differ in their 3'-untranslated regions. In situ hybridization of lamin cDNA clones to Drosophila polytene chromosomes shows only a single locus of hybridization at or near position 25F on the left arm of chromosome 2. Southern blot analyses of genomic DNA are consistent with the notion that a single or only a few highly similar genes encoding Drosophila nuclear lamin Dm0 exist in the genome.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Cai, W., Bao, X., Deng, H., Jin, Y., Girton, J., Johansen, J., Johansen, K. M. (2008). RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila. Development 135: 2917-2925 [Abstract] [Full Text]
Mattout, A., Goldberg, M., Tzur, Y., Margalit, A., Gruenbaum, Y. (2007). Specific and conserved sequences in D. melanogaster and C. elegans lamins and histone H2A mediate the attachment of lamins to chromosomes. J. Cell Sci. 120: 77-85 [Abstract] [Full Text]
Bao, X., Zhang, W., Krencik, R., Deng, H., Wang, Y., Girton, J., Johansen, J., Johansen, K. M. (2005). The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells. J. Cell Sci. 118: 5079-5087 [Abstract] [Full Text]
Claycomb, J. M., MacAlpine, D. M., Evans, J. G., Bell, S. P., Orr-Weaver, T. L. (2002). Visualization of replication initiation and elongation in Drosophila. JCB 159: 225-236 [Abstract] [Full Text]
Goldman, R. D., Gruenbaum, Y., Moir, R. D., Shumaker, D. K., Spann, T. P. (2002). Nuclear lamins: building blocks of nuclear architecture. Genes Dev. 16: 533-547 [Full Text]
Liu, J., Ben-Shahar, T. R., Riemer, D., Treinin, M., Spann, P., Weber, K., Fire, A., Gruenbaum, Y. (2000). Essential Roles for Caenorhabditis elegans Lamin Gene in Nuclear Organization, Cell Cycle Progression, and Spatial Organization of Nuclear Pore Complexes. Mol. Biol. Cell 11: 3937-3947 [Abstract] [Full Text]
Goldberg, M., Harel, A., Brandeis, M., Rechsteiner, T., Richmond, T. J., Weiss, A. M., Gruenbaum, Y. (1999). The tail domain of lamin Dm0 binds histones H2A and H2B. Proc. Natl. Acad. Sci. USA 96: 2852-2857 [Abstract] [Full Text]
Goldberg, M., Lu, H., Stuurman, N., Ashery-Padan, R., Weiss, A. M., Yu, J., Bhattacharyya, D., Fisher, P. A., Gruenbaum, Y., Wolfner, M. F. (1998). Interactions among Drosophila Nuclear Envelope Proteins Lamin, Otefin, and YA. Mol. Cell. Biol. 18: 4315-4323 [Abstract] [Full Text]
Rzepecki, R, Bogachev, S., Kokoza, E, Stuurman, N, Fisher, P. (1998). In vivo association of lamins with nucleic acids in Drosophila melanogaster. J. Cell Sci. 111: 121-129 [Abstract]
Lenz-Bohme, B., Wismar, J., Fuchs, S., Reifegerste, R., Buchner, E., Betz, H., Schmitt, B. (1997). Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae. JCB 137: 1001-1016 [Abstract] [Full Text]
Klapper, M, Exner, K, Kempf, A, Gehrig, C, Stuurman, N, Fisher, P., Krohne, G (1997). Assembly of A- and B-type lamins studied in vivo with the baculovirus system. J. Cell Sci. 110: 2519-2532 [Abstract]
Paddy, M. R., Saumweber, H., Agard, D. A., Sedat, J. W. (1996). Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos. J. Cell Sci. 109: 591-607 [Abstract]
Riemer, D, Stuurman, N, Berrios, M, Hunter, C, Fisher, P., Weber, K (1995). Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins. J. Cell Sci. 108: 3189-3198 [Abstract]
Stuurman, N, Maus, N, Fisher, P. (1995). Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J. Cell Sci. 108: 3137-3144 [Abstract]
Maus, N, Stuurman, N, Fisher, P. (1995). Disassembly of the Drosophila nuclear lamina in a homologous cell-free system. J. Cell Sci. 108: 2027-2035 [Abstract]
Lantz, V, Chang, J S, Horabin, J I, Bopp, D, Schedl, P (1994). The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.. Genes Dev. 8: 598-613 [Abstract]
Adjaye, J, Marsh, P., Eagles, P. (1993). Immunological properties and cDNA sequence analysis of an intermediate-filament-like protein from squid neuronal tissue. J. Cell Sci. 106: 1283-1290 [Abstract]
Bossie, C., Sanders, M. (1993). A cDNA from Drosophila melanogaster encodes a lamin C-like intermediate filament protein. J. Cell Sci. 104: 1263-1272 [Abstract]
Lourim, D., Lin, J. J. (1992). Expression of wild-type and nuclear localization-deficient human lamin A in chick myogenic cells. J. Cell Sci. 103: 863-874 [Abstract]
Lin, H., Song, K., Hutcheson, G.A., Goutte, C.E., Wolfner, M.F. (1991). A Maternally Encoded Nuclear Envelope Protein Required for Embryonic Mitosis in Drosophila. Cold Spring Harb Symp Quant Biol 56: 719-727 [Abstract]
Schafer, W., Kim, R, Sterne, R, Thorner, J, Kim, S., Rine, J (1989). Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans. Science 245: 379-385 [Abstract]