Identification of the t Complex-encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive

doi:10.1083/jcb.140.5.1137

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J. Cell Biol., Volume 140, Number 5, March 9, 1998 1137-1147

Identification of the t Complex-encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive

Alistair Harrison,^* Patricia Olds-Clarke, and Stephen M. King^*

^* Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032-3305, and Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

The cytoplasmic dynein light chain Tctex1 is a candidate for one of the distorter products involved in the non-Mendelian transmissionof mouse t haplotypes. It has been unclear, however, how the t-specificmutations in this protein, which is found associated with cytoplasmicdynein in many tissues, could result in a male germ cell-specificphenotype. Here, we demonstrate that Tctex1 is not only a cytoplasmicdynein component, but is also present both in mouse sperm andChlamydomonas flagella. Genetic and biochemical dissection ofthe Chlamydomonas flagellum reveal that Tctex1 is a previouslyundescribed component of inner dynein arm I1. Combined with therecent identification of another putative t complex distorter,Tctex2, within the outer dynein arm, these results support thehypothesis that transmission ratio distortion (meiotic drive)of mouse t haplotypes involves dysfunction of both flagellar innerand outer dynein arms but does not require the cytoplasmic isozyme.

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