Beta 2 integrin engagement triggers actin polymerization and phosphatidylinositol trisphosphate formation in non-adherent human neutrophils

doi:10.1083/jcb.123.6.1597

This Article

	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lofgren, R.
	Articles by Andersson, T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lofgren, R.
	Articles by Andersson, T.


Social Bookmarking

	What's this?

ARTICLES

Beta 2 integrin engagement triggers actin polymerization and phosphatidylinositol trisphosphate formation in non-adherent human neutrophils

R Lofgren, J Ng-Sikorski, A Sjolander and T Andersson
Department of Cell Biology, University of Linkoping, Sweden.

Beta 2 integrins are involved in the adhesion of leukocytes to other cells and surfaces. Although adhesion is required for cell locomotion, little is known regarding the way beta 2 integrin-receptors affect the actin network in leukocytes. In the present study filamentous actin (F- actin) levels in non-adherent human neutrophils have been measured by phalloidin staining after antibody cross-linking of beta 2 integrins. Antibody engagement of beta 2 integrins resulted in a rapid and sustained (146 and 131% after 30 and 300 s, respectively) increase in the neutrophil F-actin content. This is in contrast to stimulation with N-formyl-l-methionyl-l-leucyl-l-phenylalanine (fMLP), which causes a prompt and pronounced but rapidly declining rise in F-actin (214 and 127% after 15 and 300 s, respectively). Priming neutrophils with 1 nM PMA, a low concentration that did not influence the F-actin content per se, increased the magnitude of the beta 2 integrin-induced response but had no effect on the kinetics (199% after 30 s and 169% after 300 s). Removal of extracellular Ca2+ only marginally affected the beta 2 integrin-induced F-actin response for cells that were pretreated with PMA whereas the response for nonprimed cells was reduced by half. This suggests that even though extracellular Ca2+ has a modulatory effect it is not an absolute requirement for beta 2 integrin-induced actin polymerization. beta 2 integrin engagement did not affect the resting cellular level of cAMP arguing against a role of cAMP in beta 2 integrin-induced actin assembly.(ABSTRACT TRUNCATED AT 250 WORDS)
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Lorenowicz, M. J., Fernandez-Borja, M., Hordijk, P. L. (2007). cAMP Signaling in Leukocyte Transendothelial Migration. Arterioscler. Thromb. Vasc. Bio. 27: 1014-1022 [Abstract] [Full Text]
Jenei, V., Deevi, R. K., Adams, C. A., Axelsson, L., Hirst, D. G., Andersson, T., Dib, K. (2006). Nitric Oxide Produced in Response to Engagement of beta2 Integrins on Human Neutrophils Activates the Monomeric GTPases Rap1 and Rap2 and Promotes Adhesion. J. Biol. Chem. 281: 35008-35020 [Abstract] [Full Text]
VanCompernolle, S. E., Clark, K. L., Rummel, K. A., Todd, S. C. (2003). Expression and Function of Formyl Peptide Receptors on Human Fibroblast Cells. J. Immunol. 171: 2050-2056 [Abstract] [Full Text]
Dib, K., Melander, F., Axelsson, L., Dagher, M.-C., Aspenstrom, P., Andersson, T. (2003). Down-regulation of Rac Activity during {beta}2 Integrin-mediated Adhesion of Human Neutrophils. J. Biol. Chem. 278: 24181-24188 [Abstract] [Full Text]
Silliman, C. C., Elzi, D. J., Ambruso, D. R., Musters, R. J., Hamiel, C., Harbeck, R. J., Paterson, A. J., Bjornsen, A. J., Wyman, T. H., Kelher, M., England, K. M., McLaughlin-Malaxecheberria, N., Barnett, C. C., Aiboshi, J., Bannerjee, A. (2003). Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium. J. Leukoc. Biol. 73: 511-524 [Abstract] [Full Text]
Wyman, T. H., Bjornsen, A. J., Elzi, D. J., Smith, C. W., England, K. M., Kelher, M., Silliman, C. C. (2002). A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine release. Am. J. Physiol. Cell Physiol. 283: C1592-C1603 [Abstract] [Full Text]
Dib, K., Melander, F., Andersson, T. (2001). Role of p190RhoGAP in {{beta}}2 Integrin Regulation of RhoA in Human Neutrophils. J. Immunol. 166: 6311-6322 [Abstract] [Full Text]
Takami, M., Herrera, R., Petruzzelli, L. (2001). Mac-1-dependent tyrosine phosphorylation during neutrophil adhesion. Am. J. Physiol. Cell Physiol. 280: C1045-C1056 [Abstract] [Full Text]
Lawrence, D. W., Pryzwansky, K. B. (2001). The Vasodilator-Stimulated Phosphoprotein Is Regulated by Cyclic GMP-Dependent Protein Kinase During Neutrophil Spreading. J. Immunol. 166: 5550-5556 [Abstract] [Full Text]
Cox, D., Dale, B. M., Kashiwada, M., Helgason, C. D., Greenberg, S. (2000). A Regulatory Role for Src Homology 2 Domain-containing Inositol 5'-Phosphatase (SHIP) in Phagocytosis Mediated by Fc{{gamma}} Receptors and Complement Receptor 3 ({{alpha}}M{beta}2; CD11b/CD18). J. Exp. Med. 193: 61-72 [Abstract] [Full Text]
Miettinen, H., Gripentrog, J., Jesaitis, A. (2000). Chemotaxis of chinese hamster ovary cells expressing the human neutrophil formyl peptide receptor: role of signal transduction molecules and alpha5beta1 integrin. J. Cell Sci. 111: 1921-1928 [Abstract]
Gautam, N., Herwald, H., Hedqvist, P., Lindbom, L. (2000). Signaling via {beta}2 Integrins Triggers Neutrophil-dependent Alteration in Endothelial Barrier Function. J. Exp. Med. 191: 1829-1840 [Abstract] [Full Text]
Harler, M. B., Wakshull, E., Filardo, E. J., Albina, J. E., Reichner, J. S. (1999). Promotion of Neutrophil Chemotaxis Through Differential Regulation of {beta}1 and {beta}2 Integrins. J. Immunol. 162: 6792-6799 [Abstract] [Full Text]
Menegazzi, R., Busetto, S., Decleva, E., Cramer, R., Dri, P., Patriarca, P. (1999). Triggering of Chloride Ion Efflux from Human Neutrophils as a Novel Function of Leukocyte {beta}2 Integrins: Relationship with Spreading and Activation of the Respiratory Burst. J. Immunol. 162: 423-434 [Abstract] [Full Text]
Zheng, L., Eckerdal, J., Dimitrijevic, I., Andersson, T. (1997). Chemotactic Peptide-induced Activation of Ras in Human Neutrophils Is Associated with Inhibition of p120-GAP Activity. J. Biol. Chem. 272: 23448-23454 [Abstract] [Full Text]
Downey, G. P. (1997). Invited Editorial on "Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes". J. Appl. Physiol. 82: 1395-1396 [Abstract] [Full Text]
Garnotel, R., Monboisse, J.-C., Randoux, A., Haye, B., Borel, J. P. (1995). The Binding of Type I Collagen to Lymphocyte Function-associated Antigen (LFA) 1 Integrin Triggers the Respiratory Burst of Human Polymorphonuclear Neutrophils. J. Biol. Chem. 270: 27495-27503 [Abstract] [Full Text]
Meijne, A., Driessens, M., La Riviere, G, Casey, D, Feltkamp, C., Roos, E (1994). LFA-1 integrin redistribution during T-cell hybridoma invasion of hepatocyte cultures and manganese-induced adhesion to ICAM-1. J. Cell Sci. 107: 2557-2566 [Abstract]