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J. Cell Biol.,
Volume 141, Number 2, April 20, 1998 321-333
§
* Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196; Assembly of the higher-order structure of
mitotic chromosomes is a prerequisite for proper chromosome condensation, segregation and integrity. Understanding the details of this process has been limited
because very few proteins involved in the assembly of chromosome structure have been discovered. Using a
human autoimmune scleroderma serum that identifies
a chromosomal protein in human cells and Drosophila
embryos, we cloned the corresponding Drosophila gene
that encodes the homologue of vertebrate titin based
on protein size, sequence similarity, developmental expression and subcellular localization. Titin is a giant
sarcomeric protein responsible for the elasticity of striated muscle that may also function as a molecular scaffold for myofibrillar assembly. Molecular analysis and
immunostaining with antibodies to multiple titin epitopes indicates that the chromosomal and muscle forms
of titin may vary in their NH2 termini. The identification of titin as a chromosomal component provides a
molecular basis for chromosome structure and elasticity.
Laboratório de Genética Molecular, Instituto de Biologia Molecular e Celular, 4150 Porto, Portugal; and § Departamento de
Biologia Molecular, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050 Porto, Portugal
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