Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans- activation

doi:10.1083/jcb.123.6.1309

This Article

	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ruhl, M.
	Articles by Bevec, D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ruhl, M.
	Articles by Bevec, D.


Social Bookmarking

	What's this?

ARTICLES

Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans- activation

M Ruhl, M Himmelspach, GM Bahr, F Hammerschmid, H Jaksche, B Wolff, H Aschauer, GK Farrington, H Probst and D Bevec
SANDOZ Research Institute, Vienna, Austria.

Expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA. Furthermore, the interaction of the Rev activation domain with a cellular cofactor is essential for Rev function in vivo. Using cross-linking experiments and Biospecific Interaction Analysis (BIA) we identify eukaryotic initiation factor 5A (eIF-5A) as a cellular factor binding specifically to the HIV-1 Rev activation domain. Indirect immunofluorescence studies demonstrate that a significant fraction of eIF-5A localizes to the nucleus. We also provide evidence that Rev transactivation is functionally mediated by eIF-5A in Xenopus oocytes. Furthermore, we are able to block Rev function in mammalian cells by antisense inhibition of eIF-5A gene expression. Thus, regulation of HIV-1 gene expression by Rev involves the targeting of RRE-containing RNA to components of the cellular translation initiation complex.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Edgcomb, S. P., Aschrafi, A., Kompfner, E., Williamson, J. R., Gerace, L., Hennig, M. (2008). Protein structure and oligomerization are important for the formation of export-competent HIV-1 Rev-RRE complexes. Protein Sci. 17: 420-430 [Abstract] [Full Text]
Kang, K. R., Kim, Y. S., Wolff, E. C., Park, M. H. (2007). Specificity of the Deoxyhypusine Hydroxylase-Eukaryotic Translation Initiation Factor (eIF5A) Interaction: IDENTIFICATION OF AMINO ACID RESIDUES OF THE ENZYME REQUIRED FOR BINDING OF ITS SUBSTRATE, DEOXYHYPUSINE-CONTAINING eIF5A. J. Biol. Chem. 282: 8300-8308 [Abstract] [Full Text]
Huang, Y., Higginson, D. S., Hester, L., Park, M. H., Snyder, S. H. (2007). Neuronal growth and survival mediated by eIF5A, a polyamine-modified translation initiation factor. Proc. Natl. Acad. Sci. USA 104: 4194-4199 [Abstract] [Full Text]
Fries, B., Heukeshoven, J., Hauber, I., Gruttner, C., Stocking, C., Kehlenbach, R. H., Hauber, J., Chemnitz, J. (2007). Analysis of Nucleocytoplasmic Trafficking of the HuR Ligand APRIL and Its Influence on CD83 Expression. J. Biol. Chem. 282: 4504-4515 [Abstract] [Full Text]
Schrader, R., Young, C., Kozian, D., Hoffmann, R., Lottspeich, F. (2006). Temperature-sensitive eIF5A Mutant Accumulates Transcripts Targeted to the Nonsense-mediated Decay Pathway. J. Biol. Chem. 281: 35336-35346 [Abstract] [Full Text]
Kim, Y. S., Kang, K. R., Wolff, E. C., Bell, J. K., McPhie, P., Park, M. H. (2006). Deoxyhypusine Hydroxylase Is an Fe(II)-dependent, Heat-repeat Enzyme: IDENTIFICATION OF AMINO ACID RESIDUES CRITICAL FOR Fe(II) BINDING AND CATALYSIS. J. Biol. Chem. 281: 13217-13225 [Abstract] [Full Text]
Parker, R., Flowers, T. J., Moore, A. L., Harpham, N. V. J. (2006). An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina. J Exp Bot 57: 1109-1118 [Abstract] [Full Text]
Park, M. H. (2006). The Post-Translational Synthesis of a Polyamine-Derived Amino Acid, Hypusine, in the Eukaryotic Translation Initiation Factor 5A (eIF5A). J Biochem 139: 161-169 [Abstract] [Full Text]
Zanelli, C. F., Valentini, S. R. (2005). Pkc1 Acts Through Zds1 and Gic1 to Suppress Growth and Cell Polarity Defects of a Yeast eIF5A Mutant. Genetics 171: 1571-1581 [Abstract] [Full Text]
Bennett, E. M., Lever, A. M. L., Allen, J. F. (2004). Human Immunodeficiency Virus Type 2 Gag Interacts Specifically with PRP4, a Serine-Threonine Kinase, and Inhibits Phosphorylation of Splicing Factor SF2. J. Virol. 78: 11303-11312 [Abstract] [Full Text]
Sommer, M.-N., Bevec, D., Klebl, B., Flicke, B., Holscher, K., Freudenreich, T., Hauber, I., Hauber, J., Mett, H. (2004). Screening Assay for the Identification of Deoxyhypusine Synthase Inhibitors. J Biomol Screen 9: 434-438 [Abstract]
Li, J., Liu, Y., Kim, B. O., He, J. J. (2002). Direct Participation of Sam68, the 68-Kilodalton Src-Associated Protein in Mitosis, in the CRM1-Mediated Rev Nuclear Export Pathway. J. Virol. 76: 8374-8382 [Abstract] [Full Text]
Li, J., Liu, Y., Park, I.-W., He, J. J. (2002). Expression of Exogenous Sam68, the 68-Kilodalton Src-Associated Protein in Mitosis, Is Able To Alleviate Impaired Rev Function in Astrocytes. J. Virol. 76: 4526-4535 [Abstract] [Full Text]
Yi, R., Bogerd, H. P., Cullen, B. R. (2002). Recruitment of the Crm1 Nuclear Export Factor Is Sufficient To Induce Cytoplasmic Expression of Incompletely Spliced Human Immunodeficiency Virus mRNAs. J. Virol. 76: 2036-2042 [Abstract] [Full Text]
Reddy, T. R., Suhasini, M., Xu, W., Yeh, L.-y., Yang, J.-P., Wu, J., Artzt, K., Wong-Staal, F. (2002). A Role for KH Domain Proteins (Sam68-like Mammalian Proteins and Quaking Proteins) in the Post-transcriptional Regulation of HIV Replication. J. Biol. Chem. 277: 5778-5784 [Abstract] [Full Text]
Valentini, S. R., Casolari, J. M., Oliveira, C. C., Silver, P. A., McBride, A. E. (2002). Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-Binding Protein and Protein Kinase C Signaling. Genetics 160: 393-405 [Abstract] [Full Text]
Nam, Y.-S., Petrovic, A., Jeong, K.-S., Venkatesan, S. (2001). Exchange of the Basic Domain of Human Immunodeficiency Virus Type 1 Rev for a Polyarginine Stretch Expands the RNA Binding Specificity, and a Minimal Arginine Cluster Is Required for Optimal RRE RNA Binding Affinity, Nuclear Accumulation, and trans-Activation. J. Virol. 75: 2957-2971 [Abstract] [Full Text]
Hofmann, W., Reichart, B., Ewald, A., Muller, E., Schmitt, I., Stauber, R. H., Lottspeich, F., Jockusch, B. M., Scheer, U., Hauber, J., Dabauvalle, M.-C. (2001). Cofactor Requirements for Nuclear Export of Rev Response Element (RRE)- and Constitutive Transport Element (CTE)-containing Retroviral RNAs: An Unexpected Role for Actin. J. Cell Biol. 152: 895-910 [Abstract] [Full Text]
D'Agostino, D. M., Ferro, T., Zotti, L., Meggio, F., Pinna, L. A., Chieco-Bianchi, L., Ciminale, V. (2000). Identification of a Domain in Human Immunodeficiency Virus Type 1 Rev That Is Required for Functional Activity and Modulates Association with Subnuclear Compartments Containing Splicing Factor SC35. J. Virol. 74: 11899-11910 [Abstract] [Full Text]
Kruse, M., Rosorius, O., Krätzer, F., Stelz, G., Kuhnt, C., Schuler, G., Hauber, J., Steinkasserer, A. (2000). Mature Dendritic Cells Infected with Herpes Simplex Virus Type 1 Exhibit Inhibited T-Cell Stimulatory Capacity. J. Virol. 74: 7127-7136 [Abstract] [Full Text]
Kruse, M., Rosorius, O., Kratzer, F., Bevec, D., Kuhnt, C., Steinkasserer, A., Schuler, G., Hauber, J. (2000). Inhibition of CD83 Cell Surface Expression during Dendritic Cell Maturation by Interference with Nuclear Export of CD83 mRNA. J. Exp. Med. 191: 1581-1590 [Abstract] [Full Text]
Rosorius, O., Fries, B., Stauber, R. H., Hirschmann, N., Bevec, D., Hauber, J. (2000). Human Ribosomal Protein L5 Contains Defined Nuclear Localization and Export Signals. J. Biol. Chem. 275: 12061-12068 [Abstract] [Full Text]
Ober, D., Hartmann, T. (1999). Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase. Proc. Natl. Acad. Sci. USA 96: 14777-14782 [Abstract] [Full Text]
Morehouse, H., Buratowski, R. M., Silver, P. A., Buratowski, S. (1999). The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc. Natl. Acad. Sci. USA 96: 12542-12547 [Abstract] [Full Text]
Szilvay, A. M., Bøe, S.-O., Kalland, K.-H. (1999). Co-expression of a trans-dominant negative mutant of the human immunodeficiency virus type 1 (HIV-1) Rev protein affects the Rev-dependent splicing pattern and expression of HIV-1 RNAs. J. Gen. Virol. 80: 1965-1974 [Abstract] [Full Text]
Hamm, T. E., Rekosh, D., Hammarskjöld, M.-L. (1999). Selection and Characterization of Human Immunodeficiency Virus Type 1�Mutants That Are Resistant to Inhibition by the Transdominant Negative RevM10 Protein. J. Virol. 73: 5741-5747 [Abstract] [Full Text]
Van Ryk, D. I., Venkatesan, S. (1999). Real-time Kinetics of HIV-1 Rev-Rev Response Element Interactions. DEFINITION OF MINIMAL BINDING SITES ON RNA AND PROTEIN AND STOICHIOMETRIC ANALYSIS. J. Biol. Chem. 274: 17452-17463 [Abstract] [Full Text]
Elfgang, C., Rosorius, O., Hofer, L., Jaksche, H., Hauber, J., Bevec, D. (1999). Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals. Proc. Natl. Acad. Sci. USA 96: 6229-6234 [Abstract] [Full Text]
Symensma, T. L., Baskerville, S., Yan, A., Ellington, A. D. (1999). Polyvalent Rev Decoys Act as Artificial Rev-Responsive Elements. J. Virol. 73: 4341-4349 [Abstract] [Full Text]
Wood, L. C., Ashby, M. N., Grunfeld, C., Feingold, K. R. (1999). Cloning of Murine Translation Initiation Factor 6�and Functional Analysis of the Homologous Sequence YPR016c in Saccharomyces cerevisiae. J. Biol. Chem. 274: 11653-11659 [Abstract] [Full Text]
Li, J., Tang, H., Mullen, T.-M., Westberg, C., Reddy, T. R., Rose, D. W., Wong-Staal, F. (1999). A role for RNA helicase A in post-transcriptional regulation of HIV type 1. Proc. Natl. Acad. Sci. USA 96: 709-714 [Abstract] [Full Text]
Rosorius, O, Reichart, B, Kratzer, F, Heger, P, Dabauvalle, M., Hauber, J (1999). Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1. J. Cell Sci. 112: 2369-2380 [Abstract]
Seguin, B., Staffa, A., Cochrane, A. (1998). Control of Human Immunodeficiency Virus Type 1�RNA Metabolism: Role of Splice Sites and Intron Sequences in Unspliced Viral RNA Subcellular Distribution. J. Virol. 72: 9503-9513 [Abstract] [Full Text]
Heger, P., Rosorius, O., Koch, C., Casari, G., Grassmann, R., Hauber, J. (1998). Multimer Formation Is Not Essential for Nuclear Export of Human T-Cell Leukemia Virus Type 1�Rex trans-Activator Protein. J. Virol. 72: 8659-8668 [Abstract] [Full Text]
Stutz, F., Rosbash, M. (1998). Nuclear RNA export. Genes Dev. 12: 3303-3319 [Full Text]
Kim, K. K., Hung, L.-W., Yokota, H., Kim, R., Kim, S.-H. (1998). Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8�A�resolution. Proc. Natl. Acad. Sci. USA 95: 10419-10424 [Abstract] [Full Text]
Kehlenbach, R. H., Dickmanns, A., Gerace, L. (1998). Nucleocytoplasmic Shuttling Factors Including Ran and CRM1 Mediate Nuclear Export of NFAT In Vitro. J. Cell Biol. 141: 863-874 [Abstract] [Full Text]
Thomas, S. L., Oft, M., Jaksche, H., Casari, G., Heger, P., Dobrovnik, M., Bevec, D., Hauber, J. (1998). Functional Analysis of the Human Immunodeficiency Virus Type 1�Rev Protein Oligomerization Interface. J. Virol. 72: 2935-2944 [Abstract] [Full Text]
Schatz, O., Oft, M., Dascher, C., Schebesta, M., Rosorius, O., Jaksche, H., Dobrovnik, M., Bevec, D., Hauber, J. (1998). Interaction of the HIV-1 Rev cofactor eukaryotic initiation factor 5A with ribosomal�protein�L5. Proc. Natl. Acad. Sci. USA 95: 1607-1612 [Abstract] [Full Text]
Ranga, U., Woffendin, C., Verma, S., Xu, L., June, C. H., Bishop, D. K., Nabel, G. J. (1998). Enhanced T cell engraftment after retroviral delivery of an antiviral gene in HIV-infected�individuals. Proc. Natl. Acad. Sci. USA 95: 1201-1206 [Abstract] [Full Text]
Park, M. H., Joe, Y. A., Kang, K. R. (1998). Deoxyhypusine Synthase Activity Is Essential for Cell Viability in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 273: 1677-1683 [Abstract] [Full Text]
Bunnell, B. A., Morgan, R. A. (1998). Gene Therapy for Infectious Diseases. Clin. Microbiol. Rev. 11: 42-56 [Abstract] [Full Text]
Powell, D.�M., Amaral, M.�C., Wu, J.�Y., Maniatis, T., Greene, W.�C. (1997). HIV Rev-dependent binding of SF2/ASF to the Rev response element: Possible role in Rev-mediated inhibition of HIV RNA�splicing. Proc. Natl. Acad. Sci. USA 94: 973-978 [Abstract] [Full Text]
Xu, A., Chen, K. Y. (2001). Hypusine Is Required for a Sequence-specific Interaction of Eukaryotic Initiation Factor 5A with Postsystematic Evolution of Ligands by Exponential Enrichment RNA. J. Biol. Chem. 276: 2555-2561 [Abstract] [Full Text]