Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion

doi:10.1083/jcb.127.6.1885

This Article

	PDF (Full Text)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Zimmerberg, J.
	Articles by Morris, S. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Zimmerberg, J.
	Articles by Morris, S. J.


Social Bookmarking

	What's this?

ARTICLES

Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion

J Zimmerberg, R Blumenthal, DP Sarkar, M Curran and SJ Morris
Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

The fusion of cells by influenza hemagglutinin (HA) is the best characterized example of protein-mediated membrane fusion. In simultaneous measurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane capacitance, next by a flux of fluorescent lipid, and finally by flux of aqueous fluorescent dye. Fusion pore conductance increased by small steps. A retardation of lipid and aqueous dyes occurred during fusion pore fluctuations. The flux of aqueous dye depended on the size of the molecule. The lack of movement of aqueous dyes while total fusion pore conductance increased suggests that initial HA-triggered fusion events are characterized by the opening of multiple small pores: the formation of a "sieve".
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Biswas, S., Yin, S.-R., Blank, P. S., Zimmerberg, J. (2008). Cholesterol Promotes Hemifusion and Pore Widening in Membrane Fusion Induced by Influenza Hemagglutinin. J. Gen. Physiol. 131: 503-513 [Abstract] [Full Text]
Wessels, L., Elting, M. W., Scimeca, D., Weninger, K. (2007). Rapid Membrane Fusion of Individual Virus Particles with Supported Lipid Bilayers. Biophys. J 93: 526-538 [Abstract] [Full Text]
Chernomordik, L. V., Zimmerberg, J., Kozlov, M. M. (2006). Membranes of the world unite!. J. Cell Biol. 175: 201-207 [Abstract] [Full Text]
Reese, C., Mayer, A. (2005). Transition from hemifusion to pore opening is rate limiting for vacuole membrane fusion. J. Cell Biol. 171: 981-990 [Abstract] [Full Text]
Li, Y., Han, X., Lai, A. L., Bushweller, J. H., Cafiso, D. S., Tamm, L. K. (2005). Membrane Structures of the Hemifusion-Inducing Fusion Peptide Mutant G1S and the Fusion-Blocking Mutant G1V of Influenza Virus Hemagglutinin Suggest a Mechanism for Pore Opening in Membrane Fusion. J. Virol. 79: 12065-12076 [Abstract] [Full Text]
Giraudo, C. G., Hu, C., You, D., Slovic, A. M., Mosharov, E. V., Sulzer, D., Melia, T. J., Rothman, J. E. (2005). SNAREs can promote complete fusion and hemifusion as alternative outcomes. J. Cell Biol. 170: 249-260 [Abstract] [Full Text]
Melikyan, G. B., Barnard, R. J. O., Abrahamyan, L. G., Mothes, W., Young, J. A. T. (2005). Imaging individual retroviral fusion events: From hemifusion to pore formation and growth. Proc. Natl. Acad. Sci. USA 102: 8728-8733 [Abstract] [Full Text]
Lizunov, V. A., Matsumoto, H., Zimmerberg, J., Cushman, S. W., Frolov, V. A. (2005). Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells. J. Cell Biol. 169: 481-489 [Abstract] [Full Text]
Kozlovsky, Y., Efrat, A., Siegel, D. A., Kozlov, M. M. (2004). Stalk Phase Formation: Effects of Dehydration and Saddle Splay Modulus. Biophys. J 87: 2508-2521 [Abstract] [Full Text]
Taraska, J. W., Almers, W. (2004). Bilayers merge even when exocytosis is transient. Proc. Natl. Acad. Sci. USA 101: 8780-8785 [Abstract] [Full Text]
Zavorotinskaya, T., Qian, Z., Franks, J., Albritton, L. M. (2004). A Point Mutation in the Binding Subunit of a Retroviral Envelope Protein Arrests Virus Entry at Hemifusion. J. Virol. 78: 473-481 [Abstract] [Full Text]
Frolov, V. A., Dunina-Barkovskaya, A. Y., Samsonov, A. V., Zimmerberg, J. (2003). Membrane Permeability Changes at Early Stages of Influenza Hemagglutinin-Mediated Fusion. Biophys. J 85: 1725-1733 [Abstract] [Full Text]
Greengard, O., Poltoratskaia, N., Leikina, E., Zimmerberg, J., Moscona, A. (2000). The Anti-Influenza Virus Agent 4-GU-DANA (Zanamivir) Inhibits Cell Fusion Mediated by Human Parainfluenza Virus and Influenza Virus HA. J. Virol. 74: 11108-11114 [Abstract] [Full Text]
Melikyan, G. B., Markosyan, R. M., Roth, M. G., Cohen, F. S. (2000). A Point Mutation in the Transmembrane Domain of the Hemagglutinin of Influenza Virus Stabilizes a Hemifusion Intermediate That Can Transit to Fusion. Mol. Biol. Cell 11: 3765-3775 [Abstract] [Full Text]
Gaudin, Y. (2000). Rabies Virus-induced Membrane Fusion Pathway. J. Cell Biol. 150: 601-612 [Abstract] [Full Text]
Leikina, E., Chernomordik, L. V. (2000). Reversible Merger of Membranes at the Early Stage of Influenza Hemagglutinin-mediated Fusion. Mol. Biol. Cell 11: 2359-2371 [Abstract] [Full Text]
Schmoranzer, J., Goulian, M., Axelrod, D., Simon, S. M. (2000). Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy. J. Cell Biol. 149: 23-32 [Abstract] [Full Text]
Markosyan, R. M., Cohen, F. S., Melikyan, G. B. (2000). The Lipid-anchored Ectodomain of Influenza Virus Hemagglutinin (GPI-HA) Is Capable of Inducing Nonenlarging Fusion Pores. Mol. Biol. Cell 11: 1143-1152 [Abstract] [Full Text]
Bonnafous, P., Stegmann, T. (2000). Membrane Perturbation and Fusion Pore Formation in Influenza Hemagglutinin-mediated Membrane Fusion. A NEW MODEL FOR FUSION. J. Biol. Chem. 275: 6160-6166 [Abstract] [Full Text]
Williams, R., Webb, W. (2000). Single granule pH cycling in antigen-induced mast cell secretion. J. Cell Sci. 113: 3839-3850 [Abstract]
Melikyan, G. B., Markosyan, R. M., Brener, S. A., Rozenberg, Y., Cohen, F. S. (2000). Role of the Cytoplasmic Tail of Ecotropic Moloney Murine Leukemia Virus Env Protein in Fusion Pore Formation. J. Virol. 74: 447-455 [Abstract] [Full Text]
Mordue, D. G., Desai, N., Dustin, M., Sibley, L. D. (1999). Invasion by Toxoplasma gondii Establishes a Moving Junction That Selectively Excludes Host Cell Plasma Membrane Proteins on the Basis of Their Membrane Anchoring. J. Exp. Med. 190: 1783-1792 [Abstract] [Full Text]
Kingsley, D. H., Behbahani, A., Rashtian, A., Blissard, G. W., Zimmerberg, J. (1999). A Discrete Stage of Baculovirus GP64-mediated Membrane Fusion. Mol. Biol. Cell 10: 4191-4200 [Abstract] [Full Text]
Qiao, H., Armstrong, R. T., Melikyan, G. B., Cohen, F. S., White, J. M. (1999). A Specific Point Mutant at Position 1 of the Influenza Hemagglutinin Fusion Peptide Displays a Hemifusion Phenotype. Mol. Biol. Cell 10: 2759-2769 [Abstract] [Full Text]
Melikyan, G. B., Lin, S., Roth, M. G., Cohen, F. S. (1999). Amino Acid Sequence Requirements of the Transmembrane and Cytoplasmic Domains of Influenza Virus Hemagglutinin for Viable Membrane Fusion. Mol. Biol. Cell 10: 1821-1836 [Abstract] [Full Text]
Markovic, I., Pulyaeva, H., Sokoloff, A., Chernomordik, L. V. (1998). Membrane Fusion Mediated by Baculovirus gp64 Involves Assembly of Stable gp64 Trimers into Multiprotein Aggregates. J. Cell Biol. 143: 1155-1166 [Abstract] [Full Text]
Lee, J., Lentz, B. R. (1998). Secretory and viral fusion may share mechanistic events with fusion between curved lipid bilayers. Proc. Natl. Acad. Sci. USA 95: 9274-9279 [Abstract] [Full Text]
Chernomordik, L. V., Frolov, V. A., Leikina, E., Bronk, P., Zimmerberg, J. (1998). The Pathway of Membrane Fusion Catalyzed by Influenza Hemagglutinin: Restriction of Lipids, Hemifusion, and Lipidic Fusion Pore Formation. J. Cell Biol. 140: 1369-1382 [Abstract] [Full Text]
Munoz-Barroso, I., Durell, S., Sakaguchi, K., Appella, E., Blumenthal, R. (1998). Dilation of the Human Immunodeficiency Virus-1 Envelope Glycoprotein Fusion Pore Revealed by the Inhibitory Action of a Synthetic Peptide from gp41. J. Cell Biol. 140: 315-323 [Abstract] [Full Text]
Chanturiya, A., Chernomordik, L. V., Zimmerberg, J. (1997). Flickering fusion pores comparable with initial exocytotic pores occur in protein-free�phospholipid�bilayers. Proc. Natl. Acad. Sci. USA 94: 14423-14428 [Abstract] [Full Text]
Melikyan, G. B., Brener, S. A., Ok, D. C., Cohen, F. S. (1997). Inner but Not Outer Membrane Leaflets Control the Transition from Glycosylphosphatidylinositol-anchored Influenza Hemagglutinin-induced Hemifusion to Full Fusion. J. Cell Biol. 136: 995-1005 [Abstract] [Full Text]
Chernomordik, L. V., Leikina, E., Frolov, V., Bronk, P., Zimmerberg, J. (1997). An Early Stage of Membrane Fusion Mediated by the Low pH Conformation of Influenza Hemagglutinin Depends upon Membrane Lipids. J. Cell Biol. 136: 81-93 [Abstract] [Full Text]