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J. Cell Biol.,
Volume 140, Number 1, January 12, 1998 101-110
Department of Molecular Biology and Department of Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211,
Geneva 4, Switzerland
Chloroplast subfractions were tested with a
UV cross-linking assay for proteins that bind to the 5
untranslated region of the chloroplast psbC mRNA of
the green alga Chlamydomonas reinhardtii. These analyses revealed that RNA-binding proteins of 30-32, 46, 47, 60, and 80 kD are associated with chloroplast membranes. The buoyant density and the acyl lipid composition of these membranes are compatible with their origin being the inner chloroplast envelope membrane.
However, unlike previously characterized inner envelope membranes, these membranes are associated with
thylakoids. One of the membrane-associated RNA-binding proteins appears to be RB47, which has been
reported to be a specific activator of psbA mRNA
translation. These results suggest that translation of
chloroplast mRNAs encoding thylakoid proteins occurs
at either a subfraction of the chloroplast inner envelope
membrane or a previously uncharacterized intra-chloroplast compartment, which is physically associated with thylakoids.
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