Impaired Integrin-mediated Adhesion and Signaling in Fibroblasts Expressing a Dominant-negative Mutant PTP1B

doi:10.1083/jcb.143.3.861

Epitomics: The Rabbit Monoclonal Company

This Article

	Full Text
	PDF (Full Text)
	Addition or Correction (v143,p1761)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Arregui, C. O.
	Articles by Lilien, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Arregui, C. O.
	Articles by Lilien, J.


Social Bookmarking

	What's this?

J. Cell Biol., Volume 143, Number 3, November 2, 1998 861-873

Impaired Integrin-mediated Adhesion and Signaling in Fibroblasts Expressing a Dominant-negative Mutant PTP1B

Carlos O. Arregui, Janne Balsamo, and Jack Lilien

Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202

To investigate the role of nonreceptor protein tyrosine phosphatase 1B (PTP1B) in $beta$ 1-integrin- mediated adhesion and signaling,we transfected mouse L cells with normal and catalytically inactiveforms of the phosphatase. Parental cells and cells expressingthe wild-type or mutant PTP1B were assayed for (a) adhesion, (b)spreading, (c) presence of focal adhesions and stress fibers,and (d) tyrosine phosphorylation. Parental cells and cells expressingwild-type PTP1B show similar morphology, are able to attach andspread on fibronectin, and form focal adhesions and stress fibers.In contrast, cells expressing the inactive PTP1B have a spindle-shapedmorphology, reduced adhesion and spreading on fibronectin, andalmost a complete absence of focal adhesions and stress fibers.Attachment to fibronectin induces tyrosine phosphorylation offocal adhesion kinase (FAK) and paxillin in parental cells andcells transfected with the wild-type PTP1B, while in cells transfectedwith the mutant PTP1B, such induction is not observed. Additionally,in cells expressing the mutant PTP1B, tyrosine phosphorylationof Src is enhanced and activity is reduced. Lysophosphatidic acidtemporarily reverses the effects of the mutant PTP1B, suggestingthe existence of a signaling pathway triggering focal adhesionassembly that bypasses the need for active PTP1B. PTP1B coimmunoprecipitateswith $beta$ 1-integrin from nonionic detergent extracts and colocalizeswith vinculin and the ends of actin stress fibers in focal adhesions.Our data suggest that PTP1B is a critical regulatory componentof integrin signaling pathways, which is essential for adhesion,spreading, and formation of focal adhesions.

Key words: protein tyrosine phosphatase; integrins; cell-substrate adhesion; cell signaling; tyrosine phosphorylation

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Cortesio, C. L., Chan, K. T., Perrin, B. J., Burton, N. O., Zhang, S., Zhang, Z.-Y., Huttenlocher, A. (2008). Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion. J. Cell Biol. 180: 957-971 [Abstract] [Full Text]
Jaggi, F., Cabrita, M. A., Perl, A.-K. T., Christofori, G. (2008). Modulation of Endocrine Pancreas Development but not {beta}-Cell Carcinogenesis by Sprouty4. Mol Cancer Res 6: 468-482 [Abstract] [Full Text]
Zhu, S., Bjorge, J. D., Fujita, D. J. (2007). PTP1B Contributes to the Oncogenic Properties of Colon Cancer Cells through Src Activation. Cancer Res. 67: 10129-10137 [Abstract] [Full Text]
Picha, K. M., Patel, S. S., Mandiyan, S., Koehn, J., Wennogle, L. P. (2007). The Role of the C-terminal Domain of Protein Tyrosine Phosphatase-1B in Phosphatase Activity and Substrate Binding. J. Biol. Chem. 282: 2911-2917 [Abstract] [Full Text]
Kabuyama, Y., Langer, S. J., Polvinen, K., Homma, Y., Resing, K. A., Ahn, N. G. (2006). Functional Proteomics Identifies Protein-tyrosine Phosphatase 1B as a Target of RhoA Signaling. Mol. Cell. Proteomics 5: 1359-1367 [Abstract] [Full Text]
Burridge, K., Sastry, S. K., Sallee, J. L. (2006). Regulation of Cell Adhesion by Protein-tyrosine Phosphatases: I. CELL-MATRIX ADHESION. J. Biol. Chem. 281: 15593-15596 [Abstract] [Full Text]
Hernandez, M. V., Sala, M. G. D., Balsamo, J., Lilien, J., Arregui, C. O. (2006). ER-bound PTP1B is targeted to newly forming cell-matrix adhesions. J. Cell Sci. 119: 1233-1243 [Abstract] [Full Text]
Burdon, K. P., Bento, J. L., Langefeld, C. D., Campbell, J. K., Carr, J. J., Wagenknecht, L. M., Herrington, D. M., Freedman, B. I., Rich, S. S., Bowden, D. W. (2006). Association of Protein Tyrosine Phosphatase-N1 Polymorphisms With Coronary Calcified Plaque in the Diabetes Heart Study. Diabetes 55: 651-658 [Abstract] [Full Text]
Chang, Y., Ceacareanu, B., Zhuang, D., Zhang, C., Pu, Q., Ceacareanu, A. C., Hassid, A. (2006). Counter-Regulatory Function of Protein Tyrosine Phosphatase 1B in Platelet-Derived Growth Factor- or Fibroblast Growth Factor-Induced Motility and Proliferation of Cultured Smooth Muscle Cells and in Neointima Formation. Arterioscler. Thromb. Vasc. Bio. 26: 501-507 [Abstract] [Full Text]
Zhang, Z., Lin, S.-Y., Neel, B. G., Haimovich, B. (2006). Phosphorylated {alpha}-Actinin and Protein-tyrosine Phosphatase 1B Coregulate the Disassembly of the Focal Adhesion Kinase{middle dot}Src Complex and Promote Cell Migration. J. Biol. Chem. 281: 1746-1754 [Abstract] [Full Text]
Arias-Salgado, E. G., Haj, F., Dubois, C., Moran, B., Kasirer-Friede, A., Furie, B. C., Furie, B., Neel, B. G., Shattil, S. J. (2005). PTP-1B is an essential positive regulator of platelet integrin signaling. J. Cell Biol. 170: 837-845 [Abstract] [Full Text]
Liang, F., Lee, S.-Y., Liang, J., Lawrence, D. S., Zhang, Z.-Y. (2005). The Role of Protein-tyrosine Phosphatase 1B in Integrin Signaling. J. Biol. Chem. 280: 24857-24863 [Abstract] [Full Text]
Stoker, A. W (2005). Protein tyrosine phosphatases and signalling. J Endocrinol 185: 19-33 [Abstract] [Full Text]
Chang, Y., Zhuang, D., Zhang, C., Hassid, A. (2004). Increase of PTP levels in vascular injury and in cultured aortic smooth muscle cells treated with specific growth factors. Am. J. Physiol. Heart Circ. Physiol. 287: H2201-H2208 [Abstract] [Full Text]
Romanova, L. Y., Hashimoto, S., Chay, K.-O., Blagosklonny, M. V., Sabe, H., Mushinski, J. F. (2004). Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J. Cell Sci. 117: 3759-3768 [Abstract] [Full Text]
Dube, N., Cheng, A., Tremblay, M. L. (2004). The role of protein tyrosine phosphatase 1B in Ras signaling. Proc. Natl. Acad. Sci. USA 101: 1834-1839 [Abstract] [Full Text]
Kuo, J.-C., Lin, J.-R., Staddon, J. M., Hosoya, H., Chen, R.-H. (2003). Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase. J. Cell Sci. 116: 4777-4790 [Abstract] [Full Text]
Dadke, S., Chernoff, J. (2003). Protein-tyrosine Phosphatase 1B Mediates the Effects of Insulin on the Actin Cytoskeleton in Immortalized Fibroblasts. J. Biol. Chem. 278: 40607-40611 [Abstract] [Full Text]
Ragab, A., Bodin, S., Viala, C., Chap, H., Payrastre, B., Ragab-Thomas, J. (2003). The Tyrosine Phosphatase 1B Regulates Linker for Activation of T-cell Phosphorylation and Platelet Aggregation upon Fc{gamma}RIIa Cross-linking. J. Biol. Chem. 278: 40923-40932 [Abstract] [Full Text]
Takino, T., Tamura, M., Miyamori, H., Araki, M., Matsumoto, K., Sato, H., Yamada, K. M. (2003). Tyrosine phosphorylation of the CrkII adaptor protein modulates cell migration. J. Cell Sci. 116: 3145-3155 [Abstract] [Full Text]
Haj, F. G., Markova, B., Klaman, L. D., Bohmer, F. D., Neel, B. G. (2003). Regulation of Receptor Tyrosine Kinase Signaling by Protein Tyrosine Phosphatase-1B. J. Biol. Chem. 278: 739-744 [Abstract] [Full Text]
Zeng, L., Si, X., Yu, W.-P., Le, H. T., Ng, K. P., Teng, R. M.H., Ryan, K., Wang, D. Z.-M., Ponniah, S., Pallen, C. J. (2003). PTP{alpha} regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration. J. Cell Biol. 160: 137-146 [Abstract] [Full Text]
Sastry, S. K., Lyons, P. D., Schaller, M. D., Burridge, K. (2002). PTP-PEST controls motility through regulation of Rac1. J. Cell Sci. 115: 4305-4316 [Abstract] [Full Text]
Abbi, S., Ueda, H., Zheng, C., Cooper, L. A., Zhao, J., Christopher, R., Guan, J.-L. (2002). Regulation of Focal Adhesion Kinase by a Novel Protein Inhibitor FIP200. Mol. Biol. Cell 13: 3178-3191 [Abstract] [Full Text]
Teixeira, J. E., Mann, B. J. (2002). Entamoeba histolytica-Induced Dephosphorylation in Host Cells. Infect. Immun. 70: 1816-1823 [Abstract] [Full Text]
Pixley, F. J., Lee, P. S. W., Condeelis, J. S., Stanley, E. R. (2001). Protein Tyrosine Phosphatase {phi} Regulates Paxillin Tyrosine Phosphorylation and Mediates Colony-Stimulating Factor 1-Induced Morphological Changes in Macrophages. Mol. Cell. Biol. 21: 1795-1809 [Abstract] [Full Text]
Klaman, L. D., Boss, O., Peroni, O. D., Kim, J. K., Martino, J. L., Zabolotny, J. M., Moghal, N., Lubkin, M., Kim, Y.-B., Sharpe, A. H., Stricker-Krongrad, A., Shulman, G. I., Neel, B. G., Kahn, B. B. (2000). Increased Energy Expenditure, Decreased Adiposity, and Tissue-Specific Insulin Sensitivity in Protein-Tyrosine Phosphatase 1B-Deficient Mice. Mol. Cell. Biol. 20: 5479-5489 [Abstract] [Full Text]
Li, H., Leung, T.-C., Hoffman, S., Balsamo, J., Lilien, J. (2000). Coordinate Regulation of Cadherin and Integrin Function by the Chondroitin Sulfate Proteoglycan Neurocan. J. Cell Biol. 149: 1275-1288 [Abstract] [Full Text]
Buensuceso, C. S., O'Toole, T. E. (2000). The Association of CRKII with C3G Can be Regulated by Integrins and Defines a Novel Means to Regulate the Mitogen-activated Protein Kinases. J. Biol. Chem. 275: 13118-13125 [Abstract] [Full Text]
Chiarugi, P., Cirri, P., Taddei, L., Giannoni, E., Camici, G., Manao, G., Raugei, G., Ramponi, G. (2000). The Low Mr Protein-tyrosine Phosphatase Is Involved in Rho-mediated Cytoskeleton Rearrangement after Integrin and Platelet-derived Growth Factor Stimulation. J. Biol. Chem. 275: 4640-4646 [Abstract] [Full Text]
Shen, Y., Lyons, P., Cooley, M., Davidson, D., Veillette, A., Salgia, R., Griffin, J. D., Schaller, M. D. (2000). The Noncatalytic Domain of Protein-tyrosine Phosphatase-PEST Targets Paxillin for Dephosphorylation in Vivo. J. Biol. Chem. 275: 1405-1413 [Abstract] [Full Text]
Ruest, P. J., Roy, S., Shi, E., Mernaugh, R. L., Hanks, S. K. (2000). Phosphospecific Antibodies Reveal Focal Adhesion Kinase Activation Loop Phosphorylation in Nascent and Mature Focal Adhesions and Requirement for the Autophosphorylation Site. Cell Growth Differ. 11: 41-48 [Abstract] [Full Text]
Tamura, M., Gu, J., Tran, H., Yamada, K. M. (1999). PTEN Gene and Integrin Signaling in Cancer. JNCI J Natl Cancer Inst 91: 1820-1828 [Abstract] [Full Text]
Giancotti, F. G., Ruoslahti, E. (1999). Integrin Signaling. Science 285: 1028-1033 [Abstract] [Full Text]
Lee, Y.-J., Streuli, C. H. (1999). Extracellular Matrix Selectively Modulates the Response of Mammary Epithelial Cells to Different Soluble Signaling Ligands. J. Biol. Chem. 274: 22401-22408 [Abstract] [Full Text]
Tamura, M., Gu, J., Danen, E. H. J., Takino, T., Miyamoto, S., Yamada, K. M. (1999). PTEN Interactions with Focal Adhesion Kinase and Suppression of the Extracellular Matrix-dependent Phosphatidylinositol 3-Kinase/Akt Cell Survival Pathway. J. Biol. Chem. 274: 20693-20703 [Abstract] [Full Text]
Ogata, M., Takada, T., Mori, Y., Uchida, Y., Miki, T., Okuyama, A., Kosugi, A., Sawada, M., Oh-hora, M., Hamaoka, T. (1999). Regulation of Phosphorylation Level and Distribution of PTP36, a Putative Protein Tyrosine Phosphatase, by Cell-Substrate Adhesion. J. Biol. Chem. 274: 20717-20724 [Abstract] [Full Text]
Schoenwaelder, S. M., Burridge, K. (1999). Evidence for a Calpeptin-sensitive Protein-tyrosine Phosphatase Upstream of the Small GTPase Rho. A NOVEL ROLE FOR THE CALPAIN INHIBITOR CALPEPTIN IN THE INHIBITION OF PROTEIN-TYROSINE PHOSPHATASES. J. Biol. Chem. 274: 14359-14367 [Abstract] [Full Text]
Ogata, M., Takada, T., Mori, Y., Oh-hora, M., Uchida, Y., Kosugi, A., Miyake, K., Hamaoka, T. (1999). Effects of Overexpression of PTP36, a Putative Protein Tyrosine Phosphatase, on Cell Adhesion, Cell Growth, and Cytoskeletons in HeLa Cells. J. Biol. Chem. 274: 12905-12909 [Abstract] [Full Text]
Angers-Loustau, A., Cote, J.-F., Charest, A., Dowbenko, D., Spencer, S., Lasky, L. A., Tremblay, M. L. (1999). Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts. J. Cell Biol. 144: 1019-1031 [Abstract] [Full Text]
Suen, P., Ilic, D, Caveggion, E, Berton, G, Damsky, C., Lowell, C. (1999). Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages. J. Cell Sci. 112: 4067-4078 [Abstract]
Buist, A., Blanchetot, C., Tertoolen, L. G. J., den Hertog, J. (2000). Identification of p130cas as an in Vivo Substrate of Receptor Protein-tyrosine Phosphatase alpha. J. Biol. Chem. 275: 20754-20761 [Abstract] [Full Text]
Bjorge, J. D., Pang, A., Fujita, D. J. (2000). Identification of Protein-tyrosine Phosphatase 1B as the Major Tyrosine Phosphatase Activity Capable of Dephosphorylating and Activating c-Src in Several Human Breast Cancer Cell Lines. J. Biol. Chem. 275: 41439-41446 [Abstract] [Full Text]
Rhee, J., Lilien, J., Balsamo, J. (2001). Essential Tyrosine Residues for Interaction of the Non-receptor Protein-tyrosine Phosphatase PTP1B with N-cadherin. J. Biol. Chem. 276: 6640-6644 [Abstract] [Full Text]
Cheng, A., Bal, G. S., Kennedy, B. P., Tremblay, M. L. (2001). Attenuation of Adhesion-dependent Signaling and Cell Spreading in Transformed Fibroblasts Lacking Protein Tyrosine Phosphatase-1B. J. Biol. Chem. 276: 25848-25855 [Abstract] [Full Text]
Lyons, P. D., Dunty, J. M., Schaefer, E. M., Schaller, M. D. (2001). Inhibition of the Catalytic Activity of Cell Adhesion Kinase beta by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation. J. Biol. Chem. 276: 24422-24431 [Abstract] [Full Text]
Xu, F., Zhao, R., Peng, Y., Guerrah, A., Zhao, Z. J. (2001). Association of Tyrosine Phosphatase SHP-2 with F-actin at Low Cell Densities. J. Biol. Chem. 276: 29479-29484 [Abstract] [Full Text]
Sorenson, C. M., Sheibani, N. (2002). Altered regulation of SHP-2 and PTP 1B tyrosine phosphatases in cystic kidneys from bcl-2 -/- mice. Am. J. Physiol. Renal Physiol. 282: F442-F450 [Abstract] [Full Text]