The Journal of Cell Biology, Vol 109, 2869-2878, Copyright © 1989 by The Rockefeller University Press

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Mass isolation of calf thymus centrosomes: identification of a specific configuration

S Komesli, F Tournier, M Paintrand, RL Margolis, D Job and M Bornens
Laboratoire Biochimie des Regulations Cellulaires Endocrines, Institut National de la Sante et de la Recherche Medicale U244, Grenoble, France.

Centrosomes from calf thymocytes were isolated using a simple preparative procedure that provides large yields of free organelles. A comparative study with centrosomes isolated from human cultured lymphoblasts has led to the discovery of important differences in the structure of the two isolates and in their capacity to nucleate microtubules from purified tubulin. The possibility that the centrosomal structure depends upon the growth state of cells is discussed.
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