Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication

doi:10.1083/jcb.132.5.887

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Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication

AD Donaldson and JV Kilmartin
MRC Laboratory of Molecular Biology, Cambridge, England.

The 42-kD component of the S. cerevisiae spindle pole body (SPB) localizes to the electron-dense central plaque of the SPB. We have cloned the corresponding gene SPC42 (spindle pole component) and show that it is essential. Seven temperature-sensitive (ts) mutants in SPC42 were prepared by error-prone PCR. We found that a change to a proline residue in a potential coiled-coil region of Spc42p was responsible for the ts phenotype in at least three alleles, suggesting that formation of the coiled-coil is essential in normal function. The mutant cells showed a phenotype of predominantly single or bilobed SPBs often with an accumulation of unstructured electron-dense material associated with the bridge structure adjacent to the SPB. This phenotype suggests a defect in SPB duplication. This was confirmed by examining synchronized mutant cells that lose viability when SPB duplication is attempted. Spc42p is a phosphoprotein which shows some cell cycle-regulated phosphorylation. Overexpression of Spc42p causes the formation of a disc- or dome-shaped polymer composed of phosphorylated Spc42p, which is attached to the central plaque and associated with the outer nuclear membrane. Taken together, these data suggest that Spc42p forms a polymeric layer at the periphery of the SPB central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.
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Kitagaki, H., Ito, K., Shimoi, H. (2004). A Temperature-Sensitive dcw1 Mutant of Saccharomyces cerevisiae Is Cell Cycle Arrested with Small Buds Which Have Aberrant Cell Walls. Eukaryot Cell 3: 1297-1306 [Abstract] [Full Text]
Melloy, P. G., Holloway, S. L. (2004). Changes in the Localization of the Saccharomyces cerevisiae Anaphase-Promoting Complex Upon Microtubule Depolymerization and Spindle Checkpoint Activation. Genetics 167: 1079-1094 [Abstract] [Full Text]
Pereira, G., Schiebel, E. (2003). Separase Regulates INCENP-Aurora B Anaphase Spindle Function Through Cdc14. Science 302: 2120-2124 [Abstract] [Full Text]
Kilmartin, J. V. (2003). Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. JCB 162: 1211-1221 [Abstract] [Full Text]
Yoder, T. J., Pearson, C. G., Bloom, K., Davis, T. N. (2003). The Saccharomyces cerevisiae Spindle Pole Body Is a Dynamic Structure. Mol. Biol. Cell 14: 3494-3505 [Abstract] [Full Text]
Jaspersen, S. L., Giddings, T. H. Jr., Winey, M. (2002). Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p. JCB 159: 945-956 [Abstract] [Full Text]
Gentry, M. S., Hallberg, R. L. (2002). Localization of Saccharomyces cerevisiae Protein Phosphatase 2A Subunits throughout Mitotic Cell Cycle. Mol. Biol. Cell 13: 3477-3492 [Abstract] [Full Text]
Pereira, G., Manson, C., Grindlay, J., Schiebel, E. (2002). Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. JCB 157: 367-379 [Abstract] [Full Text]
Vinh, D. B.N., Kern, J. W., Hancock, W. O., Howard, J., Davis, T. N. (2002). Reconstitution and Characterization of Budding Yeast gamma -Tubulin Complex. Mol. Biol. Cell 13: 1144-1157 [Abstract] [Full Text]
Castillo, A. R., Meehl, J. B., Morgan, G., Schutz-Geschwender, A., Winey, M. (2002). The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. JCB 156: 453-465 [Abstract] [Full Text]
Heun, P., Laroche, T., Shimada, K., Furrer, P., Gasser, S. M. (2001). Chromosome Dynamics in the Yeast Interphase Nucleus. Science 294: 2181-2186 [Abstract] [Full Text]
Luca, F. C., Mody, M., Kurischko, C., Roof, D. M., Giddings, T. H., Winey, M. (2001). Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit. Mol. Cell. Biol. 21: 6972-6983 [Abstract] [Full Text]
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Schaerer, F., Morgan, G., Winey, M., Philippsen, P. (2001). Cnm67p Is a Spacer Protein of the Saccharomyces cerevisiae Spindle Pole Body Outer Plaque. Mol. Biol. Cell 12: 2519-2533 [Abstract] [Full Text]
Newman, J. R. S., Wolf, E., Kim, P. S. (2000). From the Cover: A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 97: 13203-13208 [Abstract] [Full Text]
Chang, L., Gould, K. L. (2000). Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast. Proc. Natl. Acad. Sci. USA 97: 5249-5254 [Abstract] [Full Text]
Middendorp, S., Kuntziger, T., Abraham, Y., Holmes, S., Bordes, N., Paintrand, M., Paoletti, A., Bornens, M. (2000). A Role for Centrin 3 in Centrosome Reproduction. JCB 148: 405-416 [Abstract] [Full Text]
Graf, R, Daunderer, C, Schliwa, M (2000). Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication. J. Cell Sci. 113: 1747-1758 [Abstract]
Song, S., Grenfell, T. Z., Garfield, S., Erikson, R. L., Lee, K. S. (2000). Essential Function of the Polo Box of Cdc5 in Subcellular Localization and Induction of Cytokinetic Structures. Mol. Cell. Biol. 20: 286-298 [Abstract] [Full Text]
Muñoz-Centeno, M. d. l. C., McBratney, S., Monterrosa, A., Byers, B., Mann, C., Winey, M. (1999). Saccharomyces cerevisiae MPS2 Encodes a Membrane Protein Localized at the Spindle Pole Body and the Nuclear Envelope. Mol. Biol. Cell 10: 2393-2406 [Abstract] [Full Text]
O'Toole, E. T., Winey, M., McIntosh, J. R. (1999). High-Voltage Electron Tomography of Spindle Pole Bodies and Early Mitotic Spindles in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 10: 2017-2031 [Abstract] [Full Text]
Elliott, S., Knop, M., Schlenstedt, G., Schiebel, E. (1999). Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proc. Natl. Acad. Sci. USA 96: 6205-6210 [Abstract] [Full Text]
Adams, I. R., Kilmartin, J. V. (1999). Localization of Core Spindle Pole Body (SPB) Components during SPB Duplication in Saccharomyces cerevisiae. JCB 145: 809-823 [Abstract] [Full Text]
Chial, H. J., Rout, M. P., Giddings, T. H. Jr., Winey, M. (1998). Saccharomyces cerevisiae Ndc1p Is a Shared Component of Nuclear Pore Complexes and Spindle Pole Bodies. JCB 143: 1789-1800 [Abstract] [Full Text]
Cheng, L., Hunke, L., Hardy, C. F.�J. (1998). Cell Cycle Regulation of the Saccharomyces cerevisiae Polo-Like Kinase Cdc5p. Mol. Cell. Biol. 18: 7360-7370 [Abstract] [Full Text]
Spellman, P. T., Sherlock, G., Zhang, M. Q., Iyer, V. R., Anders, K., Eisen, M. B., Brown, P. O., Botstein, D., Futcher, B. (1998). Comprehensive Identification of Cell Cycle-regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization. Mol. Biol. Cell 9: 3273-3297 [Abstract] [Full Text]
Chen, X. P., Yin, H., Huffaker, T. C. (1998). The Yeast Spindle Pole Body Component Spc72p Interacts with Stu2p and Is Required for Proper Microtubule Assembly. JCB 141: 1169-1179 [Abstract] [Full Text]
Wigge, P. A., Jensen, O. N., Holmes, S., Soues, S., Mann, M., Kilmartin, J. V. (1998). Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. JCB 141: 967-977 [Abstract] [Full Text]
Schutz, A. R., Winey, M. (1998). New Alleles of the Yeast MPS1 Gene Reveal Multiple Requirements in Spindle Pole Body Duplication. Mol. Biol. Cell 9: 759-774 [Abstract] [Full Text]
Pereira, G., Knop, M., Schiebel, E. (1998). Spc98p Directs the Yeast gamma -Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body. Mol. Biol. Cell 9: 775-793 [Abstract] [Full Text]
Soues, S, Adams, I. (1998). SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae. J. Cell Sci. 111: 2809-2818 [Abstract]
Madeo, F., Schlauer, J., Zischka, H., Mecke, D., Fröhlich, K.-U. (1998). Tyrosine Phosphorylation Regulates Cell Cycle-dependent Nuclear Localization of Cdc48p. Mol. Biol. Cell 9: 131-141 [Abstract] [Full Text]
Tassin, A., Celati, C, Paintrand, M, Bornens, M (1997). Identification of an Spc110p-related protein in vertebrates. J. Cell Sci. 110: 2533-2545 [Abstract]
Zarzov, P, Boucherie, H, Mann, C (1997). A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J. Cell Sci. 110: 1879-1891 [Abstract]
Spang, A, Grein, K, Schiebel, E (1996). The spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body. J. Cell Sci. 109: 2229-2237 [Abstract]
Castillo, A. R., Meehl, J. B., Morgan, G., Schutz-Geschwender, A., Winey, M. (2002). The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. JCB 156: 453-465 [Abstract] [Full Text]
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