J. Cell Biol., Volume 142, Number 2, July 27, 1998 435-442

Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47

Christopher B. Yohn,^* Amybeth Cohen, Cristen Rosch,^§ Michael R. Kuchka,^§ and Stephen P. Mayfield^*

^* Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California 92037;  Department of Biological Science, The California State University, Fullerton, California 92834-6850; and ^§ Department of Biological Science, Lehigh University, Bethlehem, Pennsylvania 18105

A set of nuclear mutants of C. reinhardtii were identified that specifically lack translation of the chloroplast-encoded psbA mRNA, which encodes the photosystem II reaction center polypeptide D1. Two of these mutants are deficient in the 47-kD member (RB47) of the psbA RNA-binding complex, which has previously been identified both genetically and biochemically as a putative translational activator of the chloroplast psbA mRNA. RB47 is a member of the poly(A)-binding protein family, and binds with high affinity and specificity to the 5' untranslated region of the psbA mRNA. The results presented here confirm RB47's role as a message-specific translational activator in the chloroplast, and bring together genetic and biochemical data to form a cohesive model for light- activated translational regulation in the chloroplast.

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