A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts

doi:10.1083/jcb.109.6.2575

Get More Out of Microscopy - Agilent iMIC 2000

This Article

	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pinol-Roma, S.
	Articles by Dreyfuss, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pinol-Roma, S.
	Articles by Dreyfuss, G.


Social Bookmarking

	What's this?

ARTICLES

A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts

S Pinol-Roma, MS Swanson, JG Gall and G Dreyfuss
Department of Biochemistry, Molecular Biology, Northwestern University, Evanston, Illinois 60208.

Immediately after the initiation of transcription in eukaryotes, nascent RNA polymerase II transcripts are bound by nuclear proteins resulting in the formation of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. hnRNP complexes from HeLa cell nuclei contain greater than 20 major proteins in the molecular mass range of 34,000- 120,000 D. Among these are the previously described A, B, and C groups of proteins (34,000-43,000 D) and several larger, and as yet uncharacterized, proteins. Here we describe the isolation and characterization of a novel hnRNP protein termed the L protein (64-68 kD by mobility in SDS-polyacrylamide gels). Although L is a bona fide component of hnRNP complexes, it also appears to be a different type of hnRNP protein from those previously characterized. A considerable amount of L is found outside hnRNP complexes, and monoclonal antibodies to the L protein also strongly stain unidentified discrete nonnucleolar structures, in addition to nucleoplasm, in HeLa cell nuclei. Interestingly, the same antibodies stain the majority of nonnucleolar nascent transcripts from the loops of lampbrush chromosomes in the newt, but the most intense staining is localized to the landmark giant loops. The L protein is the first protein of giant loops identified so far, and antibodies to it thus provide a useful tool with which to study these unique RNAs. In addition, isolation and sequencing of cDNA clones for the L protein from human cells predicts a glycine- and proline-rich protein of 60,187 D, which contains two 80 amino acid segments only distantly related to the RNP consensus sequence-type RNA- binding domain. The L protein, therefore, is a new type of hnRNP protein.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Hamilton, B. J., Wang, X.-W., Collins, J., Bloch, D., Bergeron, A., Henry, B., Terry, B. M., Zan, M., Mouland, A. J., Rigby, W. F. C. (2008). Separate cis-trans Pathways Post-transcriptionally Regulate Murine CD154 (CD40 Ligand) Expression: A NOVEL FUNCTION FOR CA REPEATS IN THE 3'-UNTRANSLATED REGION. J. Biol. Chem. 283: 25606-25616 [Abstract] [Full Text]
Hung, L.-H., Heiner, M., Hui, J., Schreiner, S., Benes, V., Bindereif, A. (2008). Diverse roles of hnRNP L in mammalian mRNA processing: A combined microarray and RNAi analysis. RNA 14: 284-296 [Abstract] [Full Text]
Cheli, Y., Kunicki, T. J. (2006). hnRNP L regulates differences in expression of mouse integrin {alpha}2beta1. Blood 107: 4391-4398 [Abstract] [Full Text]
Guang, S., Felthauser, A. M., Mertz, J. E. (2005). Binding of hnRNP L to the Pre-mRNA Processing Enhancer of the Herpes Simplex Virus Thymidine Kinase Gene Enhances both Polyadenylation and Nucleocytoplasmic Export of Intronless mRNAs. Mol. Cell. Biol. 25: 6303-6313 [Abstract] [Full Text]
Sallacz, N. B., Jantsch, M. F. (2005). Chromosomal Storage of the RNA-editing Enzyme ADAR1 in Xenopus Oocytes. Mol. Biol. Cell 16: 3377-3386 [Abstract] [Full Text]
Cazalla, D., Newton, K., Caceres, J. F. (2005). A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing. Mol. Cell. Biol. 25: 2969-2980 [Abstract] [Full Text]
Layalle, S., Coessens, E., Ghysen, A., Dambly-Chaudiere, C. (2005). Smooth, a hnRNP encoding gene, controls axonal navigation in Drosophila. GENES CELLS 10: 119-125 [Abstract] [Full Text]
Hall, M. P., Huang, S., Black, D. L. (2004). Differentiation-induced Colocalization of the KH-type Splicing Regulatory Protein with Polypyrimidine Tract Binding Protein and the c-src Pre-mRNA. Mol. Biol. Cell 15: 774-786 [Abstract] [Full Text]
HUI, J., REITHER, G., BINDEREIF, A. (2003). Novel functional role of CA repeats and hnRNP L in RNA stability. RNA 9: 931-936 [Abstract] [Full Text]
Honig, A., Auboeuf, D., Parker, M. M., O'Malley, B. W., Berget, S. M. (2002). Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box RNA Helicase p72. Mol. Cell. Biol. 22: 5698-5707 [Abstract] [Full Text]
Soderberg, M., Raffalli-Mathieu, F., Lang, M. A. (2002). Inflammation Modulates the Interaction of Heterogeneous Nuclear Ribonucleoprotein (hnRNP) I/Polypyrimidine Tract Binding Protein and hnRNP L with the 3'Untranslated Region of the Murine Inducible Nitric-Oxide Synthase mRNA. Mol. Pharmacol. 62: 423-431 [Abstract] [Full Text]
Handwerger, K. E., Wu, Z.'a., Murphy, C., Gall, J. G. (2002). Heat shock induces mini-Cajal bodies in the Xenopus germinal vesicle. J. Cell Sci. 115: 2011-2020 [Abstract] [Full Text]
Martens, J. H. A., Verlaan, M., Kalkhoven, E., Dorsman, J. C., Zantema, A. (2002). Scaffold/Matrix Attachment Region Elements Interact with a p300-Scaffold Attachment Factor A Complex and Are Bound by Acetylated Nucleosomes. Mol. Cell. Biol. 22: 2598-2606 [Abstract] [Full Text]
Kuninger, D. T., Izumi, T., Papaconstantinou, J., Mitra, S. (2002). Human AP-endonuclease 1 and hnRNP-L interact with a nCaRE-like repressor element in the AP-endonuclease 1 promoter. Nucleic Acids Res 30: 823-829 [Abstract] [Full Text]
Tian, H. (2002). Combinatorial Selection of RNA Ligands for Complex Cellular Targets : The RNA Liagands-Based Proteomics. Mol. Cell. Proteomics 1: 99-103 [Abstract] [Full Text]
Snee, M., Kidd, G. J., Munro, T. P., Smith, R. (2002). RNA trafficking and stabilization elements associate with multiple brain proteins. J. Cell Sci. 115: 4661-4669 [Abstract] [Full Text]
Mili, S., Shu, H. J., Zhao, Y., Pinol-Roma, S. (2001). Distinct RNP Complexes of Shuttling hnRNP Proteins with Pre-mRNA and mRNA: Candidate Intermediates in Formation and Export of mRNA. Mol. Cell. Biol. 21: 7307-7319 [Abstract] [Full Text]
Denegri, M., Chiodi, I., Corioni, M., Cobianchi, F., Riva, S., Biamonti, G. (2001). Stress-induced Nuclear Bodies Are Sites of Accumulation of Pre-mRNA Processing Factors. Mol. Biol. Cell 12: 3502-3514 [Abstract] [Full Text]
Ito, M., Shichijo, S., Tsuda, N., Ochi, M., Harashima, N., Saito, N., Itoh, K. (2001). Molecular Basis of T Cell-mediated Recognition of Pancreatic Cancer Cells. Cancer Res. 61: 2038-2046 [Abstract] [Full Text]
Vautier, D, Chesne, P, Cunha, C, Calado, A, Renard, J., Carmo-Fonseca, M (2001). Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos. J. Cell Sci. 114: 1521-1531 [Abstract]
Yamamoto, H., Tsukahara, K., Kanaoka, Y., Jinno, S., Okayama, H. (1999). Isolation of a Mammalian Homologue of a Fission Yeast Differentiation Regulator. Mol. Cell. Biol. 19: 3829-3841 [Abstract] [Full Text]
Shih, S.-C., Claffey, K. P. (1999). Regulation of Human Vascular Endothelial Growth Factor mRNA Stability in Hypoxia by Heterogeneous Nuclear Ribonucleoprotein L. J. Biol. Chem. 274: 1359-1365 [Abstract] [Full Text]
Hahm, B., Kim, Y. K., Kim, J. H., Kim, T. Y., Jang, S. K. (1998). Heterogeneous Nuclear Ribonucleoprotein L Interacts with the 3' Border of the Internal Ribosomal Entry Site of Hepatitis C Virus. J. Virol. 72: 8782-8788 [Abstract] [Full Text]
Schul, W., van Driel, R., de Jong, L. (1998). Coiled Bodies and U2 snRNA Genes Adjacent to Coiled Bodies Are Enriched in Factors Required for snRNA Transcription. Mol. Biol. Cell 9: 1025-1036 [Abstract] [Full Text]
Wang, J., Dong, Z., Bell, L. R. (1997). Sex-lethal Interactions with Protein and RNA. ROLES OF GLYCINE-RICH AND RNA BINDING DOMAINS. J. Biol. Chem. 272: 22227-22235 [Abstract] [Full Text]
Eckmann, C. R., Jantsch, M. F. (1997). Xlrbpa, a Double-stranded RNA-binding Protein Associated with Ribosomes and Heterogeneous Nuclear RNPs. J. Cell Biol. 138: 239-253 [Abstract] [Full Text]
Nishiyama, H., Itoh, K., Kaneko, Y., Kishishita, M., Yoshida, O., Fujita, J. (1997). A Glycine-rich RNA-binding Protein Mediating Cold-inducible Suppression of Mammalian Cell Growth. J. Cell Biol. 137: 899-908 [Abstract] [Full Text]
Mahe, D., Mahl, P., Gattoni, R., Fischer, N., Mattei, M.-G., Stevenin, J., Fuchs, J.-P. (1997). Cloning of Human 2H9 Heterogeneous Nuclear Ribonucleoproteins. RELATION WITH SPLICING AND EARLY HEAT SHOCK-INDUCED SPLICING ARREST. J. Biol. Chem. 272: 1827-1836 [Abstract] [Full Text]
Cotto, J, Fox, S, Morimoto, R (1997). HSF1 granules: a novel stress-induced nuclear compartment of human cells. J. Cell Sci. 110: 2925-2934 [Abstract]
Grande, M., van der Kraan, I, de Jong, L, van Driel, R (1997). Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II. J. Cell Sci. 110: 1781-1791 [Abstract]
Inagaki, H., Matsushima, Y., Nakamura, K., Ohshima, M., Kadowaki, T., Kitagawa, Y. (1996). A Large DNA-binding Nuclear Protein with RNA Recognition Motif and Serine/Arginine-rich Domain. J. Biol. Chem. 271: 12525-12531 [Abstract] [Full Text]
Liu, X, Mertz, J E (1995). HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.. Genes Dev. 9: 1766-1780 [Abstract]
Solovei, I, Macgregor, H, Gaginskaya, E (1995). Single stranded nucleic acid binding structures on chicken lampbrush chromosomes. J. Cell Sci. 108: 1391-1396 [Abstract]
Wang, J, Bell, L R (1994). The Sex-lethal amino terminus mediates cooperative interactions in RNA binding and is essential for splicing regulation.. Genes Dev. 8: 2072-2085 [Abstract]
Patton, J G, Porro, E B, Galceran, J, Tempst, P, Nadal-Ginard, B (1993). Cloning and characterization of PSF, a novel pre-mRNA splicing factor.. Genes Dev. 7: 393-406 [Abstract]
Gil, A, Sharp, P A, Jamison, S F, Garcia-Blanco, M A (1991). Characterization of cDNAs encoding the polypyrimidine tract-binding protein.. Genes Dev. 5: 1224-1236 [Abstract]
Patton, J G, Mayer, S A, Tempst, P, Nadal-Ginard, B (1991). Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.. Genes Dev. 5: 1237-1251 [Abstract]
Takagaki, Y, Manley, J L, MacDonald, C C, Wilusz, J, Shenk, T (1990). A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.. Genes Dev. 4: 2112-2120 [Abstract]
Stoss, O., Olbrich, M., Hartmann, A. M., Konig, H., Memmott, J., Andreadis, A., Stamm, S. (2001). The STAR/GSG Family Protein rSLM-2 Regulates the Selection of Alternative Splice Sites. J. Biol. Chem. 276: 8665-8673 [Abstract] [Full Text]