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J. Cell Biol.,
Volume 143, Number 5, November 30, 1998 1239-1247
Department of Pharmacology, University of Medicine and Dentistry of New Jersey The heavy chain of cytoplasmic dynein is required for nuclear migration in Aspergillus nidulans
and other fungi. Here we report on a new gene required for nuclear migration, nudG, which encodes a
homologue of the "8-kD" cytoplasmic dynein light
chain (CDLC). We demonstrate that the temperature
sensitive nudG8 mutation inhibits nuclear migration
and growth at restrictive temperature. This mutation
also inhibits asexual and sexual sporulation, decreases the intracellular concentration of the nudG CDLC protein and causes the cytoplasmic dynein heavy chain to
be absent from the mycelial tip, where it is normally
located in wild-type mycelia. Coimmunoprecipitation experiments with antibodies against the cytoplasmic
dynein heavy chain (CDHC) and the nudG CDLC
demonstrated that some fraction of the cytoplasmic dynein light chain is in a protein complex with the CDHC.
Sucrose gradient sedimentation analysis, however, showed that not all of the NUDG protein is complexed
with the heavy chain. A double mutant carrying a cytoplasmic dynein heavy chain deletion plus a temperature-sensitive nudG mutation grew no more slowly at
restrictive temperature than a strain with only the
CDHC deletion. This result demonstrates that the
effect of the nudG mutation on nuclear migration and
growth is mediated through an interaction with the
CDHC rather than with some other molecule (e.g., myosin-V) with which the 8-kD CDLC might theoretically interact.
Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
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