Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts

doi:10.1083/jcb.123.6.1321

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Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts

Y Kubota and H Takisawa
Department of Biology, Faculty of Science, Osaka University, Japan.

Xenopus egg extracts prepared before and after egg activation retain M- and S-phase specific activity, respectively. Staurosporine, a potent inhibitor of protein kinase, converted M-phase extracts into interphase- like extracts that were capable of forming nuclei upon the addition of sperm DNA. The nuclei formed in the staurosporine treated M-phase extract were incapable of replicating DNA, and they were unable to initiate replication upon the addition of S-phase extracts. Furthermore, replication was inhibited when the staurosporine-treated M- phase extract was added in excess to the staurosporine-treated S-phase extract before the addition of DNA. The membrane-depleted S-phase extract supported neither nuclear formation nor replication; however, preincubation of sperm DNA with these extracts allowed them to form replication-competent nuclei upon the addition of excess staurosporine- treated M-phase extract. These results demonstrate that positive factors in the S-phase extracts determined the initiation of DNA replication before nuclear formation, although these factors were unable to initiate replication after nuclear formation.
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Hua, X. H., Yan, H., Newport, J. (1997). A Role for Cdk2 Kinase in Negatively Regulating DNA Replication during S Phase of the Cell Cycle. J. Cell Biol. 137: 183-192 [Abstract] [Full Text]
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Strausfeld, U., Howell, M, Descombes, P, Chevalier, S, Rempel, R., Adamczewski, J, Maller, J., Hunt, T, Blow, J. (1996). Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts. J. Cell Sci. 109: 1555-1563 [Abstract]
Sanchez, J. A., Wonsey, D. R., Harris, L., Morales, J., Wangh, L. J. (1995). Efficient Plasmid DNA Replication in Xenopus Egg Extracts Does Not Depend on Prior Chromatin Assembly. J. Biol. Chem. 270: 29676-29681 [Abstract] [Full Text]