Mitotic Centromere-associated Kinesin Is Important for Anaphase Chromosome Segregation

doi:10.1083/jcb.142.3.787

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J. Cell Biol., Volume 142, Number 3, August 10, 1998 787-801

Mitotic Centromere-associated Kinesin Is Important for Anaphase Chromosome Segregation

Todd Maney, Andrew W. Hunter, Mike Wagenbach, and Linda Wordeman

Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195

Mitotic centromere-associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associateduntil after telophase. MCAK is a homodimer that is encoded bya single gene and has no associated subunits. A motorless versionof MCAK that binds centromeres but not microtubules disrupts chromosomesegregation during anaphase. Antisense-induced depletion of MCAKresults in the same defect. MCAK overexpression induces centromere-independentbundling and eventual loss of spindle microtubule polymer suggestingthat centromere-associated bundling and/or depolymerization activityis required for anaphase. Live cell imaging indicates that MCAKmay be required to coordinate the onset of sister centromere separation.

Key words: MCAK; centromere; kinesin; mitosis; anaphase

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Topper, L. M., Bastians, H., Ruderman, J. V., Gorbsky, G. J. (2001). Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes. J. Cell Biol. 154: 707-718 [Abstract] [Full Text]
Tusher, V. G., Tibshirani, R., Chu, G. (2001). Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. USA 10.1073/pnas.091062498v1 [Abstract] [Full Text]
Gordon, M. B., Howard, L., Compton, D. A. (2001). Chromosome Movement in Mitosis Requires Microtubule Anchorage at Spindle Poles. J. Cell Biol. 152: 425-434 [Abstract] [Full Text]
Ginkel, L. M., Wordeman, L. (2000). Expression and Partial Characterization of Kinesin-related Proteins in Differentiating and Adult Skeletal Muscle. Mol. Biol. Cell 11: 4143-4158 [Abstract] [Full Text]
Sugata, N., Li, S., Earnshaw, W. C., Yen, T. J., Yoda, K., Masumoto, H., Munekata, E., Warburton, P. E., Todokoro, K. (2000). Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes. Hum Mol Genet 9: 2919-2926 [Abstract] [Full Text]
Kim, A., Endow, S. (2000). A kinesin family tree. J. Cell Sci. 113: 3681-3682 [Full Text]
Yucel, J. K., Marszalek, J. D., McIntosh, J. R., Goldstein, L. S.B., Cleveland, D. W., Philp, A. V. (2000). CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila. J. Cell Biol. 150: 1-12 [Abstract] [Full Text]
Ly, D. H., Lockhart, D. J., Lerner, R. A., Schultz, P. G. (2000). Mitotic Misregulation and Human Aging. Science 287: 2486-2492 [Abstract] [Full Text]
Saffery, R., Irvine, D. V., Griffiths, B., Kalitsis, P., Wordeman, L., Choo, K.H. A. (2000). Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins.. Hum Mol Genet 9: 175-185 [Abstract] [Full Text]
Hunter, A., Wordeman, L (2000). How motor proteins influence microtubule polymerization dynamics. J. Cell Sci. 113: 4379-4389 [Abstract]
Starr, D., Saffery, R, Li, Z, Simpson, A., Choo, K., Yen, T., Goldberg, M. (2000). HZwint-1, a novel human kinetochore component that interacts with HZW10. J. Cell Sci. 113: 1939-1950 [Abstract]
Rieder, C., Cole, R (1999). Chromatid cohesion during mitosis: lessons from meiosis. J. Cell Sci. 112: 2607-2613 [Abstract]
Maney, T., Wagenbach, M., Wordeman, L. (2001). Molecular Dissection of the Microtubule Depolymerizing Activity of Mitotic Centromere-associated Kinesin. J. Biol. Chem. 276: 34753-34758 [Abstract] [Full Text]
Tusher, V. G., Tibshirani, R., Chu, G. (2001). Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. USA 98: 5116-5121 [Abstract] [Full Text]