Differential effects of laminin, intact type IV collagen, and specific domains of type IV collagen on endothelial cell adhesion and migration

doi:10.1083/jcb.106.4.1365

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Differential effects of laminin, intact type IV collagen, and specific domains of type IV collagen on endothelial cell adhesion and migration

TJ Herbst, JB McCarthy, EC Tsilibary and LT Furcht
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.

Laminin and type IV collagen were compared for the ability to promote aortic endothelial cell adhesion and directed migration in vitro. Substratum-adsorbed IV promoted aortic endothelial cell adhesion in a concentration dependent fashion attaining a maximum level 141-fold greater than controls within 30 min. Aortic endothelial cell adhesion to type IV collagen was not inhibited by high levels (10(-3) M) of arginyl-glycyl-aspartyl-serine. In contrast, adhesion of aortic endothelial cells on laminin was slower, attaining only 53% of the adhesion observed on type IV collagen by 90 min. Type IV collagen when added to the lower well of a Boyden chamber stimulated the directional migration of aortic endothelial cells in a concentration dependent manner with a maximal response 6.9-fold over control levels, whereas aortic endothelial cells did not migrate in response to laminin at any concentration (.01-2.0 X 10(-7) M). Triple helix-rich fragments of type IV collagen were nearly as active as intact type IV collagen in stimulating both adhesion and migration whereas the carboxy terminal globular domain was less active at promoting adhesion (36% of the adhesion promoted by intact type IV collagen) or migration. Importantly, aortic endothelial cells also migrate to substratum adsorbed gradients of type IV collagen suggesting that the mechanism of migration is haptotactic in nature. These results demonstrate that the aortic endothelial cell adhesion and migration is preferentially promoted by type IV collagen compared with laminin, and has a complex molecular basis which may be important in angiogenesis and large vessel repair.
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Li, S., Butler, P., Wang, Y., Hu, Y., Han, D. C., Usami, S., Guan, J.-L., Chien, S. (2002). The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells. Proc. Natl. Acad. Sci. USA 99: 3546-3551 [Abstract] [Full Text]
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Keely, P., Fong, A., Zutter, M., Santoro, S. (1995). Alteration of collagen-dependent adhesion, motility, and morphogenesis by the expression of antisense alpha 2 integrin mRNA in mammary cells. J. Cell Sci. 108: 595-607 [Abstract]
Perris, R, Syfrig, J, Paulsson, M, Bronner-Fraser, M (1993). Molecular mechanisms of neural crest cell attachment and migration on types I and IV collagen. J. Cell Sci. 106: 1357-1368 [Abstract]
Faassen, A., Mooradian, D., Tranquillo, R., Dickinson, R., Letourneau, P., Oegema, T., McCarthy, J. (1993). Cell surface CD44-related chondroitin sulfate proteoglycan is required for transforming growth factor-beta-stimulated mouse melanoma cell motility and invasive behavior on type I collagen. J. Cell Sci. 105: 501-511 [Abstract]
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Greisler, H. P., Johnson, S., Joyce, K., Henderson, S., Patel, N. M., Alkhamis, T., Beissinger, R., Kim, D. U. (1990). The Effects of Shear Stress on Endothelial Cell Retention and Function on Expanded Polytetrafluoroethylene. Arch Surg 125: 1622-1625 [Abstract]