J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/06/1581/08 $2.00
Volume 137, Number 7, June 30, 1997 1581-1588

Human Bcl-2 Reverses Survival Defects in Yeast Lacking Superoxide Dismutase and Delays Death of Wild-Type Yeast

Valter D. Longo,^* Lisa M. Ellerby, Dale E. Bredesen,^§ Joan S. Valentine,^* and Edith B. Gralla^*

^* Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095-1569;  Program on Aging, The Burnham Institute, La Jolla, California 92037; and ^§ Neuroscience Department, University of California at San Diego, La Jolla, California 92093

We expressed the human anti-apoptotic protein, Bcl-2, in Saccharomyces cerevisiae to investigate its effects on antioxidant protection and stationary phase survival. Yeast lacking copper-zinc superoxide dismutase (sod1) show a profound defect in entry into and survival during stationary phase even under conditions optimal for survival of wild-type strains (incubation in water after stationary phase is reached). Expression of Bcl-2 in the sod1 strain caused a large improvement in viability at entry into stationary phase, as well as increased resistance to 100% oxygen and increased catalase activity. In addition, Bcl-2 expression reduced mutation frequency in both wild-type and sod1 strains. In another set of experiments, wild-type yeast incubated in expired minimal medium instead of water lost viability quickly; expression of Bcl-2 significantly delayed this stationary phase death. Our results demonstrate that Bcl-2 has activities in yeast that are similar to activities it is known to possess in mammalian cells: (a) stimulation of antioxidant protection and (b) delay of processes leading to cell death.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Hauptmann, P., Lehle, L. (2008). Kex1 Protease Is Involved in Yeast Cell Death Induced by Defective N-Glycosylation, Acetic Acid, and Chronological Aging. J. Biol. Chem. 283: 19151-19163 [Abstract] [Full Text]  
• Siskind, L. J., Feinstein, L., Yu, T., Davis, J. S., Jones, D., Choi, J., Zuckerman, J. E., Tan, W., Hill, R. B., Hardwick, J. M., Colombini, M. (2008). Anti-apoptotic Bcl-2 Family Proteins Disassemble Ceramide Channels. J. Biol. Chem. 283: 6622-6630 [Abstract] [Full Text]  
• Murakami, C. J., Burtner, C. R., Kennedy, B. K., Kaeberlein, M. (2008). A Method for High-Throughput Quantitative Analysis of Yeast Chronological Life Span. J. Gerontol. A Biol. Sci. Med. Sci. 63: 113-121 [Abstract] [Full Text]  
• Madia, F., Gattazzo, C., Wei, M., Fabrizio, P., Burhans, W. C., Weinberger, M., Galbani, A., Smith, J. R., Nguyen, C., Huey, S., Comai, L., Longo, V. D. (2008). Longevity mutation in SCH9 prevents recombination errors and premature genomic instability in a Werner/Bloom model system. J. Cell Biol. 180: 67-81 [Abstract] [Full Text]  
• Buttner, S., Eisenberg, T., Herker, E., Carmona-Gutierrez, D., Kroemer, G., Madeo, F. (2006). Why yeast cells can undergo apoptosis: death in times of peace, love, and war. J. Cell Biol. 175: 521-525 [Abstract] [Full Text]  
• Yoshida, A., Takemura, H., Inoue, H., Miyashita, T., Ueda, T. (2006). Inhibition of Glutathione Synthesis Overcomes Bcl-2-Mediated Topoisomerase Inhibitor Resistance and Induces Nonapoptotic Cell Death via Mitochondrial-Independent Pathway. Cancer Res. 66: 5772-5780 [Abstract] [Full Text]  
• Chen, Q., Thorpe, J., Keller, J. N. (2005). {alpha}-Synuclein Alters Proteasome Function, Protein Synthesis, and Stationary Phase Viability. J. Biol. Chem. 280: 30009-30017 [Abstract] [Full Text]  
• Ivanovska, I., Hardwick, J. M. (2005). Viruses activate a genetically conserved cell death pathway in a unicellular organism. J. Cell Biol. 170: 391-399 [Abstract] [Full Text]  
• Davenport, R. J. (2004). Culture Clash. Sci Aging Knowl Environ 2004: ns9-ns9 [Abstract] [Full Text]  
• Fannjiang, Y., Cheng, W.-C., Lee, S. J., Qi, B., Pevsner, J., McCaffery, J. M., Hill, R. B., Basanez, G., Hardwick, J. M. (2004). Mitochondrial fission proteins regulate programmed cell death in yeast. Genes Dev. 18: 2785-2797 [Abstract] [Full Text]  
• Fabrizio, P., Battistella, L., Vardavas, R., Gattazzo, C., Liou, L.-L., Diaspro, A., Dossen, J. W., Gralla, E. B., Longo, V. D. (2004). Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae. J. Cell Biol. 166: 1055-1067 [Abstract] [Full Text]  
• Sampayo, J. N., Lithgow, G. J. (2004). S.W.A.T.--SOD Weapons and Tactics. Sci Aging Knowl Environ 2004: pe27-pe27 [Abstract] [Full Text]  
• Herker, E., Jungwirth, H., Lehmann, K. A., Maldener, C., Frohlich, K.-U., Wissing, S., Buttner, S., Fehr, M., Sigrist, S., Madeo, F. (2004). Chronological aging leads to apoptosis in yeast. J. Cell Biol. 164: 501-507 [Abstract] [Full Text]  
• Archibald, F. (2003). Oxygen toxicity and the health and survival of eukaryote cells: A new piece is added to the puzzle. Proc. Natl. Acad. Sci. USA 100: 10141-10143 [Full Text]  
• Bitterman, K. J., Medvedik, O., Sinclair, D. A. (2003). Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin. Microbiol. Mol. Biol. Rev. 67: 376-399 [Abstract] [Full Text]  
• Fabrizio, P., Liou, L.-L., Moy, V. N., Diaspro, A., Selverstone Valentine, J., Gralla, E. B., Longo, V. D. (2003). SOD2 Functions Downstream of Sch9 to Extend Longevity in Yeast. Genetics 163: 35-46 [Abstract] [Full Text]  
• Vander Heiden, M. G., Choy, J. S., VanderWeele, D. J., Brace, J. L., Harris, M. H., Bauer, D. E., Prange, B., Kron, S. J., Thompson, C. B., Rudin, C. M. (2002). Bcl-xL Complements Saccharomyces cerevisiae Genes That Facilitate the Switch from Glycolytic to Oxidative Metabolism. J. Biol. Chem. 277: 44870-44876 [Abstract] [Full Text]  
• Poliakova, D., Sokolikova, B., Kolarov, J., Sabova, L'u. (2002). The antiapoptotic protein Bcl-xL prevents the cytotoxic effect of Bax, but not Bax-induced formation of reactive oxygen species, in Kluyveromyces lactis. Microbiology 148: 2789-2795 [Abstract] [Full Text]  
• Kaeberlein, M., McVey, M., Guarente, L. (2001). Using Yeast to Discover the Fountain of Youth. Sci Aging Knowl Environ 2001: pe1-1 [Abstract] [Full Text]  
• Ludovico, P., Sousa, M. J., Silva, M. T., Leao, C., Corte-Real, M. (2001). Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid. Microbiology 147: 2409-2415 [Abstract] [Full Text]  
• Lewis, K. (2000). Programmed Death in Bacteria. Microbiol. Mol. Biol. Rev. 64: 503-514 [Abstract] [Full Text]  
• Ashrafi, K., Sinclair, D., Gordon, J. I., Guarente, L. (1999). Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 96: 9100-9105 [Abstract] [Full Text]  
• Madeo, F., Frohlich, E., Ligr, M., Grey, M., Sigrist, S. J., Wolf, D. H., Frohlich, K.-U. (1999). Oxygen Stress: A Regulator of Apoptosis in Yeast. J. Cell Biol. 145: 757-767 [Abstract] [Full Text]  
• Matsuyama, S., Schendel, S. L., Xie, Z., Reed, J. C. (1998). Cytoprotection by Bcl-2 Requires the Pore-forming alpha 5 and alpha 6 Helices. J. Biol. Chem. 273: 30995-31001 [Abstract] [Full Text]  
• Fabrizio, P., Pozza, F., Pletcher, S. D., Gendron, C. M., Longo, V. D. (2001). Regulation of Longevity and Stress Resistance by Sch9 in Yeast. Science 292: 288-290 [Abstract] [Full Text]  
• Sturtz, L. A., Diekert, K., Jensen, L. T., Lill, R., Culotta, V. C. (2001). A Fraction of Yeast Cu,Zn-Superoxide Dismutase and Its Metallochaperone, CCS, Localize to the Intermembrane Space of Mitochondria. A PHYSIOLOGICAL ROLE FOR SOD1 IN GUARDING AGAINST MITOCHONDRIAL OXIDATIVE DAMAGE. J. Biol. Chem. 276: 38084-38089 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents