J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/08/707/11 $2.00
Volume 138, Number 3, August 11, 1997 707-717

CD36 Mediates the In Vitro Inhibitory Effects of Thrombospondin-1 on Endothelial Cells

David W. Dawson,^* S. Frieda A. Pearce,^§ Ruiqin Zhong, Roy L. Silverstein,^§ William A. Frazier, and Noël P. Bouck^*

^* Department of Microbiology-Immunology and Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, Illinois 60611;  Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110; and ^§ Department of Medicine, Division of Hematology-Oncology, Cornell University Medical College, New York 10021

Thrombospondin-1 (TSP-1) is a naturally occurring inhibitor of angiogenesis that is able to make normal endothelial cells unresponsive to a wide variety of inducers. Here we use both native TSP-1 and small antiangiogenic peptides derived from it to show that this inhibition is mediated by CD36, a transmembrane glycoprotein found on microvascular endothelial cells. Both IgG antibodies against CD36 and glutathione-S-transferase-CD36 fusion proteins that contain the TSP-1 binding site blocked the ability of intact TSP-1 and its active peptides to inhibit the migration of cultured microvascular endothelial cells. In addition, antiangiogenic TSP-1 peptides inhibited the binding of native TSP-1 to solid phase CD36 and its fusion proteins, as well as to CD36-expressing cells. Additional molecules known to bind CD36, including the IgM anti-CD36 antibody SM, oxidized (but not unoxidized) low density lipoprotein, and human collagen 1, mimicked TSP-1 by inhibiting the migration of human microvascular endothelial cells. Transfection of CD36-deficient human umbilical vein endothelial cells with a CD36 expression plasmid caused them to become sensitive to TSP-1 inhibition of their migration and tube formation. This work demonstrates that endothelial CD36, previously thought to be involved only in adhesion and scavenging activities, may be essential for the inhibition of angiogenesis by thrombospondin-1.

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• Bocci, G., Francia, G., Man, S., Lawler, J., Kerbel, R. S. (2003). Thrombospondin 1, a mediator of the antiangiogenic effects of low-dose metronomic chemotherapy. Proc. Natl. Acad. Sci. USA 100: 12917-12922 [Abstract] [Full Text]  
• Calzada, M. J., Sipes, J. M., Krutzsch, H. C., Yurchenco, P. D., Annis, D. S., Mosher, D. F., Roberts, D. D. (2003). Recognition of the N-terminal Modules of Thrombospondin-1 and Thrombospondin-2 by {alpha}6{beta}1 Integrin. J. Biol. Chem. 278: 40679-40687 [Abstract] [Full Text]  
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• Orr, A. W., Elzie, C. A., Kucik, D. F., Murphy-Ullrich, J. E. (2003). Thrombospondin signaling through the calreticulin/LDL receptor-related protein co-complex stimulates random and directed cell migration. J. Cell Sci. 116: 2917-2927 [Abstract] [Full Text]  
• Miyata, Y., Koga, S., Takehara, K., Kanetake, H., Kanda, S. (2003). Expression of Thrombospondin-derived 4N1K Peptide-containing Proteins in Renal Cell Carcinoma Tissues Is Associated with a Decrease in Tumor Growth and Angiogenesis. Clin. Cancer Res. 9: 1734-1740 [Abstract] [Full Text]  
• Yabe, U., Sato, C., Matsuda, T., Kitajima, K. (2003). Polysialic Acid in Human Milk. CD36 IS A NEW MEMBER OF MAMMALIAN POLYSIALIC ACID-CONTAINING GLYCOPROTEIN. J. Biol. Chem. 278: 13875-13880 [Abstract] [Full Text]  
• Moodley, Y., Rigby, P., Bundell, C., Bunt, S., Hayashi, H., Misso, N., McAnulty, R., Laurent, G., Scaffidi, A., Thompson, P., Knight, D. (2003). Macrophage Recognition and Phagocytosis of Apoptotic Fibroblasts Is Critically Dependent on Fibroblast-Derived Thrombospondin 1 and CD36. Am. J. Pathol. 162: 771-779 [Abstract] [Full Text]  
• Kanda, S., Mochizuki, Y., Kanetake, H. (2003). Stromal Cell-derived Factor-1alpha Induces Tube-like Structure Formation of Endothelial Cells through Phosphoinositide 3-Kinase. J. Biol. Chem. 278: 257-262 [Abstract] [Full Text]  
• Tan, K., Duquette, M., Liu, J.-h., Dong, Y., Zhang, R., Joachimiak, A., Lawler, J., Wang, J.-h. (2002). Crystal structure of the TSP-1 type 1 repeats: a novel layered fold and its biological implication. J. Cell Biol. 159: 373-382 [Abstract] [Full Text]  
• Silverstein, R. L. (2002). The face of TSR revealed: an extracellular signaling domain is exposed. J. Cell Biol. 159: 203-206 [Abstract] [Full Text]  
• Podrez, E. A., Poliakov, E., Shen, Z., Zhang, R., Deng, Y., Sun, M., Finton, P. J., Shan, L., Febbraio, M., Hajjar, D. P., Silverstein, R. L., Hoff, H. F., Salomon, R. G., Hazen, S. L. (2002). A Novel Family of Atherogenic Oxidized Phospholipids Promotes Macrophage Foam Cell Formation via the Scavenger Receptor CD36 and Is Enriched in Atherosclerotic Lesions. J. Biol. Chem. 277: 38517-38523 [Abstract] [Full Text]  
• Kanda, S., Mochizuki, Y., Miyata, Y., Kanetake, H., Yamamoto, N. (2002). Effects of Vitamin D3-Binding Protein-Derived Macrophage Activating Factor (GcMAF) on Angiogenesis. JNCI J Natl Cancer Inst 94: 1311-1319 [Abstract] [Full Text]  
• Ichii, T., Koyama, H., Tanaka, S., Shioi, A., Okuno, Y., Otani, S., Nishizawa, Y. (2002). Thrombospondin-1 Mediates Smooth Muscle Cell Proliferation Induced by Interaction With Human Platelets. Arterioscler. Thromb. Vasc. Bio. 22: 1286-1292 [Abstract] [Full Text]  
• Kincer, J. F., Uittenbogaard, A., Dressman, J., Guerin, T. M., Febbraio, M., Guo, L., Smart, E. J. (2002). Hypercholesterolemia Promotes a CD36-dependent and Endothelial Nitric-oxide Synthase-mediated Vascular Dysfunction. J. Biol. Chem. 277: 23525-23533 [Abstract] [Full Text]  
• Armstrong, L. C., Bjorkblom, B., Hankenson, K. D., Siadak, A. W., Stiles, C. E., Bornstein, P. (2002). Thrombospondin 2 Inhibits Microvascular Endothelial Cell Proliferation by a Caspase-independent Mechanism. Mol. Biol. Cell 13: 1893-1905 [Abstract] [Full Text]  
• Sato, O., Kuriki, C., Fukui, Y., Motojima, K. (2002). Dual Promoter Structure of Mouse and Human Fatty Acid Translocase/CD36 Genes and Unique Transcriptional Activation by Peroxisome Proliferator-activated Receptor alpha and gamma Ligands. J. Biol. Chem. 277: 15703-15711 [Abstract] [Full Text]  
• Bodart, V., Febbraio, M., Demers, A., McNicoll, N., Pohankova, P., Perreault, A., Sejlitz, T., Escher, E., Silverstein, R.L., Lamontagne, D., Ong, H. (2002). CD36 Mediates the Cardiovascular Action of Growth Hormone-Releasing Peptides in the Heart. Circ. Res. 90: 844-849 [Abstract] [Full Text]  
• Lange-Asschenfeldt, B., Weninger, W., Velasco, P., Kyriakides, T. R., von Andrian, U. H., Bornstein, P., Detmar, M. (2002). Increased and prolonged inflammation and angiogenesis in delayed-type hypersensitivity reactions elicited in the skin of thrombospondin-2-deficient mice. Blood 99: 538-545 [Abstract] [Full Text]  
• Gruarin, P., Primo, L., Ferrandi, C., Bussolino, F., Tandon, N. N., Arese, P., Ulliers, D., Alessio, M. (2001). Cytoadherence of Plasmodium falciparum-Infected Erythrocytes Is Mediated by a Redox-Dependent Conformational Fraction of CD36. J. Immunol. 167: 6510-6517 [Abstract] [Full Text]  
• Finnemann, S. C., Silverstein, R. L. (2001). Differential Roles of CD36 and {alpha}v{beta}5 Integrin in Photoreceptor Phagocytosis by the Retinal Pigment Epithelium. J. Exp. Med. 194: 1289-1298 [Abstract] [Full Text]  
• Miao, W.-M., Lin Seng, W., Duquette, M., Lawler, P., Laus, C., Lawler, J. (2001). Thrombospondin-1 Type 1 Repeat Recombinant Proteins Inhibit Tumor Growth through Transforming Growth Factor-{beta}-dependent and -independent Mechanisms. Cancer Res. 61: 7830-7839 [Abstract] [Full Text]  
• Lawler, J., Miao, W.-M., Duquette, M., Bouck, N., Bronson, R. T., Hynes, R. O. (2001). Thrombospondin-1 Gene Expression Affects Survival and Tumor Spectrum of p53-Deficient Mice. Am. J. Pathol. 159: 1949-1956 [Abstract] [Full Text]  
• Fosslien, E. (2001). Molecular Pathology of Cyclooxygenase-2 in Cancer-induced Angiogenesis. Annals of Clinical & Laboratory Science 31: 325-348 [Abstract] [Full Text]  
• Kodama, J., Hashimoto, I., Seki, N., Hongo, A., Yoshinouchi, M., Okuda, H., Kudo, T. (2001). Thrombospondin-1 and -2 Messenger RNA Expression in Invasive Cervical Cancer: Correlation with Angiogenesis and Prognosis. Clin. Cancer Res. 7: 2826-2831 [Abstract] [Full Text]  
• Fischer, J. W, Stoll, M., Hahn, A. W.A, Unger, T. (2001). Differential regulation of thrombospondin-1 and fibronectin by angiotensin II receptor subtypes in cultured endothelial cells. Cardiovasc Res 51: 784-791 [Abstract] [Full Text]  
• Wen, S., Stolarov, J., Myers, M. P., Su, J. D., Wigler, M. H., Tonks, N. K., Durden, D. L. (2001). PTEN controls tumor-induced angiogenesis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.081063798v1 [Abstract] [Full Text]  
• RUSNATI, M., TARABOLETTI, G., URBINATI, C., TULIPANO, G., GIULIANI, R., MOLINARI-TOSATTI, M. P., SENNINO, B., GIACCA, M., TYAGI, M., ALBINI, A., NOONAN, D., GIAVAZZI, R., PRESTA, M. (2000). Thrombospondin-1/HIV-1 Tat protein interaction: modulation of the biological activity of extracellular Tat. FASEB J. 14: 1917-1930 [Abstract] [Full Text]  
• Chandrasekaran, L., He, C.-Z., Al-Barazi, H., Krutzsch, H. C., Iruela-Arispe, M. L., Roberts, D. D. (2000). Cell Contact-dependent Activation of alpha 3beta 1 Integrin Modulates Endothelial Cell Responses to Thrombospondin-1. Mol. Biol. Cell 11: 2885-2900 [Abstract] [Full Text]  
• Yehualaeshet, T., O'Connor, R., Begleiter, A., Murphy-Ullrich, J. E., Silverstein, R., Khalil, N. (2000). A CD36 Synthetic Peptide Inhibits Bleomycin-Induced Pulmonary Inflammation and Connective Tissue Synthesis in the Rat. Am. J. Respir. Cell Mol. Bio. 23: 204-212 [Abstract] [Full Text]  
• Shafiee, A., Penn, J. S., Krutzsch, H. C., Inman, J. K., Roberts, D. D., Blake, D. A. (2000). Inhibition of Retinal Angiogenesis by Peptides Derived from Thrombospondin-1. IOVS 41: 2378-2388 [Abstract] [Full Text]  
• Tenan, M., Fulci, G., Albertoni, M., Diserens, A.-C., Hamou, M.-F., El Atifi-Borel, M., Feige, J.-J., Pepper, M. S., Van Meir, E. G. (2000). Thrombospondin-1 Is Downregulated by Anoxia and Suppresses Tumorigenicity of Human Glioblastoma Cells. J. Exp. Med. 191: 1789-1798 [Abstract] [Full Text]  
• Miyajima–Uchida, H., Hayashi, H., Beppu, R., Kuroki, M., Fukami, M., Arakawa, F., Tomita, Y., Kuroki, M., Oshima, K. (2000). Production and Accumulation of Thrombospondin-1 in Human Retinal Pigment Epithelial Cells. IOVS 41: 561-567 [Abstract] [Full Text]  
• Streit, M., Riccardi, L., Velasco, P., Brown, L. F., Hawighorst, T., Bornstein, P., Detmar, M. (1999). Thrombospondin-2: A potent endogenous inhibitor of tumor growth and angiogenesis. Proc. Natl. Acad. Sci. USA 96: 14888-14893 [Abstract] [Full Text]  
• Hofsteenge, J., Blommers, M., Hess, D., Furmanek, A., Miroshnichenko, O. (1999). The Four Terminal Components of the Complement System Are C-Mannosylated on Multiple Tryptophan Residues. J. Biol. Chem. 274: 32786-32794 [Abstract] [Full Text]  
• Wilson, K. E., Li, Z., Kara, M., Gardner, K. L., Roberts, D. D. (1999). {beta}1 Integrin- and Proteoglycan-Mediated Stimulation of T Lymphoma Cell Adhesion and Mitogen-Activated Protein Kinase Signaling by Thrombospondin-1 and Thrombospondin-1 Peptides. J. Immunol. 163: 3621-3628 [Abstract] [Full Text]  
• Iruela-Arispe, M. L., Lombardo, M., Krutzsch, H. C., Lawler, J., Roberts, D. D. (1999). Inhibition of Angiogenesis by Thrombospondin-1 Is Mediated by 2 Independent Regions Within the Type 1 Repeats. Circulation 100: 1423-1431 [Abstract] [Full Text]  
• Silverstein, R. L., Nachman, R. L. (1999). Angiogenesis and Atherosclerosis : The Mandate Broadens. Circulation 100: 783-785 [Full Text]  
• Krutzsch, H. C., Choe, B. J., Sipes, J. M., Guo, N.-h., Roberts, D. D. (1999). Identification of an alpha 3beta 1 Integrin Recognition Sequence in Thrombospondin-1. J. Biol. Chem. 274: 24080-24086 [Abstract] [Full Text]  
• Sipes, J. M., Krutzsch, H. C., Lawler, J., Roberts, D. D. (1999). Cooperation between Thrombospondin-1 Type 1 Repeat Peptides and alpha vbeta 3 Integrin Ligands to Promote Melanoma Cell Spreading and Focal Adhesion Kinase Phosphorylation. J. Biol. Chem. 274: 22755-22762 [Abstract] [Full Text]  
• Streit, M., Velasco, P., Brown, L. F., Skobe, M., Richard, L., Riccardi, L., Lawler, J., Detmar, M. (1999). Overexpression of Thrombospondin-1 Decreases Angiogenesis and Inhibits the Growth of Human Cutaneous Squamous Cell Carcinomas. Am. J. Pathol. 155: 441-452 [Abstract] [Full Text]  
• Danik, M., Chinn, A. M., Lafeuillade, B., Keramidas, M., Aguesse-Germon, S., Penhoat, A., Chen, H., Mosher, D. F., Chambaz, E. M., Feige, J.-J. (1999). Bovine Thrombospondin-2: Complete Complementary Deoxyribonucleic Acid Sequence and Immunolocalization in the External Zones of the Adrenal Cortex. Endocrinology 140: 2771-2780 [Abstract] [Full Text]  
• Chandrasekaran, S., Guo, N.-h., Rodrigues, R. G., Kaiser, J., Roberts, D. D. (1999). Pro-adhesive and Chemotactic Activities of Thrombospondin-1 for Breast Carcinoma Cells Are Mediated by alpha 3beta 1 Integrin and Regulated by Insulin-like Growth Factor-1 and CD98. J. Biol. Chem. 274: 11408-11416 [Abstract] [Full Text]  
• Bleuel, K., Popp, S., Fusenig, N. E., Stanbridge, E. J., Boukamp, P. (1999). Tumor suppression in human skin carcinoma cells by chromosome 15 transfer or thrombospondin-1 overexpression through halted tumor vascularization. Proc. Natl. Acad. Sci. USA 96: 2065-2070 [Abstract] [Full Text]  
• Pellerin, S., Croizet, K., Rabilloud, R., Feige, J.-J., Rousset, B. (1999). Regulation of the Three-Dimensional Organization of Thyroid Epithelial Cells into Follicle Structures by the Matricellular Protein, Thrombospondin-1. Endocrinology 140: 1094-1103 [Abstract] [Full Text]  
• Dawson, D. W., Volpert, O. V., Pearce, S. F. A., Schneider, A. J., Silverstein, R. L., Henkin, J., Bouck, N. P. (1999). Three Distinct D-Amino Acid Substitutions Confer Potent Antiangiogenic Activity on an Inactive Peptide Derived from a Thrombospondin-1 Type 1 Repeat. Mol. Pharmacol. 55: 332-338 [Abstract] [Full Text]  
• Suzuma, K., Takagi, H., Otani, A., Oh, H., Honda, Y. (1999). Expression of Thrombospondin-1 in Ischemia-Induced Retinal Neovascularization. Am. J. Pathol. 154: 343-354 [Abstract] [Full Text]  
• Kraling, B., Wiederschain, D., Boehm, T, Rehn, M, Mulliken, J., Moses, M. (1999). The role of matrix metalloproteinase activity in the maturation of human capillary endothelial cells in vitro. J. Cell Sci. 112: 1599-1609 [Abstract]  
• Volpert, O. V., Fong, T., Koch, A. E., Peterson, J. D., Waltenbaugh, C., Tepper, R. I., Bouck, N. P. (1998). Inhibition of Angiogenesis by Interleukin 4 . J. Exp. Med. 188: 1039-1046 [Abstract] [Full Text]  
• Sheibani, N., Frazier, W. A. (1998). Down-Regulation of Platelet Endothelial Cell Adhesion Molecule-1 Results in Thrombospondin-1 Expression and Concerted Regulation of Endothelial Cell Phenotype. Mol. Biol. Cell 9: 701-713 [Abstract] [Full Text]  
• Wang, X.-Q., Frazier, W. A. (1998). The Thrombospondin Receptor CD47 (IAP) Modulates and Associates with alpha 2beta 1 Integrin in Vascular Smooth Muscle Cells. Mol. Biol. Cell 9: 865-874 [Abstract] [Full Text]  
• Crombie, R., Silverstein, R. (1998). Lysosomal Integral Membrane Protein II Binds Thrombospondin-1. STRUCTURE-FUNCTION HOMOLOGY WITH THE CELL ADHESION MOLECULE CD36 DEFINES A CONSERVED RECOGNITION MOTIF. J. Biol. Chem. 273: 4855-4863 [Abstract] [Full Text]  
• Hartmann, S., Hofsteenge, J. (2000). Properdin, the Positive Regulator of Complement, Is Highly C-Mannosylated. J. Biol. Chem. 275: 28569-28574 [Abstract] [Full Text]  
• Bein, K., Simons, M. (2000). Thrombospondin Type 1 Repeats Interact with Matrix Metalloproteinase 2. REGULATION OF METALLOPROTEINASE ACTIVITY. J. Biol. Chem. 275: 32167-32173 [Abstract] [Full Text]  
• Thorne, R. F., Marshall, J. F., Shafren, D. R., Gibson, P. G., Hart, I. R., Burns, G. F. (2000). The Integrins alpha 3beta 1 and alpha 6beta 1 Physically and Functionally Associate with CD36 in Human Melanoma Cells. REQUIREMENT FOR THE EXTRACELLULAR DOMAIN OF CD36. J. Biol. Chem. 275: 35264-35275 [Abstract] [Full Text]  
• Miyahara, T., Schrum, L., Rippe, R., Xiong, S., Yee, H. F. Jr., Motomura, K., Anania, F. A., Willson, T. M., Tsukamoto, H. (2000). Peroxisome Proliferator-activated Receptors and Hepatic Stellate Cell Activation. J. Biol. Chem. 275: 35715-35722 [Abstract] [Full Text]  

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