J. Cell Biol., Volume 140, Number 2, January 26, 1998 325-334

Capacitative Ca²⁺ Entry Is Closely Linked to the Filling State of Internal Ca²⁺ Stores: A Study Using Simultaneous Measurements of I_CRAC and Intraluminal [Ca²⁺]

Aldebaran M. Hofer, Cristina Fasolato, and Tullio Pozzan

University of Padova, Department of Biomedical Sciences, CNR Center for Biomembranes, I-35121 Padova, Italy

I_CRAC (the best characterized Ca²⁺ current activated by store depletion) was monitored concurrently for the first time with [Ca²⁺] changes in internal stores. To establish the quantitative and kinetic relationship between these two parameters, we have developed a novel means to clamp [Ca²⁺] within stores of intact cells at any level. The advantage of this approach, which is based on the membrane-permeant low-affinity Ca²⁺ chelator N,N,N,N-tetrakis (2-pyridylmethyl)ethylene diamine (TPEN), is that [Ca²⁺] within the ER can be lowered and restored to its original level within 10-15 s without modifications of Ca²⁺ pumps or release channels. Using these new tools, we demonstrate here that Ca²⁺ release-activated Ca²⁺ current (I_CRAC) is activated (a) solely by reduction of free [Ca²⁺] within the ER and (b) by any measurable decrease in [Ca²⁺]_ER. We also demonstrate that the intrinsic kinetics of inactivation are relatively slow and possibly dependent on soluble factors that are lost during the whole-cell recording.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Walsh, B. M., Naik, H. B., Dubach, J. M., Beshire, M., Wieland, A. M., Soybel, D. I. (2007). Thiol-oxidant monochloramine mobilizes intracellular Ca2+ in parietal cells of rabbit gastric glands. Am. J. Physiol. Cell Physiol. 293: C1687-C1697 [Abstract] [Full Text]  
• Chung, S. C., Limnander, A., Kurosaki, T., Weiss, A., Korenbrot, J. I. (2007). Coupling Ca2+ store release to Icrac channel activation in B lymphocytes requires the activity of Lyn and Syk kinases. J. Cell Biol. 177: 317-328 [Abstract] [Full Text]  
• Csutora, P., Zarayskiy, V., Peter, K., Monje, F., Smani, T., Zakharov, S. I., Litvinov, D., Bolotina, V. M. (2006). Activation Mechanism for CRAC Current and Store-operated Ca2+ Entry: CALCIUM INFLUX FACTOR AND Ca2+-INDEPENDENT PHOSPHOLIPASE A2beta-MEDIATED PATHWAY. J. Biol. Chem. 281: 34926-34935 [Abstract] [Full Text]  
• Ma, R., Du, J., Sours, S., Ding, M. (2006). Store-Operated Ca2+ Channel in Renal Microcirculation and Glomeruli. Exp. Biol. Med. 231: 145-153 [Abstract] [Full Text]  
• Cima, R. R., Dubach, J. M., Wieland, A. M., Walsh, B. M., Soybel, D. I. (2006). Intracellular Ca2+ and Zn2+ signals during monochloramine-induced oxidative stress in isolated rat colon crypts. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G250-G261 [Abstract] [Full Text]  
• Kusters, J. M. A. M., Dernison, M. M., van Meerwijk, W. P. M., Ypey, D. L., Theuvenet, A. P. R., Gielen, C. C. A. M. (2005). Stabilizing Role of Calcium Store-Dependent Plasma Membrane Calcium Channels in Action-Potential Firing and Intracellular Calcium Oscillations. Biophys. J 89: 3741-3756 [Abstract] [Full Text]  
• Hooper, K. M., Boletta, A., Germino, G. G., Hu, Q., Ziegelstein, R. C., Sutters, M. (2005). Expression of polycystin-1 enhances endoplasmic reticulum calcium uptake and decreases capacitative calcium entry in ATP-stimulated MDCK cells. Am. J. Physiol. Renal Physiol. 289: F521-F530 [Abstract] [Full Text]  
• Shmist, Y. A., Kamburg, R., Ophir, G., Kozak, A., Shneyvays, V., Appelbaum, Y. J., Shainberg, A. (2005). N,N,N',N'-Tetrakis(2-pyridylmethyl)-ethylenediamine Improves Myocardial Protection against Ischemia by Modulation of Intracellular Ca2+ Homeostasis. J. Pharmacol. Exp. Ther. 313: 1046-1057 [Abstract] [Full Text]  
• Golovina, V. A. (2005). Visualization of localized store-operated calcium entry in mouse astrocytes. Close proximity to the endoplasmic reticulum. J. Physiol. 564: 737-749 [Abstract] [Full Text]  
• Parekh, A. B., Putney, J. W. Jr. (2005). Store-Operated Calcium Channels. Physiol. Rev. 85: 757-810 [Abstract] [Full Text]  
• Malli, R., Frieden, M., Trenker, M., Graier, W. F. (2005). The Role of Mitochondria for Ca2+ Refilling of the Endoplasmic Reticulum. J. Biol. Chem. 280: 12114-12122 [Abstract] [Full Text]  
• Shuttleworth, T. J., Thompson, J. L., Mignen, O. (2004). ARC Channels: A Novel Pathway for Receptor-Activated Calcium Entry. Physiology 19: 355-361 [Abstract] [Full Text]  
• Thorogate, R., Torok, K. (2004). Ca2+-dependent and -independent mechanisms of calmodulin nuclear translocation. J. Cell Sci. 117: 5923-5936 [Abstract] [Full Text]  
• Ma, R., Rundle, D., Jacks, J., Koch, M., Downs, T., Tsiokas, L. (2003). Inhibitor of Myogenic Family, a Novel Suppressor of Store-operated Currents through an Interaction with TRPC1. J. Biol. Chem. 278: 52763-52772 [Abstract] [Full Text]  
• Narayanan, B., Islam, M. N., Bartelt, D., Ochs, R. S. (2003). A Direct Mass-action Mechanism Explains Capacitative Calcium Entry in Jurkat and Skeletal L6 Muscle Cells. J. Biol. Chem. 278: 44188-44196 [Abstract] [Full Text]  
• Caroppo, R., Colella, M., Colasuonno, A., DeLuisi, A., Debellis, L., Curci, S., Hofer, A. M. (2003). A Reassessment of the Effects of Luminal [Ca2+] on Inositol 1,4,5-Trisphosphate-induced Ca2+ Release from Internal Stores. J. Biol. Chem. 278: 39503-39508 [Abstract] [Full Text]  
• Tribe, R. M., Moriarty, P., Dalrymple, A., Hassoni, A. A., Poston, L. (2003). Interleukin-1{beta} Induces Calcium Transients and Enhances Basal and Store Operated Calcium Entry in Human Myometrial Smooth Muscle. Biol. Reprod. 68: 1842-1849 [Abstract] [Full Text]  
• Langston, H. P., Ke, Y., Gewirtz, A. T., Dombrowski, K. E., Kapp, J. A. (2003). Secretion of IL-2 and IFN-{gamma}, But Not IL-4, by Antigen-Specific T Cells Requires Extracellular ATP. J. Immunol. 170: 2962-2970 [Abstract] [Full Text]  
• Baran, I. (2003). Integrated Luminal and Cytosolic Aspects of the Calcium Release Control. Biophys. J 84: 1470-1485 [Abstract] [Full Text]  
• Golovina, V. A., Song, H., James, P. F., Lingrel, J. B., Blaustein, M. P. (2003). Na+ pump alpha 2-subunit expression modulates Ca2+ signaling. Am. J. Physiol. Cell Physiol. 284: C475-C486 [Abstract] [Full Text]  
• Zhang, B.-X., Ma, X., Yeh, C.-K., Lifschitz, M. D., Zhu, M. X., Katz, M. S. (2002). Epidermal Growth Factor-induced Depletion of the Intracellular Ca2+ Store Fails to Activate Capacitative Ca2+ Entry in a Human Salivary Cell Line. J. Biol. Chem. 277: 48165-48171 [Abstract] [Full Text]  
• Zhang, J., Wier, W. G., Blaustein, M. P. (2002). Mg2+ blocks myogenic tone but not K+-induced constriction: role for SOCs in small arteries. Am. J. Physiol. Heart Circ. Physiol. 283: H2692-H2705 [Abstract] [Full Text]  
• Arnaudeau, S., Frieden, M., Nakamura, K., Castelbou, C., Michalak, M., Demaurex, N. (2002). Calreticulin Differentially Modulates Calcium Uptake and Release in the Endoplasmic Reticulum and Mitochondria. J. Biol. Chem. 277: 46696-46705 [Abstract] [Full Text]  
• Vaca, L., Sampieri, A. (2002). Calmodulin Modulates the Delay Period between Release of Calcium from Internal Stores and Activation of Calcium Influx via Endogenous TRP1 Channels. J. Biol. Chem. 277: 42178-42187 [Abstract] [Full Text]  
• Brini, M., Manni, S., Carafoli, E. (2002). Recombinant Expression of the Plasma Membrane Na+/Ca2+ Exchanger Affects Local and Global Ca2+ Homeostasis in Chinese Hamster Ovary Cells. J. Biol. Chem. 277: 38693-38699 [Abstract] [Full Text]  
• Provencher, S. J., Gascon-Barre, M. (2002). Effect of Cyclosporine A on Hepatic Compensatory Growth: Role of Calcium Status. J. Pharmacol. Exp. Ther. 303: 58-65 [Abstract] [Full Text]  
• POWIS, D. A., ZERBES, M. (2002). In Situ Chelation of Ca2+ in Intracellular Stores Induces Capacitative Ca2+ Entry in Bovine Adrenal Chromaffin Cells. Ann. N. Y. Acad. Sci. 971: 150-152 [Abstract] [Full Text]  
• Morita, K., Sakakibara, A., Kitayama, S., Kumagai, K., Tanne, K., Dohi, T. (2002). Pituitary Adenylate Cyclase-Activating Polypeptide Induces a Sustained Increase in Intracellular Free Ca2+ Concentration and Catecholamine Release by Activating Ca2+ Influx via Receptor-Stimulated Ca2+ Entry, Independent of Store-Operated Ca2+ Channels, and Voltage-Dependent Ca2+ Channels in Bovine Adrenal Medullary Chromaffin Cells. J. Pharmacol. Exp. Ther. 302: 972-982 [Abstract] [Full Text]  
• Cronin, S. R. (2002). I'll Simply Die Without My Calcium: Ca2+ Signaling and Surviving Cellular Stress. Mol. Interv. 2: 284-285 [Abstract] [Full Text]  
• Islam, M. N., Narayanan, B., Ochs, R. S. (2002). A Mechanism for Both Capacitative Ca2+ Entry and Excitation-Contraction Coupled Ca2+ Release by the Sarcoplasmic Reticulum of Skeletal Muscle Cells. Exp. Biol. Med. 227: 425-431 [Abstract] [Full Text]  
• Bennett, B. D., Alvarez, U., Hruska, K. A. (2001). Receptor-Operated Osteoclast Calcium Sensing. Endocrinology 142: 1968-1974 [Abstract] [Full Text]  
• Inglefield, J. R., Mundy, W. R., Shafer, T. J. (2001). Inositol 1,4,5-Triphosphate Receptor-Sensitive Ca2+ Release, Store-Operated Ca2+ Entry, and cAMP Responsive Element Binding Protein Phosphorylation in Developing Cortical Cells following Exposure to Polychlorinated Biphenyls. J. Pharmacol. Exp. Ther. 297: 762-773 [Abstract] [Full Text]  
• Wingertzahn, M. A., Ochs, R. S. (2001). Changes in Ryanodine Receptor-Mediated Calcium Release During Skeletal Muscle Differentiation. II. Resolution of a Caffeine -Ryanodine Paradox. Exp. Biol. Med. 226: 119-126 [Abstract] [Full Text]  
• Dyachok, O., Gylfe, E. (2001). Store-operated influx of Ca2+ in pancreatic {beta}-cells exhibits graded dependence on the filling of the endoplasmic reticulum. J. Cell Sci. 114: 2179-2186 [Abstract] [Full Text]  
• Stepien, O., Marche, P. (2000). Amlodipine inhibits thapsigargin-sensitive CA2+ stores in thrombin-stimulated vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 279: H1220-H1227 [Abstract] [Full Text]  
• Garcia-Sancho, J. (2000). SOC and unSOC. Physiology 15: 159-160 [Full Text]  
• Pinton, P., Ferrari, D., Magalhaes, P., Schulze-Osthoff, K., Di Virgilio, F., Pozzan, T., Rizzuto, R. (2000). Reduced Loading of Intracellular Ca2+ Stores and Downregulation of Capacitative Ca2+ Influx In Bcl-2-Overexpressing Cells. J. Cell Biol. 148: 857-862 [Abstract] [Full Text]  
• Mailhot, G., Petit, J.-L., Demers, C., Gascon-Barre, M. (2000). Influence of the in Vivo Calcium Status on Cellular Calcium Homeostasis and the Level of the Calcium-Binding Protein Calreticulin in Rat Hepatocytes. Endocrinology 141: 891-900 [Abstract] [Full Text]  
• Tengholm, A., Hellman, B., Gylfe, E. (1999). Glucose Regulation of Free Ca2+ in the Endoplasmic Reticulum of Mouse Pancreatic Beta Cells. J. Biol. Chem. 274: 36883-36890 [Abstract] [Full Text]  
• Krause, E., Schmid, A., Gonzalez, A., Schulz, I. (1999). Low Cytoplasmic [Ca2+] Activates ICRAC Independently of Global Ca2+ Store Depletion in RBL-1 Cells. J. Biol. Chem. 274: 36957-36962 [Abstract] [Full Text]  
• Broad, L. M., Armstrong, D. L., Putney, J. W. Jr. (1999). Role of the Inositol 1,4,5-Trisphosphate Receptor in Ca2+ Feedback Inhibition of Calcium Release-activated Calcium Current (Icrac). J. Biol. Chem. 274: 32881-32888 [Abstract] [Full Text]  
• Gilon, P., Arredouani, A., Gailly, P., Gromada, J., Henquin, J.-C. (1999). Uptake and Release of Ca2+ by the Endoplasmic Reticulum Contribute to the Oscillations of the Cytosolic Ca2+ Concentration Triggered by Ca2+ Influx in the Electrically Excitable Pancreatic B-cell. J. Biol. Chem. 274: 20197-20205 [Abstract] [Full Text]  
• Fomina, A. F., Nowycky, M. C. (1999). A Current Activated on Depletion of Intracellular Ca2+ Stores Can Regulate Exocytosis in Adrenal Chromaffin Cells. J. Neurosci. 19: 3711-3722 [Abstract] [Full Text]  
• Lin, P., Yao, Y., Hofmeister, R., Tsien, R. Y., Farquhar, M. G. (1999). Overexpression of CALNUC (Nucleobindin) Increases Agonist and Thapsigargin Releasable Ca2+ Storage in the Golgi. J. Cell Biol. 145: 279-289 [Abstract] [Full Text]  
• Hashimoto, A., Hirose, K., Okada, H., Kurosaki, T., Iino, M. (1999). Inhibitory Modulation of B Cell Receptor-mediated Ca2+ Mobilization by Src Homology 2 Domain-containing Inositol 5'-Phosphatase (SHIP). J. Biol. Chem. 274: 11203-11208 [Abstract] [Full Text]  
• Jayadev, S., Petranka, J. G., Cheran, S. K., Biermann, J. A., Barrett, J. C., Murphy, E. (1999). Reduced Capacitative Calcium Entry Correlates with Vesicle Accumulation and Apoptosis. J. Biol. Chem. 274: 8261-8268 [Abstract] [Full Text]  
• Rys-Sikora, K. E., Gill, D. L. (1998). Fatty Acid-mediated Calcium Sequestration within Intracellular Calcium Pools. J. Biol. Chem. 273: 32627-32635 [Abstract] [Full Text]  
• PETTIT, E. J. (1998). Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis. Physiol. Rev. 78: 949-967 [Abstract] [Full Text]  
• Fasolato, C., Pizzo, P., Pozzan, T. (1998). Delayed Activation of the Store-operated Calcium Current Induced by Calreticulin Overexpression in RBL-1 Cells. Mol. Biol. Cell 9: 1513-1522 [Abstract] [Full Text]  
• Arnaudeau, S., Kelley, W. L., Walsh, J. V. Jr., Demaurex, N. (2001). Mitochondria Recycle Ca2+ to the Endoplasmic Reticulum and Prevent the Depletion of Neighboring Endoplasmic Reticulum Regions. J. Biol. Chem. 276: 29430-29439 [Abstract] [Full Text]  
• Liu, X., Ambudkar, I. S. (2001). Characteristics of a Store-operated Calcium-permeable Channel. SARCOENDOPLASMIC RETICULUM CALCIUM PUMP FUNCTION CONTROLS CHANNEL GATING. J. Biol. Chem. 276: 29891-29898 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents