Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line

doi:10.1083/jcb.106.3.761

This Article

	Full Text (PDF, 3862K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Boukamp, P.
	Articles by Fusenig, N. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Boukamp, P.
	Articles by Fusenig, N. E.


Social Bookmarking

	What's this?

ARTICLES

Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line

P Boukamp, RT Petrussevska, D Breitkreutz, J Hornung, A Markham and NE Fusenig
Division of Differentiation and Carcinogenesis in Vitro, German Cancer Research Center, Heidelberg.

In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation- specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long- term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Koinuma, D., Tsutsumi, S., Kamimura, N., Taniguchi, H., Miyazawa, K., Sunamura, M., Imamura, T., Miyazono, K., Aburatani, H. (2009). Chromatin Immunoprecipitation on Microarray Analysis of Smad2/3 Binding Sites Reveals Roles of ETS1 and TFAP2A in Transforming Growth Factor {beta} Signaling. Mol. Cell. Biol. 29: 172-186 [Abstract] [Full Text]
Schmidt, E., Gutberlet, J., Siegmund, D., Berg, D., Wajant, H., Waschke, J. (2009). Apoptosis is not required for acantholysis in pemphigus vulgaris. Am. J. Physiol. Cell Physiol. 296: C162-C172 [Abstract] [Full Text]
Smith, J. L., Campos, S. K., Wandinger-Ness, A., Ozbun, M. A. (2008). Caveolin-1-Dependent Infectious Entry of Human Papillomavirus Type 31 in Human Keratinocytes Proceeds to the Endosomal Pathway for pH-Dependent Uncoating. J. Virol. 82: 9505-9512 [Abstract] [Full Text]
Rosenbluth, J. M., Mays, D. J., Pino, M. F., Tang, L. J., Pietenpol, J. A. (2008). A Gene Signature-Based Approach Identifies mTOR as a Regulator of p73. Mol. Cell. Biol. 28: 5951-5964 [Abstract] [Full Text]
Olson, E. R., Melton, T., Dong, Z., Bowden, G. T. (2008). Stabilization of Quercetin Paradoxically Reduces Its Proapoptotic Effect on UVB-Irradiated Human Keratinocytes. Cancer Prevention Research 1: 362-368 [Abstract] [Full Text]
Schiefelbein, D., Seitz, O., Goren, I., Dissmann, J. P., Schmidt, H., Bachmann, M., Sader, R., Geisslinger, G., Pfeilschifter, J., Frank, S. (2008). Keratinocyte-Derived Vascular Endothelial Growth Factor Biosynthesis Represents a Pleiotropic Side Effect of Peroxisome Proliferator-Activated Receptor-{gamma} Agonist Troglitazone but Not Rosiglitazone and Involves Activation of p38 Mitogen-Activated Protein Kinase: Implications for Diabetes-Impaired Skin Repair. Mol. Pharmacol. 74: 952-963 [Abstract] [Full Text]
Fitsialos, G., Bourget, I., Augier, S., Ginouves, A., Rezzonico, R., Odorisio, T., Cianfarani, F., Virolle, T., Pouyssegur, J., Meneguzzi, G., Berra, E., Ponzio, G., Busca, R. (2008). HIF1 transcription factor regulates laminin-332 expression and keratinocyte migration. J. Cell Sci. 121: 2992-3001 [Abstract] [Full Text]
Lai, K.-C., Chang, K.-W., Liu, C.-J., Kao, S.-Y., Lee, T.-C. (2008). IFN-Induced Protein with Tetratricopeptide Repeats 2 Inhibits Migration Activity and Increases Survival of Oral Squamous Cell Carcinoma. Mol Cancer Res 6: 1431-1439 [Abstract] [Full Text]
Tanese, K., Fukuma, M., Yamada, T., Mori, T., Yoshikawa, T., Watanabe, W., Ishiko, A., Amagai, M., Nishikawa, T., Sakamoto, M. (2008). G-Protein-Coupled Receptor GPR49 is Up-regulated in Basal Cell Carcinoma and Promotes Cell Proliferation and Tumor Formation. Am. J. Pathol. 173: 835-843 [Abstract] [Full Text]
Reichard, J. F., Sartor, M. A., Puga, A. (2008). BACH1 Is a Specific Repressor of HMOX1 That Is Inactivated by Arsenite. J. Biol. Chem. 283: 22363-22370 [Abstract] [Full Text]
Tse, W.-P., Cheng, C. H. K., Che, C.-T., Lin, Z.-X. (2008). Arsenic Trioxide, Arsenic Pentoxide, and Arsenic Iodide Inhibit Human Keratinocyte Proliferation through the Induction of Apoptosis. J. Pharmacol. Exp. Ther. 326: 388-394 [Abstract] [Full Text]
Murzik, U., Hemmerich, P., Weidtkamp-Peters, S., Ulbricht, T., Bussen, W., Hentschel, J., von Eggeling, F., Melle, C. (2008). Rad54B Targeting to DNA Double-Strand Break Repair Sites Requires Complex Formation with S100A11. Mol. Biol. Cell 19: 2926-2935 [Abstract] [Full Text]
Luke, Y., Zaim, H., Karakesisoglou, I., Jaeger, V. M., Sellin, L., Lu, W., Schneider, M., Neumann, S., Beijer, A., Munck, M., Padmakumar, V. C., Gloy, J., Walz, G., Noegel, A. A. (2008). Nesprin-2 Giant (NUANCE) maintains nuclear envelope architecture and composition in skin. J. Cell Sci. 121: 1887-1898 [Abstract] [Full Text]
Schiefelbein, D., Goren, I., Fisslthaler, B., Schmidt, H., Geisslinger, G., Pfeilschifter, J., Frank, S. (2008). Biphasic Regulation of HMG-CoA Reductase Expression and Activity during Wound Healing and Its Functional Role in the Control of Keratinocyte Angiogenic and Proliferative Responses. J. Biol. Chem. 283: 15479-15490 [Abstract] [Full Text]
He, Y.-Y., Council, S. E., Feng, L., Chignell, C. F. (2008). UVA-Induced Cell Cycle Progression Is Mediated by a Disintegrin and Metalloprotease/Epidermal Growth Factor Receptor/AKT/Cyclin D1 Pathways in Keratinocytes. Cancer Res. 68: 3752-3758 [Abstract] [Full Text]
Lanza, A., Cirillo, N., Rossiello, R., Rienzo, M., Cutillo, L., Casamassimi, A., de Nigris, F., Schiano, C., Rossiello, L., Femiano, F., Gombos, F., Napoli, C. (2008). Evidence of Key Role of Cdk2 Overexpression in Pemphigus Vulgaris. J. Biol. Chem. 283: 8736-8745 [Abstract] [Full Text]
Bhagavathula, N., Nerusu, K. C., Hanosh, A., Aslam, M. N., Sundberg, T. B., Opipari, A. W. Jr., Johnson, K., Kang, S., Glick, G. D., Varani, J. (2008). 7-Chloro-5-(4-hydroxyphenyl)-1-methyl-3-(naphthalen-2-ylmethyl)-4,5-dihydro-1H-benzo[b][1,4]diazepin-2(3H)-one (Bz-423), a Benzodiazepine, Suppresses Keratinocyte Proliferation and Has Antipsoriatic Activity in the Human Skin-Severe, Combined Immunodeficient Mouse Transplant Model. J. Pharmacol. Exp. Ther. 324: 938-947 [Abstract] [Full Text]
Gallegos, J. R., Litersky, J., Lee, H., Sun, Y., Nakayama, K., Nakayama, K., Lu, H. (2008). SCF TrCP1 Activates and Ubiquitylates TAp63{gamma}. J. Biol. Chem. 283: 66-75 [Abstract] [Full Text]
Bazhin, A. V., Schadendorf, D., Owen, R. W., Zernii, E. Y., Philippov, P. P., Eichmuller, S. B. (2008). Visible Light Modulates the Expression of Cancer-Retina Antigens. Mol Cancer Res 6: 110-118 [Abstract] [Full Text]
Reichard, J. F., Motz, G. T., Puga, A. (2007). Heme oxygenase-1 induction by NRF2 requires inactivation of the transcriptional repressor BACH1. Nucleic Acids Res 35: 7074-7086 [Abstract] [Full Text]
Adamiak, B., Ekblad, M., Bergstrom, T., Ferro, V., Trybala, E. (2007). Herpes Simplex Virus Type 2 Glycoprotein G Is Targeted by the Sulfated Oligo- and Polysaccharide Inhibitors of Virus Attachment to Cells. J. Virol. 81: 13424-13434 [Abstract] [Full Text]
Taboubi, S., Milanini, J., Delamarre, E., Parat, F., Garrouste, F., Pommier, G., Takasaki, J., Hubaud, J.-C., Kovacic, H., Lehmann, M. (2007). G{alpha}(q/11)-coupled P2Y2 nucleotide receptor inhibits human keratinocyte spreading and migration. FASEB J. 21: 4047-4058 [Abstract] [Full Text]
Smith, J. L., Campos, S. K., Ozbun, M. A. (2007). Human Papillomavirus Type 31 Uses a Caveolin 1- and Dynamin 2-Mediated Entry Pathway for Infection of Human Keratinocytes. J. Virol. 81: 9922-9931 [Abstract] [Full Text]
Fukuda, M., Longnecker, R. (2007). Epstein-Barr Virus Latent Membrane Protein 2A Mediates Transformation through Constitutive Activation of the Ras/PI3-K/Akt Pathway. J. Virol. 81: 9299-9306 [Abstract] [Full Text]
Lehen'kyi, V., Beck, B., Polakowska, R., Charveron, M., Bordat, P., Skryma, R., Prevarskaya, N. (2007). TRPV6 Is a Ca2+ Entry Channel Essential for Ca2+-induced Differentiation of Human Keratinocytes. J. Biol. Chem. 282: 22582-22591 [Abstract] [Full Text]
Ekman, A., Hokynar, K., Kakkola, L., Kantola, K., Hedman, L., Bonden, H., Gessner, M., Aberham, C., Norja, P., Miettinen, S., Hedman, K., Soderlund-Venermo, M. (2007). Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3. J. Virol. 81: 6927-6935 [Abstract] [Full Text]
Patel, V., Rosenfeldt, H. M., Lyons, R., Servitja, J.-M., Bustelo, X. R., Siroff, M., Gutkind, J.S. (2007). Persistent activation of Rac1 in squamous carcinomas of the head and neck: evidence for an EGFR/Vav2 signaling axis involved in cell invasion. Carcinogenesis 28: 1145-1152 [Abstract] [Full Text]
Storey, A., Rogers, J. S., McArdle, F., Jackson, M. J., Rhodes, L. E. (2007). Conjugated linoleic acids modulate UVR-induced IL-8 and PGE2 in human skin cells: potential of CLA isomers in nutritional photoprotection. Carcinogenesis 28: 1329-1333 [Abstract] [Full Text]
Fritsche, E., Schafer, C., Calles, C., Bernsmann, T., Bernshausen, T., Wurm, M., Hubenthal, U., Cline, J. E., Hajimiragha, H., Schroeder, P., Klotz, L.-O., Rannug, A., Furst, P., Hanenberg, H., Abel, J., Krutmann, J. (2007). Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiation. Proc. Natl. Acad. Sci. USA 104: 8851-8856 [Abstract] [Full Text]
Wan, H., Yuan, M., Simpson, C., Allen, K., Gavins, F. N.E., Ikram, M. S., Basu, S., Baksh, N., O'Toole, E. A., Hart, I. R. (2007). Stem/Progenitor Cell-Like Properties of Desmoglein 3dim Cells in Primary and Immortalized Keratinocyte Lines. Stem Cells 25: 1286-1297 [Abstract] [Full Text]
Ogawa, T., Tsubota, Y., Hashimoto, J., Kariya, Y., Miyazaki, K. (2007). The Short Arm of Laminin {gamma}2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin beta4 Chain. Mol. Biol. Cell 18: 1621-1633 [Abstract] [Full Text]
Saavalainen, K., Tammi, M. I., Bowen, T., Schmitz, M. L., Carlberg, C. (2007). Integration of the Activation of the Human Hyaluronan Synthase 2 Gene Promoter by Common Cofactors of the Transcription Factors Retinoic Acid Receptor and Nuclear Factor {kappa}B. J. Biol. Chem. 282: 11530-11539 [Abstract] [Full Text]
Minsavage, G. D., Dillman, J. F. III (2007). Bifunctional Alkylating Agent-Induced p53 and Nonclassical Nuclear Factor {kappa}B Responses and Cell Death Are Altered by Caffeic Acid Phenethyl Ester: A Potential Role for Antioxidant/Electrophilic Response-Element Signaling. J. Pharmacol. Exp. Ther. 321: 202-212 [Abstract] [Full Text]
Ardern-Jones, M. R., Black, A. P., Bateman, E. A., Ogg, G. S. (2007). Bacterial superantigen facilitates epithelial presentation of allergen to T helper 2 cells. Proc. Natl. Acad. Sci. USA 104: 5557-5562 [Abstract] [Full Text]
Hurgin, V., Novick, D., Werman, A., Dinarello, C. A., Rubinstein, M. (2007). Antiviral and immunoregulatory activities of IFN-{gamma} depend on constitutively expressed IL-1{alpha}. Proc. Natl. Acad. Sci. USA 104: 5044-5049 [Abstract] [Full Text]
Tellam, J., Fogg, M. H., Rist, M., Connolly, G., Tscharke, D., Webb, N., Heslop, L., Wang, F., Khanna, R. (2007). Influence of translation efficiency of homologous viral proteins on the endogenous presentation of CD8+ T cell epitopes. JEM 204: 525-532 [Abstract] [Full Text]
Rosenberger, S., Thorey, I. S., Werner, S., Boukamp, P. (2007). A Novel Regulator of Telomerase: S100A8 MEDIATES DIFFERENTIATION-DEPENDENT AND CALCIUM-INDUCED INHIBITION OF TELOMERASE ACTIVITY IN THE HUMAN EPIDERMAL KERATINOCYTE LINE HaCaT. J. Biol. Chem. 282: 6126-6135 [Abstract] [Full Text]
Willhauck, M. J., Mirancea, N., Vosseler, S., Pavesio, A., Boukamp, P., Mueller, M. M., Fusenig, N. E., Stark, H.-J. (2007). Reversion of tumor phenotype in surface transplants of skin SCC cells by scaffold-induced stroma modulation. Carcinogenesis 28: 595-610 [Abstract] [Full Text]
Takano, S., Kanai, F., Jazag, A., Ijichi, H., Yao, J., Ogawa, H., Enomoto, N., Omata, M., Nakao, A. (2007). Smad4 is Essential for Down-regulation of E-cadherin Induced by TGF-{beta} in Pancreatic Cancer Cell Line PANC-1. J Biochem 141: 345-351 [Abstract] [Full Text]
Farnsworth, A., Wisner, T. W., Johnson, D. C. (2007). Cytoplasmic Residues of Herpes Simplex Virus Glycoprotein gE Required for Secondary Envelopment and Binding of Tegument Proteins VP22 and UL11 to gE and gD. J. Virol. 81: 319-331 [Abstract] [Full Text]
Deyrieux, A. F., Rosas-Acosta, G., Ozbun, M. A., Wilson, V. G. (2007). Sumoylation dynamics during keratinocyte differentiation. J. Cell Sci. 120: 125-136 [Abstract] [Full Text]
Eidsmo, L., Fluur, C., Rethi, B., Eriksson Ygberg, S., Ruffin, N., De Milito, A., Akuffo, H., Chiodi, F. (2007). FasL and TRAIL Induce Epidermal Apoptosis and Skin Ulceration Upon Exposure to Leishmania major. Am. J. Pathol. 170: 227-239 [Abstract] [Full Text]
Mendoza, J.-A., Jacob, Y., Cassonnet, P., Favre, M. (2006). Human Papillomavirus Type 5 E6 Oncoprotein Represses the Transforming Growth Factor {beta} Signaling Pathway by Binding to SMAD3. J. Virol. 80: 12420-12424 [Abstract] [Full Text]
Andersson, A.-C., Stromberg, S., Backvall, H., Kampf, C., Uhlen, M., Wester, K., Ponten, F. (2006). Analysis of Protein Expression in Cell Microarrays: A Tool for Antibody-based Proteomics. J. Histochem. Cytochem. 54: 1413-1423 [Abstract] [Full Text]
Braun, S., Krampert, M., Bodo, E., Kumin, A., Born-Berclaz, C., Paus, R., Werner, S. (2006). Keratinocyte growth factor protects epidermis and hair follicles from cell death induced by UV irradiation, chemotherapeutic or cytotoxic agents. J. Cell Sci. 119: 4841-4849 [Abstract] [Full Text]
Lederle, W., Stark, H.-J., Skobe, M., Fusenig, N. E., Mueller, M. M. (2006). Platelet-Derived Growth Factor-BB Controls Epithelial Tumor Phenotype by Differential Growth Factor Regulation in Stromal Cells. Am. J. Pathol. 169: 1767-1783 [Abstract] [Full Text]
Sitailo, L. A., Tibudan, S. S., Denning, M. F. (2006). The Protein Kinase C{delta} Catalytic Fragment Targets Mcl-1 for Degradation to Trigger Apoptosis. J. Biol. Chem. 281: 29703-29710 [Abstract] [Full Text]
Pankow, S., Bamberger, C., Klippel, A., Werner, S. (2006). Regulation of epidermal homeostasis and repair by phosphoinositide 3-kinase. J. Cell Sci. 119: 4033-4046 [Abstract] [Full Text]
Pani, B., Cornatzer, E., Cornatzer, W., Shin, D.-M., Pittelkow, M. R., Hovnanian, A., Ambudkar, I. S., Singh, B. B. (2006). Up-Regulation of Transient Receptor Potential Canonical 1 (TRPC1) following Sarco(endo)plasmic Reticulum Ca2+ ATPase 2 Gene Silencing Promotes Cell Survival: A Potential Role for TRPC1 in Darier's Disease. Mol. Biol. Cell 17: 4446-4458 [Abstract] [Full Text]
van Rietschoten, J. G. I., Verzijlbergen, K. F., Gringhuis, S. I., Kraan, T. C. T. M. v. d. P., Bayley, J.-P., Wierenga, E. A., Jones, P. A., Kooter, J. M., Verweij, C. L. (2006). Differentially methylated alleles in a distinct region of the human interleukin-1{alpha} promoter are associated with allele-specific expression of IL-1{alpha} in CD4+ T cells. Blood 108: 2143-2149 [Abstract] [Full Text]
Culp, T. D., Budgeon, L. R., Marinkovich, M. P., Meneguzzi, G., Christensen, N. D. (2006). Keratinocyte-secreted laminin 5 can function as a transient receptor for human papillomaviruses by binding virions and transferring them to adjacent cells.. J. Virol. 80: 8940-8950 [Abstract] [Full Text]
Rossi, M., Aqeilan, R. I., Neale, M., Candi, E., Salomoni, P., Knight, R. A., Croce, C. M., Melino, G. (2006). The E3 ubiquitin ligase Itch controls the protein stability of p63. Proc. Natl. Acad. Sci. USA 103: 12753-12758 [Abstract] [Full Text]
Kasper, M., Schnidar, H., Neill, G. W., Hanneder, M., Klingler, S., Blaas, L., Schmid, C., Hauser-Kronberger, C., Regl, G., Philpott, M. P., Aberger, F. (2006). Selective Modulation of Hedgehog/GLI Target Gene Expression by Epidermal Growth Factor Signaling in Human Keratinocytes.. Mol. Cell. Biol. 26: 6283-6298 [Abstract] [Full Text]
Barbieri, C. E., Tang, L. J., Brown, K. A., Pietenpol, J. A. (2006). Loss of p63 Leads to Increased Cell Migration and Up-regulation of Genes Involved in Invasion and Metastasis.. Cancer Res. 66: 7589-7597 [Abstract] [Full Text]
Hakulinen, J., Keski-Oja, J. (2006). ADAM10-mediated Release of Complement Membrane Cofactor Protein during Apoptosis of Epithelial Cells. J. Biol. Chem. 281: 21369-21376 [Abstract] [Full Text]
Basile, J. R., Castilho, R. M., Williams, V. P., Gutkind, J. S. (2006). Semaphorin 4D provides a link between axon guidance processes and tumor-induced angiogenesis. Proc. Natl. Acad. Sci. USA 103: 9017-9022 [Abstract] [Full Text]
Ren, Q., Kari, C., Quadros, M. R.D., Burd, R., McCue, P., Dicker, A. P., Rodeck, U. (2006). Malignant Transformation of Immortalized HaCaT Keratinocytes through Deregulated Nuclear Factor {kappa}B Signaling.. Cancer Res. 66: 5209-5215 [Abstract] [Full Text]
Fulcher, R. A., Cole, L. E., Janowicz, D. M., Toffer, K. L., Fortney, K. R., Katz, B. P., Orndorff, P. E., Spinola, S. M., Kawula, T. H. (2006). Expression of Haemophilus ducreyi Collagen Binding Outer Membrane Protein NcaA Is Required for Virulence in Swine and Human Challenge Models of Chancroid.. Infect. Immun. 74: 2651-2658 [Abstract] [Full Text]
Ludwig, H., Suezer, Y., Waibler, Z., Kalinke, U., Schnierle, B. S., Sutter, G. (2006). Double-stranded RNA-binding protein E3 controls translation of viral intermediate RNA, marking an essential step in the life cycle of modified vaccinia virus Ankara.. J. Gen. Virol. 87: 1145-1155 [Abstract] [Full Text]
Lu, J., Lin, W.-H., Chen, S.-Y., Longnecker, R., Tsai, S.-C., Chen, C.-L., Tsai, C.-H. (2006). Syk Tyrosine Kinase Mediates Epstein-Barr Virus Latent Membrane Protein 2A-induced Cell Migration in Epithelial Cells. J. Biol. Chem. 281: 8806-8814 [Abstract] [Full Text]
Katagiri, C., Nakanishi, J., Kadoya, K., Hibino, T. (2006). Serpin squamous cell carcinoma antigen inhibits UV-induced apoptosis via suppression of c-JUN NH2-terminal kinase. JCB 172: 983-990 [Abstract] [Full Text]
Morley, N., Rapp, A., Dittmar, H., Salter, L., Gould, D., Greulich, K.O., Curnow, A. (2006). UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells. Mutagenesis 21: 105-114 [Abstract] [Full Text]
Bouez, C., Reynaud, C., Noblesse, E., Thepot, A., Gleyzal, C., Kanitakis, J., Perrier, E., Damour, O., Sommer, P. (2006). The Lysyl Oxidase LOX Is Absent in Basal and Squamous Cell Carcinomas and Its Knockdown Induces an Invading Phenotype in a Skin Equivalent Model. Clin. Cancer Res. 12: 1463-1469 [Abstract] [Full Text]
Hofmann, I., Casella, M., Schnolzer, M., Schlechter, T., Spring, H., Franke, W. W. (2006). Identification of the Junctional Plaque Protein Plakophilin 3 in Cytoplasmic Particles Containing RNA-binding Proteins and the Recruitment of Plakophilins 1 and 3 to Stress Granules. Mol. Biol. Cell 17: 1388-1398 [Abstract] [Full Text]
Sher, I., Zisman-Rozen, S., Eliahu, L., Whitelock, J. M., Maas-Szabowski, N., Yamada, Y., Breitkreutz, D., Fusenig, N. E., Arikawa-Hirasawa, E., Iozzo, R. V., Bergman, R., Ron, D. (2006). Targeting Perlecan in Human Keratinocytes Reveals Novel Roles for Perlecan in Epidermal Formation. J. Biol. Chem. 281: 5178-5187 [Abstract] [Full Text]
Fishelevich, R., Malanina, A., Luzina, I., Atamas, S., Smyth, M. J., Porcelli, S. A., Gaspari, A. A. (2006). Ceramide-Dependent Regulation of Human Epidermal Keratinocyte CD1d Expression during Terminal Differentiation. J. Immunol. 176: 2590-2599 [Abstract] [Full Text]
Dusek, R. L., Getsios, S., Chen, F., Park, J. K., Amargo, E. V., Cryns, V. L., Green, K. J. (2006). The Differentiation-dependent Desmosomal Cadherin Desmoglein 1 Is a Novel Caspase-3 Target That Regulates Apoptosis in Keratinocytes. J. Biol. Chem. 281: 3614-3624 [Abstract] [Full Text]
Kurschat, P., Bielenberg, D., Rossignol-Tallandier, M., Stahl, A., Klagsbrun, M. (2006). Neuron Restrictive Silencer Factor NRSF/REST Is a Transcriptional Repressor of Neuropilin-1 and Diminishes the Ability of Semaphorin 3A to Inhibit Keratinocyte Migration. J. Biol. Chem. 281: 2721-2729 [Abstract] [Full Text]
Takeda, S., Mitsutake, S., Tsuji, K., Igarashi, Y. (2006). Apoptosis Occurs via the Ceramide Recycling Pathway in Human HaCaT Keratinocytes. J Biochem 139: 255-262 [Abstract] [Full Text]
Rockel, T. D., Stuhlmann, D., von Mikecz, A. (2005). Proteasomes degrade proteins in focal subdomains of the human cell nucleus. J. Cell Sci. 118: 5231-5242 [Abstract] [Full Text]
Tanaka, K., Hasegawa, J., Asamitsu, K., Okamoto, T. (2005). Prevention of the Ultraviolet B-Mediated Skin Photoaging by a Nuclear Factor {kappa}B Inhibitor, Parthenolide. J. Pharmacol. Exp. Ther. 315: 624-630 [Abstract] [Full Text]
Miller, D. W., Vosseler, S., Mirancea, N., Hicklin, D. J., Bohlen, P., Volcker, H. E., Holz, F. G., Fusenig, N. E. (2005). Rapid Vessel Regression, Protease Inhibition, and Stromal Normalization upon Short-Term Vascular Endothelial Growth Factor Receptor 2 Inhibition in Skin Carcinoma Heterotransplants. Am. J. Pathol. 167: 1389-1403 [Abstract] [Full Text]
Boukamp, P. (2005). Non-melanoma skin cancer: what drives tumor development and progression?. Carcinogenesis 26: 1657-1667 [Abstract] [Full Text]
Lee, E.-R., Kang, Y.-J., Kim, J.-H., Lee, H. T., Cho, S.-G. (2005). Modulation of Apoptosis in HaCaT Keratinocytes via Differential Regulation of ERK Signaling Pathway by Flavonoids. J. Biol. Chem. 280: 31498-31507 [Abstract] [Full Text]
Burrell, H. E., Wlodarski, B., Foster, B. J., Buckley, K. A., Sharpe, G. R., Quayle, J. M., Simpson, A. W. M., Gallagher, J. A. (2005). Human Keratinocytes Release ATP and Utilize Three Mechanisms for Nucleotide Interconversion at the Cell Surface. J. Biol. Chem. 280: 29667-29676 [Abstract] [Full Text]
Ledirac, N., Antherieu, S., d'Uby, A. D., Caron, J.-C., Rahmani, R. (2005). Effects of Organochlorine Insecticides on MAP Kinase Pathways in Human HaCaT Keratinocytes: Key Role of Reactive Oxygen Species. Toxicol Sci 86: 444-452 [Abstract] [Full Text]
Pini, A., Giuliani, A., Falciani, C., Runci, Y., Ricci, C., Lelli, B., Malossi, M., Neri, P., Rossolini, G. M., Bracci, L. (2005). Antimicrobial Activity of Novel Dendrimeric Peptides Obtained by Phage Display Selection and Rational Modification. Antimicrob. Agents Chemother. 49: 2665-2672 [Abstract] [Full Text]
Maretzky, T., Reiss, K., Ludwig, A., Buchholz, J., Scholz, F., Proksch, E., de Strooper, B., Hartmann, D., Saftig, P. (2005). ADAM10 mediates E-cadherin shedding and regulates epithelial cell-cell adhesion, migration, and {beta}-catenin translocation. Proc. Natl. Acad. Sci. USA 102: 9182-9187 [Abstract] [Full Text]
Pyeon, D., Lambert, P. F., Ahlquist, P. (2005). Production of infectious human papillomavirus independently of viral replication and epithelial cell differentiation. Proc. Natl. Acad. Sci. USA 102: 9311-9316 [Abstract] [Full Text]
Krampert, M., Kuenzle, S., Thai, S. N.-M., Lee, N., Iruela-Arispe, M. L., Werner, S. (2005). ADAMTS1 Proteinase Is Up-regulated in Wounded Skin and Regulates Migration of Fibroblasts and Endothelial Cells. J. Biol. Chem. 280: 23844-23852 [Abstract] [Full Text]
Nicola, A. V., Hou, J., Major, E. O., Straus, S. E. (2005). Herpes Simplex Virus Type 1 Enters Human Epidermal Keratinocytes, but Not Neurons, via a pH-Dependent Endocytic Pathway. J. Virol. 79: 7609-7616 [Abstract] [Full Text]
Patterson, N. A., Smith, J. L., Ozbun, M. A. (2005). Human Papillomavirus Type 31b Infection of Human Keratinocytes Does Not Require Heparan Sulfate. J. Virol. 79: 6838-6847 [Abstract] [Full Text]
Becker, D., Blase, C., Bereiter-Hahn, J., Jendrach, M. (2005). TRPV4 exhibits a functional role in cell-volume regulation. J. Cell Sci. 118: 2435-2440 [Abstract] [Full Text]
Smola-Hess, S., Pahne, J., Mauch, C., Zigrino, P., Smola, H., Pfister, H. J. (2005). Expression of membrane type 1 matrix metalloproteinase in papillomavirus-positive cells: role of the human papillomavirus (HPV) 16 and HPV8 E7 gene products. J. Gen. Virol. 86: 1291-1296 [Abstract] [Full Text]
Baghdoyan, S., Lamartine, J., Castel, D., Pitaval, A., Roupioz, Y., Franco, N., Duarte, M., Martin, M. T., Gidrol, X. (2005). Id2 Reverses Cell Cycle Arrest Induced by {gamma}-Irradiation in Human HaCaT Keratinocytes. J. Biol. Chem. 280: 15836-15841 [Abstract] [Full Text]
Rubio, D., Garcia-Castro, J., Martin, M. C., de la Fuente, R., Cigudosa, J. C., Lloyd, A. C., Bernad, A. (2005). Spontaneous Human Adult Stem Cell Transformation. Cancer Res. 65: 3035-3039 [Abstract] [Full Text]
Tsubota, Y., Yasuda, C., Kariya, Y., Ogawa, T., Hirosaki, T., Mizushima, H., Miyazaki, K. (2005). Regulation of Biological Activity and Matrix Assembly of Laminin-5 by COOH-terminal, LG4-5 Domain of {alpha}3 Chain. J. Biol. Chem. 280: 14370-14377 [Abstract] [Full Text]
Saavalainen, K., Pasonen-Seppanen, S., Dunlop, T. W., Tammi, R., Tammi, M. I., Carlberg, C. (2005). The Human Hyaluronan Synthase 2 Gene Is a Primary Retinoic Acid and Epidermal Growth Factor Responding Gene. J. Biol. Chem. 280: 14636-14644 [Abstract] [Full Text]
Holmgren, S. C., Patterson, N. A., Ozbun, M. A., Lambert, P. F. (2005). The Minor Capsid Protein L2 Contributes to Two Steps in the Human Papillomavirus Type 31 Life Cycle. J. Virol. 79: 3938-3948 [Abstract] [Full Text]
Eidsmo, L., Nylen, S., Khamesipour, A., Hedblad, M.-A., Chiodi, F., Akuffo, H. (2005). The Contribution of the Fas/FasL Apoptotic Pathway in Ulcer Formation during Leishmania major-Induced Cutaneous Leishmaniasis. Am. J. Pathol. 166: 1099-1108 [Abstract] [Full Text]
Meissner, M., Reichert, T. E., Kunkel, M., Gooding, W., Whiteside, T. L., Ferrone, S., Seliger, B. (2005). Defects in the Human Leukocyte Antigen Class I Antigen Processing Machinery in Head and Neck Squamous Cell Carcinoma: Association with Clinical Outcome. Clin. Cancer Res. 11: 2552-2560 [Abstract] [Full Text]
Farina, A., Feederle, R., Raffa, S., Gonnella, R., Santarelli, R., Frati, L., Angeloni, A., Torrisi, M. R., Faggioni, A., Delecluse, H.-J. (2005). BFRF1 of Epstein-Barr Virus Is Essential for Efficient Primary Viral Envelopment and Egress. J. Virol. 79: 3703-3712 [Abstract] [Full Text]
Hintermann, E., Yang, N., O'Sullivan, D., Higgins, J. M. G., Quaranta, V. (2005). Integrin {alpha}6{beta}4-erbB2 Complex Inhibits Haptotaxis by Up-regulating E-cadherin Cell-Cell Junctions in Keratinocytes. J. Biol. Chem. 280: 8004-8015 [Abstract] [Full Text]
Satish, L., Blair, H. C., Glading, A., Wells, A. (2005). Interferon-Inducible Protein 9 (CXCL11)-Induced Cell Motility in Keratinocytes Requires Calcium Flux-Dependent Activation of {micro}-Calpain. Mol. Cell. Biol. 25: 1922-1941 [Abstract] [Full Text]
Morrison, J. A., Raab-Traub, N. (2005). Roles of the ITAM and PY Motifs of Epstein-Barr Virus Latent Membrane Protein 2A in the Inhibition of Epithelial Cell Differentiation and Activation of {beta}-Catenin Signaling. J. Virol. 79: 2375-2382 [Abstract] [Full Text]
Vosseler, S., Mirancea, N., Bohlen, P., Mueller, M. M., Fusenig, N. E. (2005). Angiogenesis Inhibition by Vascular Endothelial Growth Factor Receptor-2 Blockade Reduces Stromal Matrix Metalloproteinase Expression, Normalizes Stromal Tissue, and Reverts Epithelial Tumor Phenotype in Surface Heterotransplants. Cancer Res. 65: 1294-1305 [Abstract] [Full Text]
Allen, M. D., Young, L. S., Dawson, C. W. (2005). The Epstein-Barr Virus-Encoded LMP2A and LMP2B Proteins Promote Epithelial Cell Spreading and Motility. J. Virol. 79: 1789-1802 [Abstract] [Full Text]