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Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
Previously we isolated tub2-423, a cold-sensitive allele of the Saccharomyces cerevisiae gene encoding 
-tubulin that confers a defect in mitotic spindle
function. In an attempt to identify additional proteins
that are important for spindle function, we screened for
suppressors of the cold sensitivity of tub2-423 and obtained two alleles of a novel gene, STU2. STU2 is an essential gene and encodes a protein whose sequence is
similar to proteins identified in a variety of organisms.
Stu2p localizes primarily to the spindle pole body
(SPB) and to a lesser extent along spindle microtubules. Localization to the SPB is not dependent on the presence of microtubules, indicating that Stu2p is an integral component of the SPB. Stu2p also binds microtubules in vitro. We have localized the microtubule-binding domain of Stu2p to a highly basic 100-amino acid
region. This region contains two imperfect repeats;
both repeats appear to contribute to microtubule binding to similar extents. These results suggest that Stu2p
may play a role in the attachment, organization, and/or
dynamics of microtubule ends at the SPB.
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