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J. Cell Biol.,
Volume 143, Number 3, November 2, 1998 737-749
* Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94143; The septins are a family of proteins required
for cytokinesis in a number of eukaryotic cell types. In
budding yeast, these proteins are thought to be the
structural components of a filament system present at
the mother-bud neck, called the neck filaments. In this
study, we report the isolation of a protein complex containing the yeast septins Cdc3p, Cdc10p, Cdc11p, and
Cdc12p that is capable of forming long filaments in
vitro. To investigate the relationship between these filaments and the neck filaments, we purified septin complexes from cells deleted for CDC10 or CDC11. These
complexes were not capable of the polymerization exhibited by wild-type preparations, and analysis of the
neck region by electron microscopy revealed that the
cdc10 Department of Biology, Univeristy of North Carolina, Chapel Hill, North Carolina 27599; § Protein and Peptide Group,
European Molecular Biological Laboratory, D-69012 Heidelberg, Germany; and Department of Cell Biology, Harvard Medical
School, Boston, Massachusetts 02115
and cdc11 cells did not contain detectable neck filaments. These results strengthen the hypothesis
that the septins are the major structural components of
the neck filaments. Surprisingly, we found that septin
dependent processes like cytokinesis and the localization of Bud4p to the neck still occurred in cdc10 cells.
This suggests that the septins may be able to function in
the absence of normal polymerization and the formation of a higher order filament structure.
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