A Role for NIMA in the Nuclear Localization of Cyclin B in Aspergillus nidulans

doi:10.1083/jcb.141.7.1575

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J. Cell Biol., Volume 141, Number 7, June 29, 1998 1575-1587

A Role for NIMA in the Nuclear Localization of Cyclin B in Aspergillus nidulans

L. Wu,^* S.A. Osmani,^* and P.M. Mirabito

^* Henry Hood Research Program, Weis Center for Research, Pennsylvania State University College of Medicine, Danville, Pennsylvania 17822-2617; and Molecular and Cellular Biology Section, School of Biological Sciences, University of Kentucky, Lexington, Kentucky 40506-0225

NIMA promotes entry into mitosis in late G2 by some mechanism that is after activation of the Aspergillus nidulans G2 cyclin-dependentkinase, NIMX^CDC2/NIME^Cyclin ^B. Here we present two independent lines of evidence which indicatethat this mechanism involves control of NIMX^CDC2/NIME^Cyclin ^B localization. First, we found that NIME^Cyclin ^B localized to the nucleus and the nucleus-associated organelle,the spindle pole body, in a NIMA-dependent manner. Analysis ofcells from asynchronous cultures, synchronous cultures, and culturesarrested in S or G2 showed that NIME^Cyclin ^B was predominantly nuclear during interphase, with maximal nuclearaccumulation in late G2. NIMX^CDC2 colocalized with NIME^Cyclin ^B in G2 cells. Although inactivation of NIMA using either the nimA1or nimA5 temperature-sensitive mutations blocked cells in G2,NIMX^CDC2/NIME^Cyclin ^B localization was predominantly cytoplasmic rather than nuclear.Second, we found that nimA interacts genetically with sonA, whichis a homologue of the yeast nucleocytoplasmic transporter GLE2/RAE1.Mutations in sonA were identified as allele-specific suppressorsof nimA1. The sonA1 suppressor alleviated the nuclear divisionand NIME^Cyclin ^B localization defects of nimA1 cells without markedly increasingNIMX^CDC2 or NIMA kinase activity. These results indicate that NIMA promotesthe nuclear localization of the NIMX^CDC2/ NIME^Cyclin ^B complex, by a process involving SONA. This mechanism may be involvedin coordinating the functions of NIMX^CDC2 and NIMA in the regulation of mitosis.

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