Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function

doi:10.1083/jcb.143.4.1029

This Article

	Full Text
	PDF (Full Text)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hofmann, C.
	Articles by Drubin, D. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hofmann, C.
	Articles by Drubin, D. G.


Social Bookmarking

	What's this?

J. Cell Biol., Volume 143, Number 4, November 16, 1998 1029-1040

Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function

Christian Hofmann, Iain M. Cheeseman, Bruce L. Goode, Kent L. McDonald, Georjana Barnes, and David G. Drubin

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202

In this paper, we describe the identification and characterization of two novel and essential mitotic spindle proteins, Duo1pand Dam1p. Duo1p was isolated because its overexpression causeddefects in mitosis and a mitotic arrest. Duo1p was localized byimmunofluorescence, by immunoelectron microscopy, and by taggingwith green fluorescent protein (GFP), to intranuclear spindlemicrotubules and spindle pole bodies. Temperature-sensitive duo1mutants arrest with short spindles. This arrest is dependent onthe mitotic checkpoint. Dam1p was identified by two-hybrid analysisas a protein that binds to Duo1p. By expressing a GFP-Dam1p fusionprotein in yeast, Dam1p was also shown to be associated with intranuclearspindle microtubules and spindle pole bodies in vivo. As withDuo1p, overproduction of Dam1p caused mitotic defects. Biochemicalexperiments demonstrated that Dam1p binds directly to microtubuleswith micromolar affinity. We suggest that Dam1p might localizeDuo1p to intranuclear microtubules and spindle pole bodies toprovide a previously unrecognized function (or functions) requiredfor mitosis.

Key words: mitosis; microtubule; anaphase; cytoskeleton; spindle

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Wong, J., Nakajima, Y., Westermann, S., Shang, C., Kang, J.-s., Goodner, C., Houshmand, P., Fields, S., Chan, C. S.M., Drubin, D., Barnes, G., Hazbun, T. (2007). A Protein Interaction Map of the Mitotic Spindle. Mol. Biol. Cell 18: 3800-3809 [Abstract] [Full Text]
Miranda, J. L., King, D. S., Harrison, S. C. (2007). Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex. Mol. Biol. Cell 18: 2503-2510 [Abstract] [Full Text]
Montpetit, B., Hazbun, T. R., Fields, S., Hieter, P. (2006). Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation. J. Cell Biol. 174: 653-663 [Abstract] [Full Text]
Asbury, C. L., Gestaut, D. R., Powers, A. F., Franck, A. D., Davis, T. N. (2006). The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Proc. Natl. Acad. Sci. USA 103: 9873-9878 [Abstract] [Full Text]
Montpetit, B., Thorne, K., Barrett, I., Andrews, K., Jadusingh, R., Hieter, P., Measday, V. (2005). Genome-Wide Synthetic Lethal Screens Identify an Interaction Between the Nuclear Envelope Protein, Apq12p, and the Kinetochore in Saccharomyces cerevisiae. Genetics 171: 489-501 [Abstract] [Full Text]
Li, J.-m., Li, Y., Elledge, S. J. (2005). Genetic Analysis of the Kinetochore DASH Complex Reveals an Antagonistic Relationship with the Ras/Protein Kinase A Pathway and a Novel Subunit Required for Ask1 Association. Mol. Cell. Biol. 25: 767-778 [Abstract] [Full Text]
Lee, M. S., Spencer, F. A. (2004). Bipolar orientation of chromosomes in Saccharomyces cerevisiae is monitored by Mad1 and Mad2, but not by Mad3. Proc. Natl. Acad. Sci. USA 101: 10655-10660 [Abstract] [Full Text]
Shang, C., Hazbun, T. R., Cheeseman, I. M., Aranda, J., Fields, S., Drubin, D. G., Barnes, G. (2003). Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1p. Mol. Biol. Cell 14: 3342-3355 [Abstract] [Full Text]
Mythreye, K., Bloom, K. S. (2003). Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae. J. Cell Biol. 160: 833-843 [Abstract] [Full Text]
Buvelot, S., Tatsutani, S. Y., Vermaak, D., Biggins, S. (2003). The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. J. Cell Biol. 160: 329-339 [Abstract] [Full Text]
Pot, I., Measday, V., Snydsman, B., Cagney, G., Fields, S., Davis, T. N., Muller, E. G.D., Hieter, P. (2003). Chl4p and Iml3p Are Two New Members of the Budding Yeast Outer Kinetochore. Mol. Biol. Cell 14: 460-476 [Abstract] [Full Text]
Euskirchen, G. M. (2002). Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae. Eukaryot Cell 1: 229-240 [Abstract] [Full Text]
Castillo, A. R., Meehl, J. B., Morgan, G., Schutz-Geschwender, A., Winey, M. (2002). The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. J. Cell Biol. 156: 453-465 [Abstract] [Full Text]
Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241417098v1 [Abstract] [Full Text]
Enquist-Newman, M., Cheeseman, I. M., Van Goor, D., Drubin, D. G., Meluh, P. B., Barnes, G. (2001). Dad1p, Third Component of the Duo1p/Dam1p Complex Involved in Kinetochore Function and Mitotic Spindle Integrity. Mol. Biol. Cell 12: 2601-2613 [Abstract] [Full Text]
Cheeseman, I. M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D. G., Barnes, G. (2001). Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex. J. Cell Biol. 152: 197-212 [Abstract] [Full Text]
Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S., Sakaki, Y. (2000). From the Cover: Toward a protein-protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins. Proc. Natl. Acad. Sci. USA 97: 1143-1147 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 98: 13675-13680 [Abstract] [Full Text]
Kang, J.-s., Cheeseman, I. M., Kallstrom, G., Velmurugan, S., Barnes, G., Chan, C. S.M. (2001). Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. J. Cell Biol. 155: 763-774 [Abstract] [Full Text]
Cheeseman, I. M., Brew, C., Wolyniak, M., Desai, A., Anderson, S., Muster, N., Yates, J. R., Huffaker, T. C., Drubin, D. G., Barnes, G. (2001). Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J. Cell Biol. 155: 1137-1146 [Abstract] [Full Text]
Castillo, A. R., Meehl, J. B., Morgan, G., Schutz-Geschwender, A., Winey, M. (2002). The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. J. Cell Biol. 156: 453-465 [Abstract] [Full Text]