J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/02/895/12 $2.00
Volume 136, Number 4, February 24, 1997 895-906

Chicken Acidic Leucine-rich EGF-like Domain Containing Brain Protein (CALEB), a Neural Member of the EGF Family of Differentiation Factors, Is Implicated in Neurite Formation

Stefan Schumacher,^* Hansjürgen Volkmer,^* Fritz Buck, Albrecht Otto,^* Attila Tárnok,^§ Siegfried Roth, and Fritz G. Rathjen^*

^* Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany;  Institut für Zellbiochemie, D-20246 Hamburg, Germany; and ^§ Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany

Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH₂-terminal glycosylation region, a leucine-proline-rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.

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