Identification and characterization of a sphere organelle protein

doi:10.1083/jcb.122.4.767

This Article

	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Tuma, R. S.
	Articles by Roth, M. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Tuma, R. S.
	Articles by Roth, M. B.


Social Bookmarking

	What's this?

ARTICLES

Identification and characterization of a sphere organelle protein

RS Tuma, JA Stolk and MB Roth
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

Sphere organelles are nuclear structures in amphibian oocytes that are easily visible by light microscopy. These structures are up to 10 microns in diameter and have been described morphologically for decades, yet their function remains obscure. The present study defines a protein component of the sphere organelle, named SPH-1, which is recognized by a mAb raised against purified Xenopus laevis oocyte nucleoplasm. SPH-1 is an 80-kD protein which is localized specifically to spheres and is undetectable elsewhere on lampbrush chromosomes or in nucleoli. We show using confocal microscopy that SPH-1 is localized to the cortex of sphere organelles. Furthermore, we have isolated a cDNA that can encode SPH-1. When epitope-tagged forms of SPH-1 are expressed in X. laevis oocytes the protein specifically localizes to spheres, demonstrating that the cloned cDNA encodes the sphere antigen. Comparison of the predicted amino acid sequence with sequence databases shows SPH-1 is related to p80-coilin, a protein associated with coiled bodies; coiled bodies are nuclear structures found in plant and animal cells. The sphere-specific mAb stains X. laevis tissue culture cells in a punctate nuclear pattern, showing that spheres or sphere antigens are present in somatic cells as well as germ cells and suggesting a general and essential function for spheres in all nuclei.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Morency, E., Sabra, M., Catez, F., Texier, P., Lomonte, P. (2007). A novel cell response triggered by interphase centromere structural instability. J. Cell Biol. 177: 757-768 [Abstract] [Full Text]
Collier, S., Pendle, A., Boudonck, K., van Rij, T., Dolan, L., Shaw, P. (2006). A Distant Coilin Homologue Is Required for the Formation of Cajal Bodies in Arabidopsis. Mol. Biol. Cell 17: 2942-2951 [Abstract] [Full Text]
Liu, J.-L., Murphy, C., Buszczak, M., Clatterbuck, S., Goodman, R., Gall, J. G. (2006). The Drosophila melanogaster Cajal body.. J. Cell Biol. 172: 875-884 [Abstract] [Full Text]
Ling, Y., Smith, A. J., Morgan, G. T. (2006). A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS.. Nucleic Acids Res 34: 2219-2229 [Abstract] [Full Text]
Masuyama, K., Taniguchi, I., Kataoka, N., Ohno, M. (2004). SR proteins preferentially associate with mRNAs in the nucleus and facilitate their export to the cytoplasm. GENES CELLS 9: 959-965 [Abstract] [Full Text]
Smith, A. J., Ling, Y., Morgan, G. T. (2003). Subnuclear Localization and Cajal Body Targeting of Transcription Elongation Factor TFIIS in Amphibian Oocytes. Mol. Biol. Cell 14: 1255-1267 [Abstract] [Full Text]
Stanek, D., Rader, S. D., Klingauf, M., Neugebauer, K. M. (2003). Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies. J. Cell Biol. 160: 505-516 [Abstract] [Full Text]
Handwerger, K. E., Wu, Z.'a., Murphy, C., Gall, J. G. (2002). Heat shock induces mini-Cajal bodies in the Xenopus germinal vesicle. J. Cell Sci. 115: 2011-2020 [Abstract] [Full Text]
Hofmann, I., Schnolzer, M., Kaufmann, I., Franke, W. W. (2002). Symplekin, a Constitutive Protein of Karyo- and Cytoplasmic Particles Involved in mRNA Biogenesis in Xenopus laevis Oocytes. Mol. Biol. Cell 13: 1665-1676 [Abstract] [Full Text]
Kneissel, S., Franke, W. W., Gall, J. G., Heid, H., Reidenbach, S., Schnolzer, M., Spring, H., Zentgraf, H., Schmidt-Zachmann, M. S. (2001). A Novel Karyoskeletal Protein: Characterization of Protein NO145, the Major Component of Nucleolar Cortical Skeleton in Xenopus Oocytes. Mol. Biol. Cell 12: 3904-3918 [Abstract] [Full Text]
Tucker, K. E., Berciano, M. T., Jacobs, E. Y., LePage, D. F., Shpargel, K. B., Rossire, J. J., Chan, E. K.L., Lafarga, M., Conlon, R. A., Matera, A. G. (2001). Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product. J. Cell Biol. 154: 293-308 [Abstract] [Full Text]
Speckmann, W., Narayanan, A., Terns, R., Terns, M. P. (1999). Nuclear Retention Elements of U3 Small Nucleolar RNA. Mol. Cell. Biol. 19: 8412-8421 [Abstract] [Full Text]
Gall, J. G., Bellini, M., Wu, Z.'a., Murphy, C. (1999). Assembly of the Nuclear Transcription and Processing Machinery: Cajal Bodies (Coiled Bodies) and Transcriptosomes. Mol. Biol. Cell 10: 4385-4402 [Abstract] [Full Text]
Bellini, M., Gall, J. G. (1999). Coilin Shuttles between the Nucleus and Cytoplasm In Xenopus Oocytes. Mol. Biol. Cell 10: 3425-3434 [Abstract] [Full Text]
Narayanan, A., Speckmann, W., Terns, R., Terns, M. P. (1999). Role of the Box C/D Motif in Localization of Small Nucleolar RNAs to Coiled Bodies and Nucleoli. Mol. Biol. Cell 10: 2131-2147 [Abstract] [Full Text]
Abbott, J., Marzluff, W. F., Gall, J. G. (1999). The Stem-Loop Binding Protein (SLBP1) Is Present in Coiled Bodies of the Xenopus Germinal Vesicle. Mol. Biol. Cell 10: 487-499 [Abstract] [Full Text]
Bellini, M., Gall, J. G. (1998). Coilin Can Form a Complex with the U7 Small Nuclear Ribonucleoprotein. Mol. Biol. Cell 9: 2987-3001 [Abstract] [Full Text]
Almeida, F., Saffrich, R., Ansorge, W., Carmo-Fonseca, M. (1998). Microinjection of Anti-coilin Antibodies Affects the Structure of Coiled Bodies. J. Cell Biol. 142: 899-912 [Abstract] [Full Text]
Lamond, A. I., Earnshaw, W. C. (1998). Structure and Function in the Nucleus. Science 280: 547-553 [Abstract] [Full Text]
Neugebauer, K M, Roth, M B (1997). Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription.. Genes Dev. 11: 1148-1159 [Abstract]
Bell, P, Scheer, U (1997). Prenucleolar bodies contain coilin and are assembled in Xenopus egg extract depleted of specific nucleolar proteins and U3 RNA. J. Cell Sci. 110: 43-54 [Abstract]
Ferreira, J, Carmo-Fonseca, M (1995). The biogenesis of the coiled body during early mouse development. Development 121: 601-612 [Abstract]
Ochs, R., Stein, T., Tan, E. (1994). Coiled bodies in the nucleolus of breast cancer cells. J. Cell Sci. 107: 385-399 [Abstract]
Muller, B., Link, J., Smythe, C. (2000). Assembly of U7 Small Nuclear Ribonucleoprotein Particle and Histone RNA 3' Processing in Xenopus Egg Extracts. J. Biol. Chem. 275: 24284-24293 [Abstract] [Full Text]