Structural changes and lateral redistribution of photosystem II during donor side photoinhibition of thylakoids

doi:10.1083/jcb.119.2.325

This Article

	Full Text (PDF, 2692K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Barbato, R.
	Articles by Giacometti, G. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Barbato, R.
	Articles by Giacometti, G. M.


Social Bookmarking

	What's this?

ARTICLES

Structural changes and lateral redistribution of photosystem II during donor side photoinhibition of thylakoids

R Barbato, G Friso, F Rigoni, F Dalla Vecchia and GM Giacometti
Dipartimento di Biologia, Universita di Padova, Italy.

The structural and topological stability of thylakoid components under photoinhibitory conditions (4,500 microE.m-2.s-1 white light) was studied on Mn depleted thylakoids isolated from spinach leaves. After various exposures to photoinhibitory light, the chlorophyll-protein complexes of both photosystems I and II were separated by sucrose gradient centrifugation and analysed by Western blotting, using a set of polyclonals raised against various apoproteins of the photosynthetic apparatus. A series of events occurring during donor side photoinhibition are described for photosystem II, including: (a) lowering of the oligomerization state of the photosystem II core; (b) cleavage of 32-kD protein D1 at specific sites; (c) dissociation of chlorophyll-protein CP43 from the photosystem II core; and (d) migration of damaged photosystem II components from the grana to the stroma lamellae. A tentative scheme for the succession of these events is illustrated. Some effects of photoinhibition on photosystem I are also reported involving dissociation of antenna chlorophyll-proteins LHCI from the photosystem I reaction center.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Pagliano, C., La Rocca, N., Andreucci, F., Deak, Z., Vass, I., Rascio, N., Barbato, R. (2008). The extreme halophyte Salicornia veneta is depleted of the extrinsic PsbQ and PsbP proteins of the oxygen-evolving complex without loss of functional activity. ANN BOT (LOND) 0: mcn234v1-11 [Abstract] [Full Text]
Balogi, Z., Cheregi, O., Giese, K. C., Juhasz, K., Vierling, E., Vass, I., Vigh, L., Horvath, I. (2008). A Mutant Small Heat Shock Protein with Increased Thylakoid Association Provides an Elevated Resistance Against UV-B Damage in Synechocystis 6803. J. Biol. Chem. 283: 22983-22991 [Abstract] [Full Text]
Andreucci, F., Barbato, R., Picollo, C., Segalla, A. (2005). Isolation of phosphorylated and dephosphorylated forms of the CP43 internal antenna of photosystem II in Hordeum vulgare L.. J Exp Bot 56: 1239-1244 [Abstract] [Full Text]
Bergo, E., Segalla, A., Giacometti, G. M., Tarantino, D., Soave, C., Andreucci, F., Barbato, R. (2003). Role of visible light in the recovery of photosystem II structure and function from ultraviolet-B stress in higher plants. J Exp Bot 54: 1665-1673 [Abstract] [Full Text]
Vecchia, F. D., Barbato, R., La Rocca, N., Moro, I., Rascio, N. (2001). Responses to bleaching herbicides by leaf chloroplasts of maize plants grown at different temperatures. J Exp Bot 52: 811-820 [Abstract] [Full Text]
Yamamoto, Y. (2001). Quality Control of Photosystem II. Plant Cell Physiol 42: 121-128 [Abstract] [Full Text]
Zhang, L., Paakkarinen, V., van Wijk, K. J., Aro, E.-M. (2000). Biogenesis of the Chloroplast-Encoded D1 Protein: Regulation of Translation Elongation, Insertion, and Assembly into Photosystem II. Plant Cell 12: 1769-1782 [Abstract] [Full Text]
Barbato, R., Bergo, E., Szabo, I., Dalla Vecchia, F., Giacometti, G. M. (2000). Ultraviolet B Exposure of Whole Leaves of Barley Affects Structure and Functional Organization of Photosystem II. J. Biol. Chem. 275: 10976-10982 [Abstract] [Full Text]
Kruse, O., Hankamer, B., Konczak, C., Gerle, C., Morris, E., Radunz, A., Schmid, G. H., Barber, J. (2000). Phosphatidylglycerol Is Involved in the Dimerization of Photosystem II. J. Biol. Chem. 275: 6509-6514 [Abstract] [Full Text]
Spetea, C., Hundal, T., Lohmann, F., Andersson, B. (1999). GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. Proc. Natl. Acad. Sci. USA 96: 6547-6552 [Abstract] [Full Text]
Bianchetti, M., Zheleva, D., Deak, Z., Zharmuhamedov, S., Klimov, V., Nugent, J., Vass, I., Barber, J. (1998). Comparison of the Functional Properties of the Monomeric and Dimeric Forms of the Isolated CP47-Reaction Center Complex. J. Biol. Chem. 273: 16128-16133 [Abstract] [Full Text]
van Wijk, K. J., Andersson, B., Aro, E.-M. (1996). Kinetic Resolution of the Incorporation of the D1 Protein into Photosystem II and Localization of Assembly Intermediates in Thylakoid Membranes of Spinach Chloroplasts. J. Biol. Chem. 271: 9627-9636 [Abstract] [Full Text]
van Wijk, K. J., Bingsmark, S., Aro, E.-M., Andersson, B. (1995). In Vitro Synthesis and Assembly of Photosystem II Core Proteins. J. Biol. Chem. 270: 25685-25695 [Abstract] [Full Text]
Bergantino, E., Dainese, P., Cerovic, Z., Sechi, S., Bassi, R. (1995). A Post-translational Modification of the Photosystem II Subunit CP29 Protects Maize from Cold Stress. J. Biol. Chem. 270: 8474-8481 [Abstract] [Full Text]
Szabo, I., Seraglia, R., Rigoni, F., Traldi, P., Giacometti, G. M. (2001). Determination of Photosystem II Subunits by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry. J. Biol. Chem. 276: 13784-13790 [Abstract] [Full Text]