J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/05/581/13 $2.00
Volume 137, Number 3, May 5, 1997 581-593

p53/58 Binds COPI and Is Required for Selective Transport through the Early Secretory Pathway

Ellen J. Tisdale, Helen Plutner, Jeanne Matteson, and William E. Balch

The Scripps Research Institute, Departments of Cell and Molecular Biology, La Jolla, California 92037

p53/58 is a transmembrane protein that continuously recycles between the ER and pre-Golgi intermediates composed of vesicular-tubular clusters (VTCs) found in the cell periphery and at the cis face of the Golgi complex. We have generated an antibody that uniquely recognizes the p53/58 cytoplasmic tail. Here we present evidence that this antibody arrests the anterograde transport of vesicular stomatitis virus glycoprotein and leads to the accumulation of p58 in preGolgi intermediates. Consistent with a role for the KKXX retrieval motif found at the cytoplasmic carboxyl terminus of p53/58 in retrograde traffic, inhibition of transport through VTCs correlates with the ability of the antibody to block recruitment of COPI coats to the p53/58 cytoplasmic tail and to p53/58-containing membranes. We suggest that p53/58 function may be required for the coupled exchange of COPII for COPI coats during segregation of anterograde and retrograde transported proteins.

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