J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/07/65/16 $2.00
Volume 138, Number 1, July 14, 1997 65-80

A Novel Class of RanGTP Binding Proteins

Dirk Görlich,^* Marylena Dabrowski,^* F. Ralf Bischoff,^§ Ulrike Kutay,^* Peer Bork, Enno Hartmann, Siegfried Prehn,^¶ and Elisa Izaurralde^**

^* Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany;  Max-Delbrück-Zentrum für Molekulare Medizin, 13122 Berlin-Buch, Germany; ^§ Abteilung Molekulare Biologie der Mitose, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany;  European Molecular Biology Laboratory, 69117 Heidelberg, Germany; ^¶ Institut für Biochemie, Humboldt Universität Berlin, 10115 Berlin, Germany; and ^** University of Geneva, Department of Molecular Biology, CH-1211 Geneva 4, Switzerland

The importin-/ complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. Although Ran has been implicated also in a variety of other processes, such as cell cycle progression, a direct function of Ran has so far only been demonstrated for importin-mediated nuclear import. We have now identified an entire class of ~20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-. We have confirmed specific RanGTP binding for some of them, namely for two novel factors, RanBP7 and RanBP8, for CAS, Pse1p, and Msn5p, and for the cell cycle regulator Cse1p from Saccharomyces cerevisiae. We have studied RanBP7 in more detail. Similar to importin-, it prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP. RanBP7 binds directly to nuclear pore complexes where it competes for binding sites with importin-, transportin, and apparently also with the mediators of mRNA and U snRNA export. Furthermore, we provide evidence for a Ran-dependent transport cycle of RanBP7 and demonstrate that RanBP7 can cross the nuclear envelope rapidly and in both directions. On the basis of these results, we propose that RanBP7 might represent a nuclear transport factor that carries an as yet unknown cargo, which could apply as well for this entire class of related RanGTP-binding proteins.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Yao, X., Chen, X., Cottonham, C., Xu, L. (2008). Preferential Utilization of Imp7/8 in Nuclear Import of Smads. J. Biol. Chem. 283: 22867-22874 [Abstract] [Full Text]  
• Quan, Y., Ji, Z.-L., Wang, X., Tartakoff, A. M., Tao, T. (2008). Evolutionary and Transcriptional Analysis of Karyopherin {beta} Superfamily Proteins. Mol. Cell. Proteomics 7: 1254-1269 [Abstract] [Full Text]  
• Saijou, E., Itoh, T., Kim, K.-W., Iemura, S.-i., Natsume, T., Miyajima, A. (2007). Nucleocytoplasmic Shuttling of the Zinc Finger Protein EZI Is Mediated by Importin-7-dependent Nuclear Import and CRM1-independent Export Mechanisms. J. Biol. Chem. 282: 32327-32337 [Abstract] [Full Text]  
• James, B. P., Bunch, T. A., Krishnamoorthy, S., Perkins, L. A., Brower, D. L. (2007). Nuclear Localization of the ERK MAP Kinase Mediated by Drosophila {alpha}PS2betaPS Integrin and Importin-7. Mol. Biol. Cell 18: 4190-4199 [Abstract] [Full Text]  
• Xu, L., Yao, X., Chen, X., Lu, P., Zhang, B., Ip, Y. T. (2007). Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. J. Cell Biol. 178: 981-994 [Abstract] [Full Text]  
• Ao, Z., Huang, G., Yao, H., Xu, Z., Labine, M., Cochrane, A. W., Yao, X. (2007). Interaction of Human Immunodeficiency Virus Type 1 Integrase with Cellular Nuclear Import Receptor Importin 7 and Its Impact on Viral Replication. J. Biol. Chem. 282: 13456-13467 [Abstract] [Full Text]  
• Wohlwend, D., Strasser, A., Dickmanns, A., Doenecke, D., Ficner, R. (2007). Thermodynamic Analysis of H1 Nuclear Import: RECEPTOR TUNING OF IMPORTINbeta/IMPORTIN7. J. Biol. Chem. 282: 10707-10719 [Abstract] [Full Text]  
• Pepple, K. L., Anderson, A. E., Frankfort, B. J., Mardon, G. (2007). A Genetic Screen in Drosophila for Genes Interacting With senseless During Neuronal Development Identifies the Importin moleskin. Genetics 175: 125-141 [Abstract] [Full Text]  
• Fu, X., Choi, Y.-K., Qu, D., Yu, Y., Cheung, N. S., Qi, R. Z. (2006). Identification of Nuclear Import Mechanisms for the Neuronal Cdk5 Activator. J. Biol. Chem. 281: 39014-39021 [Abstract] [Full Text]  
• Arnold, M., Nath, A., Hauber, J., Kehlenbach, R. H. (2006). Multiple Importins Function as Nuclear Transport Receptors for the Rev Protein of Human Immunodeficiency Virus Type 1. J. Biol. Chem. 281: 20883-20890 [Abstract] [Full Text]  
• Ribbeck, K., Groen, A. C., Santarella, R., Bohnsack, M. T., Raemaekers, T., Kocher, T., Gentzel, M., Gorlich, D., Wilm, M., Carmeliet, G., Mitchison, T. J., Ellenberg, J., Hoenger, A., Mattaj, I. W. (2006). NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules. Mol. Biol. Cell 17: 2646-2660 [Abstract] [Full Text]  
• Arnold, M., Nath, A., Wohlwend, D., Kehlenbach, R. H. (2006). Transportin Is a Major Nuclear Import Receptor for c-Fos: A NOVEL MODE OF CARGO INTERACTION. J. Biol. Chem. 281: 5492-5499 [Abstract] [Full Text]  
• Erkmann, J. A., Wagner, E. J., Dong, J., Zhang, Y., Kutay, U., Marzluff, W. F. (2005). Nuclear Import of the Stem-Loop Binding Protein and Localization during the Cell Cycle. Mol. Biol. Cell 16: 2960-2971 [Abstract] [Full Text]  
• Daelemans, D., Costes, S. V., Lockett, S., Pavlakis, G. N. (2005). Kinetic and Molecular Analysis of Nuclear Export Factor CRM1 Association with Its Cargo In Vivo. Mol. Cell. Biol. 25: 728-739 [Abstract] [Full Text]  
• Freedman, N. D., Yamamoto, K. R. (2004). Importin 7 and Importin {alpha}/Importin {beta} Are Nuclear Import Receptors for the Glucocorticoid Receptor. Mol. Biol. Cell 15: 2276-2286 [Abstract] [Full Text]  
• DLAKIC, M., TOLLERVEY, D. (2004). The Noc proteins involved in ribosome synthesis and export contain divergent HEAT repeats. RNA 10: 351-354 [Abstract] [Full Text]  
• Fukuhara, N., Fernandez, E., Ebert, J., Conti, E., Svergun, D. (2004). Conformational Variability of Nucleo-cytoplasmic Transport Factors. J. Biol. Chem. 279: 2176-2181 [Abstract] [Full Text]  
• Cushman, I., Stenoien, D., Moore, M. S. (2004). The Dynamic Association of RCC1 with Chromatin Is Modulated by Ran-dependent Nuclear Transport. Mol. Biol. Cell 15: 245-255 [Abstract] [Full Text]  
• Ueta, R., Fukunaka, A., Yamaguchi-Iwai, Y. (2003). Pse1p Mediates the Nuclear Import of the Iron-responsive Transcription Factor Aft1p in Saccharomyces cerevisiae. J. Biol. Chem. 278: 50120-50127 [Abstract] [Full Text]  
• Hakata, Y., Yamada, M., Shida, H. (2003). A Multifunctional Domain in Human CRM1 (Exportin 1) Mediates RanBP3 Binding and Multimerization of Human T-Cell Leukemia Virus Type 1 Rex Protein. Mol. Cell. Biol. 23: 8751-8761 [Abstract] [Full Text]  
• Roth, P., Xylourgidis, N., Sabri, N., Uv, A., Fornerod, M., Samakovlis, C. (2003). The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export. J. Cell Biol. 163: 701-706 [Abstract] [Full Text]  
• Penalva, L. O. F., Sanchez, L. (2003). RNA Binding Protein Sex-Lethal (Sxl) and Control of Drosophila Sex Determination and Dosage Compensation. Microbiol. Mol. Biol. Rev. 67: 343-359 [Abstract] [Full Text]  
• Li, J., Chen, X. (2003). PAUSED, a Putative Exportin-t, Acts Pleiotropically in Arabidopsis Development But Is Dispensable for Viability. Plant Physiol. 132: 1913-1924 [Abstract] [Full Text]  
• Thomas, F., Kutay, U. (2003). Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway. J. Cell Sci. 116: 2409-2419 [Abstract] [Full Text]  
• Queralt, E., Igual, J. C. (2003). Cell Cycle Activation of the Swi6p Transcription Factor Is Linked to Nucleocytoplasmic Shuttling. Mol. Cell. Biol. 23: 3126-3140 [Abstract] [Full Text]  
• Bollman, K. M., Aukerman, M. J., Park, M.-Y., Hunter, C., Berardini, T. Z., Poethig, R. S. (2003). HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis. Development 130: 1493-1504 [Abstract] [Full Text]  
• Anway, M. D., Li, Y., Ravindranath, N., Dym, M., Griswold, M. D. (2003). Expression of Testicular Germ Cell Genes Identified by Differential Display Analysis. J Androl 24: 173-184 [Abstract] [Full Text]  
• Ortega, A., Niksic, M., Bachi, A., Wilm, M., Sanchez, L., Hastie, N., Valcarcel, J. (2003). Biochemical Function of Female-Lethal (2)D/Wilms' Tumor Suppressor-1-associated Proteins in Alternative Pre-mRNA Splicing. J. Biol. Chem. 278: 3040-3047 [Abstract] [Full Text]  
• Daelemans, D., Afonina, E., Nilsson, J., Werner, G., Kjems, J., De Clercq, E., Pavlakis, G. N., Vandamme, A.-M. (2002). A synthetic HIV-1 Rev inhibitor interfering with the CRM1-mediated nuclear export. Proc. Natl. Acad. Sci. USA 99: 14440-14445 [Abstract] [Full Text]  
• Qutob, M. S., Bhattacharjee, R. N., Pollari, E., Yee, S. P., Torchia, J. (2002). Microtubule-Dependent Subcellular Redistribution of the Transcriptional Coactivator p/CIP. Mol. Cell. Biol. 22: 6611-6626 [Abstract] [Full Text]  
• Ferrezuelo, F., Steiner, B., Aldea, M., Futcher, B. (2002). Biogenesis of Yeast Telomerase Depends on the Importin Mtr10. Mol. Cell. Biol. 22: 6046-6055 [Abstract] [Full Text]  
• Bauerle, M., Doenecke, D., Albig, W. (2002). The Requirement of H1 Histones for a Heterodimeric Nuclear Import Receptor. J. Biol. Chem. 277: 32480-32489 [Abstract] [Full Text]  
• Feng, W., Hopper, A. K. (2002). A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 99: 5412-5417 [Abstract] [Full Text]  
• Jackman, M., Kubota, Y., den Elzen, N., Hagting, A., Pines, J. (2002). Cyclin A- and Cyclin E-Cdk Complexes Shuttle between the Nucleus and the Cytoplasm. Mol. Biol. Cell 13: 1030-1045 [Abstract] [Full Text]  
• Plafker, S. M., Macara, I. G. (2002). Ribosomal Protein L12 Uses a Distinct Nuclear Import Pathway Mediated by Importin 11. Mol. Cell. Biol. 22: 1266-1275 [Abstract] [Full Text]  
• Huber, J., Dickmanns, A., Luhrmann, R. (2002). The importin-{beta} binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro. J. Cell Biol. 156: 467-479 [Abstract] [Full Text]  
• Mosammaparast, N., Guo, Y., Shabanowitz, J., Hunt, D. F., Pemberton, L. F. (2002). Pathways Mediating the Nuclear Import of Histones H3 and H4 in Yeast. J. Biol. Chem. 277: 862-868 [Abstract] [Full Text]  
• Macara, I. G. (2001). Transport into and out of the Nucleus. Microbiol. Mol. Biol. Rev. 65: 570-594 [Abstract] [Full Text]  
• Bera, T. K., Bera, J., Brinkmann, U., Tessarollo, L., Pastan, I. (2001). Cse1l Is Essential for Early Embryonic Growth and Development. Mol. Cell. Biol. 21: 7020-7024 [Abstract] [Full Text]  
• Denning, D., Mykytka, B., Allen, N. P.C., Huang, L., Al Burlingame, , Rexach, M. (2001). The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex. J. Cell Biol. 154: 937-950 [Abstract] [Full Text]  
• Polizotto, R. S., Cyert, M. S. (2001). Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5p. J. Cell Biol. 154: 951-960 [Abstract] [Full Text]  
• Zenklusen, D., Vinciguerra, P., Strahm, Y., Stutz, F. (2001). The Yeast hnRNP-Like Proteins Yra1p and Yra2p Participate in mRNA Export through Interaction with Mex67p. Mol. Cell. Biol. 21: 4219-4232 [Abstract] [Full Text]  
• Mosammaparast, N., Jackson, K. R., Guo, Y., Brame, C. J., Shabanowitz, J., Hunt, D. F., Pemberton, L. F. (2001). Nuclear Import of Histone H2A and H2B Is Mediated by a Network of Karyopherins. J. Cell Biol. 153: 251-262 [Abstract] [Full Text]  
• Maurer, P., Redd, M., Solsbacher, J., Bischoff, F. R., Greiner, M., Podtelejnikov, A. V., Mann, M., Stade, K., Weis, K., Schlenstedt, G. (2001). The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p). Mol. Biol. Cell 12: 539-549 [Abstract] [Full Text]  
• Yoshida, K., Blobel, G. (2001). The Karyopherin Kap142p/Msn5p Mediates Nuclear Import and Nuclear Export of Different Cargo Proteins. J. Cell Biol. 152: 729-740 [Abstract] [Full Text]  
• Dean, K. A., von Ahsen, O., Gorlich, D., Fried, H. M. (2001). Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. J. Cell Sci. 114: 3479-3485 [Abstract] [Full Text]  
• Holaska, J. M., Black, B. E., Love, D. C., Hanover, J. A., Leszyk, J., Paschal, B. M. (2001). Calreticulin Is a Receptor for Nuclear Export. J. Cell Biol. 152: 127-140 [Abstract] [Full Text]  
• Lorenzen, J., Baker, S., Denhez, F, Melnick, M., Brower, D., Perkins, L. (2001). Nuclear import of activated D-ERK by DIM-7, an importin family member encoded by the gene moleskin. Development 128: 1403-1414 [Abstract]  
• Takai, Y., Sasaki, T., Matozaki, T. (2001). Small GTP-Binding Proteins. Physiol. Rev. 81: 153-208 [Abstract] [Full Text]  
• Lippai, M., Tirián, L., Boros, I., Mihály, J., Erdélyi, M., Belecz, I., Máthé, E., Pósfai, J., Nagy, A., Udvardy, A., Paraskeva, E., Görlich, D., Szabad, J. (2000). The Ketel Gene Encodes a Drosophila Homologue of Importin-{beta}. Genetics 156: 1889-1900 [Abstract] [Full Text]  
• Petersen, J. M., Her, L.-S., Varvel, V., Lund, E., Dahlberg, J. E. (2000). The Matrix Protein of Vesicular Stomatitis Virus Inhibits Nucleocytoplasmic Transport When It Is in the Nucleus and Associated with Nuclear Pore Complexes. Mol. Cell. Biol. 20: 8590-8601 [Abstract] [Full Text]  
• Jensen, T. H., Neville, M., Rain, J. C., McCarthy, T., Legrain, P., Rosbash, M. (2000). Identification of Novel Saccharomyces cerevisiae Proteins with Nuclear Export Activity: Cell Cycle-Regulated Transcription Factor Ace2p Shows Cell Cycle-Independent Nucleocytoplasmic Shuttling. Mol. Cell. Biol. 20: 8047-8058 [Abstract] [Full Text]  
• Stage-Zimmermann, T., Schmidt, U., Silver, P. A. (2000). Factors Affecting Nuclear Export of the 60S Ribosomal Subunit In Vivo. Mol. Biol. Cell 11: 3777-3789 [Abstract] [Full Text]  
• Lane, C. M., Cushman, I., Moore, M. S. (2000). Selective Disruption of Nuclear Import by a Functional Mutant Nuclear Transport Carrier. J. Cell Biol. 151: 321-332 [Abstract] [Full Text]  
• Kuang, Y., Garcia-Higuera, I., Moran, A., Mondoux, M., Digweed, M., D'Andrea, A. D. (2000). Carboxy terminal region of the Fanconi anemia protein, FANCG/XRCC9, is required for functional activity. Blood 96: 1625-1632 [Abstract] [Full Text]  
• Peng, G., Hopper, J. E. (2000). Evidence for Gal3p's Cytoplasmic Location and Gal80p's Dual Cytoplasmic-Nuclear Location Implicates New Mechanisms for Controlling Gal4p Activity in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 5140-5148 [Abstract] [Full Text]  
• Ojala, P. M., Sodeik, B., Ebersold, M. W., Kutay, U., Helenius, A. (2000). Herpes Simplex Virus Type 1 Entry into Host Cells: Reconstitution of Capsid Binding and Uncoating at the Nuclear Pore Complex In Vitro. Mol. Cell. Biol. 20: 4922-4931 [Abstract] [Full Text]  
• Sekito, T., Thornton, J., Butow, R. A. (2000). Mitochondria-to-Nuclear Signaling Is Regulated by the Subcellular Localization of the Transcription Factors Rtg1p and Rtg3p. Mol. Biol. Cell 11: 2103-2115 [Abstract] [Full Text]  
• Wente, S. R. (2000). Gatekeepers of the Nucleus. Science 288: 1374-1377 [Abstract] [Full Text]  
• Nemergut, M. E., Macara, I. G. (2000). Nuclear Import of the Ran Exchange Factor, RCC1, Is Mediated by at Least Two Distinct Mechanisms. J. Cell Biol. 149: 835-850 [Abstract] [Full Text]  
• Steggerda, S. M., Black, B. E., Paschal, B. M. (2000). Monoclonal Antibodies to NTF2 Inhibit Nuclear Protein Import by Preventing Nuclear Translocation of the GTPase Ran. Mol. Biol. Cell 11: 703-719 [Abstract] [Full Text]  
• Lau, D., Kunzler, M., Braunwarth, A., Hellmuth, K., Podtelejnikov, A., Mann, M., Hurt, E. (2000). Purification of Protein A-tagged Yeast Ran Reveals Association with a Novel Karyopherin beta Family Member, Pdr6p. J. Biol. Chem. 275: 467-471 [Abstract] [Full Text]  
• Sarkar, S., Azad, A. K., Hopper, A. K. (1999). Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 96: 14366-14371 [Abstract] [Full Text]  
• Welch, K., Franke, J., Kohler, M., Macara, I. G. (1999). RanBP3 Contains an Unusual Nuclear Localization Signal That Is Imported Preferentially by Importin-alpha 3. Mol. Cell. Biol. 19: 8400-8411 [Abstract] [Full Text]  
• Clau{beta}en, M., Rudt, F., Pieler, T. (1999). Functional Modules in Ribosomal Protein L5 for Ribonucleoprotein Complex Formation and Nucleocytoplasmic Transport. J. Biol. Chem. 274: 33951-33958 [Abstract] [Full Text]  
• Alepuz, P. M., Matheos, D., Cunningham, K. W., Estruch, F. (1999). The Saccharomyces cerevisiae RanGTP-Binding Protein Msn5p Is Involved in Different Signal Transduction Pathways. Genetics 153: 1219-1231 [Abstract] [Full Text]  
• Morehouse, H., Buratowski, R. M., Silver, P. A., Buratowski, S. (1999). The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc. Natl. Acad. Sci. USA 96: 12542-12547 [Abstract] [Full Text]  
• Titov, A. A., Blobel, G. (1999). The Karyopherin Kap122p/Pdr6p Imports Both Subunits of the Transcription Factor IIA into the Nucleus. J. Cell Biol. 147: 235-246 [Abstract] [Full Text]  
• Bodendorf, U., Cziepluch, C., Jauniaux, J.-C., Rommelaere, J., Salomé, N. (1999). Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice. J. Virol. 73: 7769-7779 [Abstract] [Full Text]  
• Askjaer, P., Bachi, A., Wilm, M., Bischoff, F. R., Weeks, D. L., Ogniewski, V., Ohno, M., Niehrs, C., Kjems, J., Mattaj, I. W., Fornerod, M. (1999). RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase. Mol. Cell. Biol. 19: 6276-6285 [Abstract] [Full Text]  
• Kudo, N., Matsumori, N., Taoka, H., Fujiwara, D., Schreiner, E. P., Wolff, B., Yoshida, M., Horinouchi, S. (1999). Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc. Natl. Acad. Sci. USA 96: 9112-9117 [Abstract] [Full Text]  
• Takizawa, C. G., Weis, K., Morgan, D. O. (1999). Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta. Proc. Natl. Acad. Sci. USA 96: 7938-7943 [Abstract] [Full Text]  
• Whalen, W. A., Yoon, J. H., Shen, R., Dhar, R. (1999). Regulation of mRNA Export by Nutritional Status in Fission Yeast. Genetics 152: 827-838 [Abstract] [Full Text]  
• Nakashima, N., Noguchi, E., Nishimoto, T. (1999). Saccharomyces cerevisiae Putative G Protein, Gtr1p, Which Forms Complexes With Itself and a Novel Protein Designated as Gtr2p, Negatively Regulates the Ran/Gsp1p G Protein Cycle Through Gtr2p. Genetics 152: 853-867 [Abstract] [Full Text]  
• Novoa, I., Rush, M. G., D'Eustachio, P. (1999). Isolated Mammalian and Schizosaccharomyces pombe Ran-binding Domains Rescue S. pombe sbp1 (RanBP1) Genomic Mutants. Mol. Biol. Cell 10: 2175-2190 [Abstract] [Full Text]  
• Nagoshi, E., Imamoto, N., Sato, R., Yoneda, Y. (1999). Nuclear Import of Sterol Regulatory Element-binding Protein-2, a Basic Helix-Loop-Helix-Leucine Zipper (bHLH-Zip)-containing Transcription Factor, Occurs through the Direct Interaction of Importin beta with HLH-Zip. Mol. Biol. Cell 10: 2221-2233 [Abstract] [Full Text]  
• Pemberton, L. F., Rosenblum, J. S., Blobel, G. (1999). Nuclear Import of the TATA-binding Protein: Mediation by the Karyopherin Kap114p and a Possible Mechanism for Intranuclear Targeting. J. Cell Biol. 145: 1407-1417 [Abstract] [Full Text]  
• Kataoka, N., Bachorik, J. L., Dreyfuss, G. (1999). Transportin-SR, a Nuclear Import Receptor for SR Proteins. J. Cell Biol. 145: 1145-1152 [Abstract] [Full Text]  
• Farjot, G., Sergeant, A., Mikaelian, I. (1999). A New Nucleoporin-like Protein Interacts with Both HIV-1 Rev Nuclear Export Signal and CRM-1. J. Biol. Chem. 274: 17309-17317 [Abstract] [Full Text]  
• Liu, Y., Guo, W., Tartakoff, P. Y., Tartakoff, A. M. (1999). A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p. Proc. Natl. Acad. Sci. USA 96: 6739-6744 [Abstract] [Full Text]  
• Kehlenbach, R. H., Dickmanns, A., Kehlenbach, A., Guan, T., Gerace, L. (1999). A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export. J. Cell Biol. 145: 645-657 [Abstract] [Full Text]  
• Gaits, F., Russell, P. (1999). Active Nucleocytoplasmic Shuttling Required for Function and Regulation of Stress-Activated Kinase Spc1/StyI in Fission Yeast. Mol. Biol. Cell 10: 1395-1407 [Abstract] [Full Text]  
• Paraskeva, E., Izaurralde, E., Bischoff, F. R., Huber, J., Kutay, U., Hartmann, E., Luhrmann, R., Gorlich, D. (1999). CRM1-mediated Recycling of Snurportin 1 to the Cytoplasm. J. Cell Biol. 145: 255-264 [Abstract] [Full Text]  
• Bogerd, H. P., Benson, R. E., Truant, R., Herold, A., Phingbodhipakkiya, M., Cullen, B. R. (1999). Definition of a Consensus Transportin-specific Nucleocytoplasmic Transport Signal. J. Biol. Chem. 274: 9771-9777 [Abstract] [Full Text]  
• Yokoya, F., Imamoto, N., Tachibana, T., Yoneda, Y. (1999). beta -Catenin Can Be Transported into the Nucleus in a Ran-unassisted Manner. Mol. Biol. Cell 10: 1119-1131 [Abstract] [Full Text]  
• Turpin, P., Hay, R. T., Dargemont, C. (1999). Characterization of Ikappa Balpha Nuclear Import Pathway. J. Biol. Chem. 274: 6804-6812 [Abstract] [Full Text]  
• Ullman, K. S., Shah, S., Powers, M. A., Forbes, D. J. (1999). The Nucleoporin Nup153 Plays a Critical Role in Multiple Types of Nuclear Export. Mol. Biol. Cell 10: 649-664 [Abstract] [Full Text]  
• Hieda, M., Tachibana, T., Yokoya, F., Kose, S., Imamoto, N., Yoneda, Y. (1999). A Monoclonal Antibody to the COOH-terminal Acidic Portion of Ran Inhibits Both the Recycling of Ran and Nuclear Protein Import in Living Cells. J. Cell Biol. 144: 645-655 [Abstract] [Full Text]  
• Seedorf, M., Damelin, M., Kahana, J., Taura, T., Silver, P. A. (1999). Interactions between a Nuclear Transporter and a Subset of Nuclear Pore Complex Proteins Depend on Ran GTPase. Mol. Cell. Biol. 19: 1547-1557 [Abstract] [Full Text]  
• Rosorius, O, Reichart, B, Kratzer, F, Heger, P, Dabauvalle, M., Hauber, J (1999). Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1. J. Cell Sci. 112: 2369-2380 [Abstract]  
• Zolotukhin, A. S., Felber, B. K. (1999). Nucleoporins Nup98 and Nup214 Participate in Nuclear Export of Human Immunodeficiency Virus Type 1 Rev. J. Virol. 73: 120-127 [Abstract] [Full Text]  
• Wolin, S. L., Matera, A. G. (1999). The trials and travels of tRNA. Genes Dev. 13: 1-10 [Full Text]  
• Marelli, M., Aitchison, J. D., Wozniak, R. W. (1998). Specific Binding of the Karyopherin Kap121p to a Subunit of the Nuclear Pore Complex Containing Nup53p, Nup59p, and Nup170p. J. Cell Biol. 143: 1813-1830 [Abstract] [Full Text]  
• Hood, J. K., Silver, P. A. (1998). Cse1p Is Required for Export of Srp1p/Importin-alpha from the Nucleus in Saccharomyces cerevisiae. J. Biol. Chem. 273: 35142-35146 [Abstract] [Full Text]  
• Albertini, M., Pemberton, L. F., Rosenblum, J. S., Blobel, G. (1998). A Novel Nuclear Import Pathway for the Transcription Factor TFIIS. J. Cell Biol. 143: 1447-1455 [Abstract] [Full Text]  
• Askjaer, P., Jensen, T. H., Nilsson, J., Englmeier, L., Kjems, J. (1998). The Specificity of the CRM1-Rev Nuclear Export Signal Interaction Is Mediated by RanGTP. J. Biol. Chem. 273: 33414-33422 [Abstract] [Full Text]  
• Jenkins, Y., McEntee, M., Weis, K., Greene, W. C. (1998). Characterization of HIV-1 Vpr Nuclear Import: Analysis of Signals and Pathways. J. Cell Biol. 143: 875-885 [Abstract] [Full Text]  
• Nakamura, M., Masuda, H., Horii, J., Kuma, K.-i., Yokoyama, N., Ohba, T., Nishitani, H., Miyata, T., Tanaka, M., Nishimoto, T. (1998). When Overexpressed, a Novel Centrosomal Protein, RanBPM, Causes Ectopic Microtubule Nucleation Similar to gamma -Tubulin. J. Cell Biol. 143: 1041-1052 [Abstract] [Full Text]  
• Hellmuth, K., Lau, D. M., Bischoff, F. R., Künzler, M., Hurt, E., Simos, G. (1998). Yeast Los1p Has Properties of an Exportin-Like Nucleocytoplasmic Transport Factor for tRNA. Mol. Cell. Biol. 18: 6374-6386 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents